US4169889A
Expired / Lapsed
US
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Antibiotic bn-200 substance and process for production thereof
Assignee: Meiji Seika Kaisha Ltd
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Filing: 1978-10-04
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Granted: 1979-10-02
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Expires: 1998-10-04
Abstract
Abstract An antibiotic BN-200 substance produced by cultivating a BN-200 substance strain of the genus Erwinia and a process for producing the BN-200 substance.
Inventors
- 👤 Shoichi Amano
- 👤 Shinji Miyadoh
- 👤 Mitsugu Itoh
- 👤 Norio Ezaki
- 👤 Eiichi Akita
- 👤 Yujiro Yamada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:04
CPC Classifications
C; C12; C12P; C12P1/00; C12P1/04; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/847
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
136 words
An antibiotic BN-200 substance having the following properties: Form and Color: white powder Elemental Analysis: C 67.46%, H 7.24%, N 7.55%, O 17.75% (balance); Molecular Weight: 520; determined from the titration value; Melting Point: Begins to turn brown at 150° C., and begins to decompose at 156° C.; Specific Rotatory Power: [α] D 20 =-70.7 (C=1, methanol); Ultraviolet Absorption Spectrum (in methanol): as shown in FIG. 1; Infrared Absorption Spectrum (KBr tablet); as shown in FIG. 2; Solubility in Solvents: Soluble in methanol, ethanol, pyridine, dimethylformamide, acetone, and dimethyl sulfoxide, and insoluble in benzene, ethyl acetate, chloroform, carbon tetrachloride, petroleum ether, and water; Color Reactions: positive with ferric chloride, potassium permanganate and iodine, and negative with ninhydrin, Sakaguchi, biuret, Fehling, Molisch, and anthrone. Rf Values in Silica Gel Thin-Layer Chromatography: Chloroform-Methanol (9:1)--0.57 Benzene-Ethanol (9:1)--0.47 Benzene-Acetone (1:1)--0.76
Claim 2
Dependent Claim
136 words
An antibiotic BN-200 substance having the following properties: Form and Color: white powder Elemental Analysis: C 67.46%, H 7.24%, N 7.55%, O 17.75% (balance); Molecular Weight: 520; determined from the titration value; Melting Point: Begins to turn brown at 150° C., and begins to decompose at 156° C.; Specific Rotatory Power: [α] D 20 =-70.7 (C=1, methanol); Ultraviolet Absorption Spectrum (in methanol): as shown in FIG. 1; Infrared Absorption Spectrum (KBr tablet); as shown in FIG. 2; Solubility in Solvents: Soluble in methanol, ethanol, pyridine, dimethylformamide, acetone, and dimethyl sulfoxide, and insoluble in benzene, ethyl acetate, chloroform, carbon tetrachloride, petroleum ether, and water; Color Reactions: positive with ferric chloride, potassium permanganate and iodine, and negative with ninhydrin, Sakaguchi, biuret, Fehling, Molisch, and anthrone. Rf Values in Silica Gel Thin-Layer Chromatography: Chloroform-Methanol (9:1)--0.57 Benzene-Ethanol (9:1)--0.47 Benzene-Acetone (1:1)--0.76
Claim 3
Dependent Claim
55 words
A process for producing the antibiotic BN-200 substance of claim 1, which comprises aerobically cultivating Erwinia sp. BN-200 strain (FERM-P 3561/ATCC-31420), as a BN-200 substance-producing strain, in a culture medium containing assimilable carbon and nitrogen sources at a temperature of about 20° to about 35° C., and recovering the BN-200 substance from the culture broth.
References Cited (Backward Citations)
US3769403A
Cited
US3819835A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-10-01 | 1983-04-02 | 1983-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-10-01 | 1987-04-02 | 1987-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-10-01 | 1991-04-02 | 1991-10-02 | $7700.00 | Window Closed / Lapsed |