US4164448A
Expired / Lapsed
US
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Activation of cholesterol oxidase for cholesterol assay
Assignee: Roche Diagnostics GmbH
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Filing: 1977-02-14
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Granted: 1979-08-14
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Expires: 1997-02-14
Abstract
Abstract Cholesterol oxidase free of unstabilizing residual traces of detergent is activated by adding to the cholesterol oxidase, before use thereof, at least one surface-active compound with lipophilic and hydrophilic properties. Diagnostic agents for determining cholesterol comprising cholesterol oxidase, a hydrogen peroxide detection system, a buffer, and at least one surface-active compound having lipophilic and hydrophilic properties are provided.
Inventors
- 👤 Peter Roeschlau
- 👤 Gunter Lang
- 👤 Klaus Beaucamp
- 👤 Erich Bernt
- 👤 Wolfgang Gruber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 11:04
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12Q; C12Q1/00; C12Q1/60
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
90 words
Process for the activation of analytically pure, detergent-free, storage-stable cholesterol oxidase, recovered from a micro-organism by extraction with a surfactant, for the analytic determination of cholesterol which process comprises removing all traces of said surfactant from said cholesterol oxidase to produce a surfactant-free cholesterol oxidase and then adding to an aqueous solution of the surfactant-free cholesterol oxidase between 0.005% to 0.1% by weight, based on the weight of the aqueous cholesterol oxidase solution, of at least one surface-active compound with lipophilic and hydrophilic properties before use of said cholesterol oxidase.
Claim 2
Dependent Claim
90 words
Process for the activation of analytically pure, detergent-free, storage-stable cholesterol oxidase, recovered from a micro-organism by extraction with a surfactant, for the analytic determination of cholesterol which process comprises removing all traces of said surfactant from said cholesterol oxidase to produce a surfactant-free cholesterol oxidase and then adding to an aqueous solution of the surfactant-free cholesterol oxidase between 0.005% to 0.1% by weight, based on the weight of the aqueous cholesterol oxidase solution, of at least one surface-active compound with lipophilic and hydrophilic properties before use of said cholesterol oxidase.
Claim 3
Dependent Claim
20 words
Process as claimed in claim 1, wherein the surface-active compound used in non-ionic and contains at least one hydroxyl group.
Claim 4
Dependent Claim
20 words
Process as claimed in claim 2, wherein the non-ionic compound is a polyoxyethylene derivative of an aliphatic or aromatic alcohol.
Claim 5
Dependent Claim
20 words
Process as claimed in claim 1, wherein said surface-active compound is a polyoxyethylene derivative of alkyl, aryl and aralkyl alcohols.
Claim 6
Dependent Claim
14 words
Process as claimed in claim 4, wherein said derivative is a polyoxyethylene alkyl ether.
Claim 7
Dependent Claim
16 words
Process as claimed in claim 4, wherein said derivative is a polyoxyethylene alkyl carboxylic acid ester.
Claim 8
Dependent Claim
16 words
Process as claimed in claim 4, wherein said derivative is a polyoxyethylene-sorbitan alkyl carboxylic acid ester.
Claim 9
Dependent Claim
16 words
Process as claimed in claim 4, wherein said derivative is a polyoxyethylene-glycerol alkyl carboxylic acid ester.
Claim 10
Dependent Claim
12 words
Process as claimed in claim 4, wherein said derivative is a polyoxyethylene-alkylamine.
Claim 11
Dependent Claim
14 words
Process as claimed in claim 4, wherein said derivative is a polyoxyethylene-polyoxypropylene block polymer.
Claim 12
Dependent Claim
13 words
Process as claimed in claim 4, wherein said derivative is a polyoxyethylene-alkyl thioether.
Claim 13
Dependent Claim
15 words
Process as claimed in claim 4, wherein said derivative is a polyoxyethylene alkyl aryl ether.
Claim 14
Dependent Claim
12 words
Process as claimed in claim 1, wherein said surface-active compound is hydroxy-polyethoxydodecane.
Claim 15
Dependent Claim
16 words
Process as claimed in claim 1, wherein said surface-active compound is an ethyleneoxy adduct of alkyl-phenols.
Claim 16
Dependent Claim
17 words
Process as claimed in claim 1, wherein said surface-active compound is a polyethoxy-ethylene derivative of sorbitol anhydrides.
Claim 17
Dependent Claim
22 words
Process as claimed in claim 1 wherein said surface-active compound is used in an amount of from 0.005% to 0.6% by weight.
Claim 18
Dependent Claim
62 words
Diagnostic agent in solid form for the detection and determination of cholesterol and cholesterol esters in body fluids which comprises a solid carrier having impregnated or embedded therein cholesterol oxidase, a system for the detection of hydrogen peroxide, buffer and from 2 to 30%, based on the total solid diagnostic agent of at least one surface-active compound with lipophilic and hydrophilic properties.
Claim 19
Dependent Claim
62 words
Diagnostic agent in solid form for the detection and determination of cholesterol and cholesterol esters in body fluids which comprises a solid carrier having impregnated or embedded therein cholesterol oxidase, a system for the detection of hydrogen peroxide, buffer and from 2 to 30%, based on the total solid diagnostic agent of at least one surface-active compound with lipophilic and hydrophilic properties.
Claim 20
Dependent Claim
16 words
Diagnostic agent as claimed in claim 17, wherein the carrier is impregnated with said surface-active compound.
Claim 21
Dependent Claim
26 words
Diagnostic agent as claimed in claim 17, wherein the surface-active agent is present therein in a concentration of 10 to 20%, referred to the solid reagents.
Claim 22
Dependent Claim
13 words
Diagnostic agent as claimed in claim 17, wherein cholesterol esterase is also present.
Claim 23
Dependent Claim
20 words
Diagnostic agent as claimed in claim 17 wherein at least one of a swelling and stabilizing agent is also present.
Claim 24
Dependent Claim
17 words
Diagnostic agent as claimed in claim 17 wherein said carrier is an absorbent carrier impregnated with cholesteroloxidase.
Claim 25
Dependent Claim
19 words
Diagnostic agent as claimed in claim 17 wherein said carrier is a synthetic resin film having embedded therein cholesteroloxidase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-08-13 | 1983-02-12 | 1983-08-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-08-13 | 1987-02-12 | 1987-08-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-08-13 | 1991-02-12 | 1991-08-14 | $7700.00 | Window Closed / Lapsed |