US4173515A
Expired / Lapsed
US
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Group D enterococci broth
Assignee: McDonnell Douglas Corp
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Filing: 1977-05-02
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Granted: 1979-11-06
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Expires: 1997-05-02
Abstract
Abstract A broth medium for the detection of Group D enterococci in urine. The medium employs neomycin sulfate to inhibit growth of Staphylococcus aureus, potassium tellurite to inhibit growth of gram-negative organisms, and ferric ammonium citrate and esculin hydrolysate indicators.
Inventors
- 👤 Clifton Aldridge
- 👤 Sandra F. Gibson
- 👤 Richard D. Vannest
- 👤 Gregory D. Rodgers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 20:03
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/10; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/305; G01N2333/31
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
67 words
A broth medium for the detection of Group D enterococci in polymicrobic specimen comprising: (a) a nitrogen source, (b) a carbon source, (c) an antibiotic inhibitor to inhibit growth of Staphylococcus aureus, (d) an inhibitor to inhibit growth of gram-negative organisms, and (e) an indicator system which comprises ferric ammonium citrate and esculin hydrolysate whereby metabolic activity of Group D enterococci forms a precipitate in the medium.
Claim 2
Dependent Claim
67 words
A broth medium for the detection of Group D enterococci in polymicrobic specimen comprising: (a) a nitrogen source, (b) a carbon source, (c) an antibiotic inhibitor to inhibit growth of Staphylococcus aureus, (d) an inhibitor to inhibit growth of gram-negative organisms, and (e) an indicator system which comprises ferric ammonium citrate and esculin hydrolysate whereby metabolic activity of Group D enterococci forms a precipitate in the medium.
Claim 3
Dependent Claim
12 words
The medium of claim 1 wherein the antibiotic inhibitor is neomycin sulfate.
Claim 4
Dependent Claim
16 words
The medium of claim 1 wherein about 9800 to about 10,200 mg/l neomycin sulfate is used.
Claim 5
Dependent Claim
12 words
The medium of claim 1 wherein the gram-negative inhibitor is potassium tellurite.
Claim 6
Dependent Claim
20 words
The medium of claim 1 wherein about 0.98 to about 1.02 ml/l of 1% solution of potassium tellurite is used.
Claim 7
Dependent Claim
14 words
The medium of claim 1 wherein the nitrogen source comprises at least two peptones.
Claim 8
Dependent Claim
12 words
The medium of claim 6 wherein the peptones are Trypticase and Phytone.
Claim 9
Dependent Claim
92 words
A broth medium for the detection of Group D enterococci comprising per liter of medium: (a) about 13.5 to about 16.5 gm Trypticase, (b) about 4.5 to about 5.5 gm Phytone, (c) about 0.9 to about 1.1 gm esculin hydrolysate, (d) about 1.8 to about 2.2 gm sodium thioglycollate, (e) about 9800 to about 10,200 mg neomycin sulfate, (f) about 0.98 to about 1.02 ml potassium tellurite, (1% solution) (g) about 0.48 to about 0.52 gm ferric ammonium citrate, and (h) distilled water, (i) said medium having a pH of about 6.6.
Claim 10
Dependent Claim
92 words
A broth medium for the detection of Group D enterococci comprising per liter of medium: (a) about 13.5 to about 16.5 gm Trypticase, (b) about 4.5 to about 5.5 gm Phytone, (c) about 0.9 to about 1.1 gm esculin hydrolysate, (d) about 1.8 to about 2.2 gm sodium thioglycollate, (e) about 9800 to about 10,200 mg neomycin sulfate, (f) about 0.98 to about 1.02 ml potassium tellurite, (1% solution) (g) about 0.48 to about 0.52 gm ferric ammonium citrate, and (h) distilled water, (i) said medium having a pH of about 6.6.
Claim 11
Dependent Claim
64 words
A broth medium for the detection of Group D enterococci comprising per liter of medium: (a) 15.0 gm Trypticase, (b) 5.0 gm Phytone, (c) 1.0 gm esculin hydrolysate, (d) 2.0 gm sodium thioglycollate, (e) 10,000 mg neomycin sulfate, (f) 1.0 ml potassium tellurite (1% solution), (g) 0.5 gm ferric ammonium citrate, and (h) distilled water, (i) said medium having a pH of about 6.6.
Claim 12
Dependent Claim
64 words
A broth medium for the detection of Group D enterococci comprising per liter of medium: (a) 15.0 gm Trypticase, (b) 5.0 gm Phytone, (c) 1.0 gm esculin hydrolysate, (d) 2.0 gm sodium thioglycollate, (e) 10,000 mg neomycin sulfate, (f) 1.0 ml potassium tellurite (1% solution), (g) 0.5 gm ferric ammonium citrate, and (h) distilled water, (i) said medium having a pH of about 6.6.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-11-05 | 1983-05-07 | 1983-11-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-11-05 | 1987-05-07 | 1987-11-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-11-05 | 1991-05-07 | 1991-11-06 | $7700.00 | Window Closed / Lapsed |