US4155916A
Expired / Lapsed
US
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Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes
Assignee: Individual
•
Filing: 1977-08-29
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Granted: 1979-05-22
•
Expires: 1997-08-29
Abstract
Abstract Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 5-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.
Inventors
- 👤 Robert E. Smith
- 👤 Frank A. Dolbeare
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 00:33
CPC Classifications
C; C07; C07D; C07D311/00; C07D311/02; C07D311/04; C07D311/06; C07D311/20; C12; C12Q; C12Q1/00
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
17 words
A compound of the formula ##STR1## in which R is selected from the group consisting of ##STR2##
Claim 2
Dependent Claim
17 words
A compound of the formula ##STR1## in which R is selected from the group consisting of ##STR2##
Claim 3
Dependent Claim
9 words
The compound of claim 1 wherein R is ##STR3##
Claim 4
Dependent Claim
9 words
The compound of claim 1 wherein R is ##STR4##
Claim 5
Dependent Claim
9 words
The compound of claim 1 wherein R is ##STR5##
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-05-21 | 1982-11-20 | 1983-05-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-05-21 | 1986-11-20 | 1987-05-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-05-21 | 1990-11-20 | 1991-05-22 | $7700.00 | Window Closed / Lapsed |