US4148870A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Osmium compounds
Assignee: Johnson Matthey PLC
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Filing: 1978-05-15
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Granted: 1979-04-10
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Expires: 1998-05-15
Abstract
Abstract This specification describes a complex of osmium tetroxide and a compound having a heterocyclic ring containing one or two tertiary nitrogen atoms. The compound may be an aromatic tertiary amine and the tertiary nitrogen atom may be the sole heterocyclic atom in the heterocyclic ring. The complexes described are suitable for fixing and staining cells and may be used in the preparation of biological specimens for examination by electron microscopy.
Inventors
- 👤 Paul C. Hydes
- 👤 Michael J. Cleare
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 11:18
CPC Classifications
C; C07; C07D; C07D487/00; C07D487/02; C07D487/08; C07D217/00; C07D217/02; C07D237/00; C07D237/02; C07D237/06; C07D237/08; C07D237/26; C07D237/30; C07D453/00; C07D453/02; C07F; C07F15/00; C07F15/0006; C07F15/002; C07F15/0026
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
69 words
In a method of fixing or staining cells in the preparation of specimens for examination by electron microscopy, in which the cells are treated with osmium tetroxide, the improvement comprising employing for said osmium tetroxide a complex of osmium tetroxide and a compound having a heterocylic ring containing one or two tertiary nitrogen atoms said compound being selected from the group consisting of pyridazine, phthalazine, isoquinoline, triethylenediamine, and quinuclidine.
Claim 2
Dependent Claim
69 words
In a method of fixing or staining cells in the preparation of specimens for examination by electron microscopy, in which the cells are treated with osmium tetroxide, the improvement comprising employing for said osmium tetroxide a complex of osmium tetroxide and a compound having a heterocylic ring containing one or two tertiary nitrogen atoms said compound being selected from the group consisting of pyridazine, phthalazine, isoquinoline, triethylenediamine, and quinuclidine.
Claim 3
Dependent Claim
17 words
A method according to claim 1, in which the complex is in solution in aqueous dimethyl formamide.
Claim 4
Dependent Claim
60 words
In a method of forming osmium blacks in electron microscopic cytochemistry, which comprises treating cells with osmium tetroxide, the improvement comprising employing for said osmium tetroxide a complex of osmium tetroxide and a compound having a heterocyclic ring containing one or two tertiary nitrogen atoms said compound being selected from the group consisting of pyridazine, phthalazine, isoquinoline, triethylenediamine, and quinuclidine.
Claim 5
Dependent Claim
60 words
In a method of forming osmium blacks in electron microscopic cytochemistry, which comprises treating cells with osmium tetroxide, the improvement comprising employing for said osmium tetroxide a complex of osmium tetroxide and a compound having a heterocyclic ring containing one or two tertiary nitrogen atoms said compound being selected from the group consisting of pyridazine, phthalazine, isoquinoline, triethylenediamine, and quinuclidine.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-04-09 | 1982-10-09 | 1983-04-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-04-09 | 1986-10-09 | 1987-04-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-04-09 | 1990-10-09 | 1991-04-10 | $7700.00 | Window Closed / Lapsed |