US4148609A
Expired / Lapsed
US
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Method for measuring antistreptolysin concentration in blood
Assignee: Istituto Sieroterapico e Vaccinogeno Toscano Sclavo SpA
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Filing: 1976-12-02
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Granted: 1979-04-10
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Expires: 1996-12-02
Abstract
Abstract A method for measuring the antistreptolysin concentration in a human blood sample by adding a solution of oxidized 0-streptolysin to the blood sample (diluted), incubating, reacting with a reducing solution, incubating and relating the resulting hemolysis to the antistreptolysin concentration.
Inventors
- 👤 Antonio Ricci
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56911; G01N33/56944; G01N33/543; G01N33/554; G01N33/555; Y; Y10; Y10S; Y10S436/00; Y10S436/815
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
169 words
A method for measuring the antistreptolysin concentration in a sample of human blood, comprising the steps of: (a) preparing a plurality of solutions of different concentrations of oxidized O-streptolysin; (b) preparing a solution of a reducing substance selected from the group consisting of thiols, sodium metabisulfite and sodium sulfite; (c) diluting a blood sample with a nonhemolytic solution; (d) introducing an equal volume of diluted blood in each of the solutions prepared in step (a); (e) incubating the solutions prepared in step (d) at a temperature in the range between room temperature (18° C.-21° C.) and 50° C. for about 15 minutes; (f) adding to the incubating solutions prepared in step (e) a measured volume of the solution prepared in step (b); (g) incubating the solution prepared in step (f) at a temperature in the range between room temperature and 50° C. for sufficient time to cause the sedimentation of the red blood cells to occur, and (h) noting the sample containers in which no hemolysis has taken place.
Claim 2
Independent Claim
169 words
A method for measuring the antistreptolysin concentration in a sample of human blood, comprising the steps of: (a) preparing a plurality of solutions of different concentrations of oxidized O-streptolysin; (b) preparing a solution of a reducing substance selected from the group consisting of thiols, sodium metabisulfite and sodium sulfite; (c) diluting a blood sample with a nonhemolytic solution; (d) introducing an equal volume of diluted blood in each of the solutions prepared in step (a); (e) incubating the solutions prepared in step (d) at a temperature in the range between room temperature (18° C.-21° C.) and 50° C. for about 15 minutes; (f) adding to the incubating solutions prepared in step (e) a measured volume of the solution prepared in step (b); (g) incubating the solution prepared in step (f) at a temperature in the range between room temperature and 50° C. for sufficient time to cause the sedimentation of the red blood cells to occur, and (h) noting the sample containers in which no hemolysis has taken place.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-04-09 | 1982-10-09 | 1983-04-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-04-09 | 1986-10-09 | 1987-04-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-04-09 | 1990-10-09 | 1991-04-10 | $7700.00 | Window Closed / Lapsed |