US4171430A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Separation of sweet component from natural sweet extracts
Assignee: Tosoh Corp
•
Filing: 1977-08-26
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Granted: 1979-10-16
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Expires: 1997-08-26
Abstract
Abstract Glycosides such as stevioside, glycyrrhizin and neohesperidin dihydrochalcone are separated from non-glycosides such as phyllodulcin and perillartine by column chromatography using a porous gel.
Inventors
- 👤 Susumu Matsushita
- 👤 Tetsuo Ikushige
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 20:30
CPC Classifications
C; C07; C07H; C07H15/00; C07H15/20; C07H15/24; C07H1/00; C07H1/06; C07H1/08; C07H15/256; C07H17/00; C07H17/04; C07H17/06; C07H17/065; C07H17/07
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
72 words
A method of resolving glycoside and non-glycoside sweet components having a molecular weight ranging from 250 to 1,000 in a natural sweet extract, which comprises: passing a solution of said natural sweet extract over a column of a porous organic gel having a pore diameter sufficient to achieve the desired resolution which is less than 1×10 4 A and eluting the resolved glycoside and non-glycoside components from said gel with an eluant.
Claim 2
Independent Claim
72 words
A method of resolving glycoside and non-glycoside sweet components having a molecular weight ranging from 250 to 1,000 in a natural sweet extract, which comprises: passing a solution of said natural sweet extract over a column of a porous organic gel having a pore diameter sufficient to achieve the desired resolution which is less than 1×10 4 A and eluting the resolved glycoside and non-glycoside components from said gel with an eluant.
Claim 3
Dependent Claim
23 words
The method of claim 1, wherein said glycoside is stevioside, glycyrrhizin acid, or neohesperidin dihydrochalcone and said non-glycoside component is phyllodulcin or perillartine.
Claim 4
Dependent Claim
33 words
The method of claim 1, wherein said porous organic gel is a cross-linked styrene gel having a pore diameter of less than 1×10 4 A and said eluent is a non-polar organic solvent.
Claim 5
Dependent Claim
39 words
The method of claim 1, wherein said porous organic gel is a cross-linked starch gel having a pore diameter of less than 1×10 4 A and said eluent is a mixture of water and a water miscible organic solvent.
Claim 6
Dependent Claim
20 words
The method of claim 1, wherein the pore diameter of said porous organic gel is less than 2.5×10× 2 A.
Claim 7
Dependent Claim
25 words
The method of claim 4, wherein said water miscible organic solvent is a C 1 -C 4 alcohol, acetonitrile, dioxane, tetrahydrofuran, dimethylformamide or mixture thereof.
Claim 8
Dependent Claim
18 words
The method of claim 4, wherein said water-organic solvent mixture contains from 4 to 40 weight percent water.
References Cited (Backward Citations)
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US2681907A
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US2738346A
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US3723410A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-10-15 | 1983-04-16 | 1983-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-10-15 | 1987-04-16 | 1987-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-10-15 | 1991-04-16 | 1991-10-16 | $7700.00 | Window Closed / Lapsed |