US4141809A
Expired / Lapsed
US
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Separation process
Assignee: Ricardo AEA Ltd
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Filing: 1978-07-31
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Granted: 1979-02-27
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Expires: 1998-07-31
Abstract
Abstract Continuous flow electrophoresis in an apparatus such as described in U.S. Pat. No. 3,616,453 is stabilized by using a migrant solution containing a sufficient quantity of a liquid of density less than water such as ethanol. Continuous flow electrophoresis may be used, for example, in the fractionation and/or purification of a wide range of biologically active materials.
Inventors
- 👤 Gordon F. Aitchison
- 👤 Patrick Mattock
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 16:51
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44769
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
150 words
A process for the purification and/or separation by continuous flow electrophoresis of an aqueous solution containing components of different electrophoretic mobilities, which comprises (i) injecting the solution, as a migrant solution, into a second aqueous solution, laminarly flowing in an annular separation chamber as a carrier solution for the migrant solution and stabilised by means of an angular velocity gradient, wherein the migrant solution contains sufficient water-miscible organic liquid of density less than 1 g ml -1 at 20° C to reduce its density, relative to the density of the carrier solution, to a level such that injection of the migrant solution into the carrier solution does not give rise to hydrodynamic instability; (ii) applying a constant electric field across the resulting mixture to produce a differential movement of the components of the migrant solution perpendicular to the direction of flow of the layer; and (iii) collecting desired separated components.
Claim 2
Dependent Claim
150 words
A process for the purification and/or separation by continuous flow electrophoresis of an aqueous solution containing components of different electrophoretic mobilities, which comprises (i) injecting the solution, as a migrant solution, into a second aqueous solution, laminarly flowing in an annular separation chamber as a carrier solution for the migrant solution and stabilised by means of an angular velocity gradient, wherein the migrant solution contains sufficient water-miscible organic liquid of density less than 1 g ml -1 at 20° C to reduce its density, relative to the density of the carrier solution, to a level such that injection of the migrant solution into the carrier solution does not give rise to hydrodynamic instability; (ii) applying a constant electric field across the resulting mixture to produce a differential movement of the components of the migrant solution perpendicular to the direction of flow of the layer; and (iii) collecting desired separated components.
Claim 3
Dependent Claim
15 words
A process according to claim 1 wherein the water-miscible organic liquid is methanol or ethanol.
Claim 4
Dependent Claim
33 words
A process according to claim 2 wherein the injection of the migrant solution is effected at the inner side of the flow of the carrier solution, and its direction of migration is centrifugal.
Claim 5
Dependent Claim
11 words
A process according to claim 3 wherein the flow is helical.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-02-26 | 1982-08-28 | 1983-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-02-26 | 1986-08-28 | 1987-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-02-26 | 1990-08-28 | 1991-02-27 | $7700.00 | Window Closed / Lapsed |