US4141688A
Expired / Lapsed
US
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Composition, device and method for determining reducing agents
Assignee: Bayer Corp
•
Filing: 1977-08-11
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Granted: 1979-02-27
•
Expires: 1997-08-11
Abstract
Abstract A composition, device and method for determining the presence of a reducing agent, such as ascorbic acid, in a test sample are disclosed. The composition comprises certain thiazine and oxazine compounds which, in their oxidized state, are colored, but which become colorless when reduced by a reducing agent. The device comprises a carrier matrix incorporated with the composition, and the method comprises contacting a test sample with the composition device and observing any detectable response such as a color change.
Inventors
- 👤 David A. N. Morris
- 👤 Patricia A. Rupchock
- 👤 Mark T. Skarstedt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 22:26
CPC Classifications
C; C07; C07D; C07D241/00; C07D241/36; C07D241/38; C07D241/46; C07D265/00; C07D265/28; C07D265/34; C07D265/38; C07D279/00; C07D279/10; C07D279/14; C07D279/18; C09; C09B; C09B19/00; C09B21/00; C09B67/00; C09B67/0071; C09B67/0075; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/82; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/20; Y10T436/200833; Y10T436/201666
Claims Analysis
Total Claims: 64
Claim 1
Independent Claim
38 words
A composition for determining the presence of ascorbic acid in a test sample, said composition comprising (a) an indicator compound comprising Lauth's Violet, azur A, methylene green, toluidine blue, gallocyanine, or prune pure, and (b) a suitable buffer.
Claim 2
Dependent Claim
38 words
A composition for determining the presence of ascorbic acid in a test sample, said composition comprising (a) an indicator compound comprising Lauth's Violet, azur A, methylene green, toluidine blue, gallocyanine, or prune pure, and (b) a suitable buffer.
Claim 3
Dependent Claim
12 words
The composition of claim 1 in which the indicator is methylene green.
Claim 4
Dependent Claim
11 words
The composition of claim 1 in which the indicator is gallocyanine.
Claim 5
Dependent Claim
12 words
The composition of claim 1 in which the indicator is prune pure.
Claim 6
Independent Claim
158 words
A device for determining the presence of ascorbic acid in a test sample, the device comprising a carrier matrix incorporated with (a) an indicator compound responsive to ascorbic acid, and having the structure ##STR25## in which X is O or S, wherein when X is S R, R' and R", same or different, are H or lower alkyl, Q is NH 2 or N(lower alkyl) 2 , and G is H, NO 2 or OH; with the proviso that when X is S, Q is not N(CH 3 ) 2 , R is not methyl and G, R' and R" are not H, simultaneously; and when X is O R and R', same or different, are H or lower alkyl, R" is H, COOH, COOR, CONR 2 or R" and R' together form a hydrocarbon ring having 4 to 7 carbon atoms, and G and Q, same or different, are H or OH, and (b) a suitable buffer.
Claim 7
Independent Claim
158 words
A device for determining the presence of ascorbic acid in a test sample, the device comprising a carrier matrix incorporated with (a) an indicator compound responsive to ascorbic acid, and having the structure ##STR25## in which X is O or S, wherein when X is S R, R' and R", same or different, are H or lower alkyl, Q is NH 2 or N(lower alkyl) 2 , and G is H, NO 2 or OH; with the proviso that when X is S, Q is not N(CH 3 ) 2 , R is not methyl and G, R' and R" are not H, simultaneously; and when X is O R and R', same or different, are H or lower alkyl, R" is H, COOH, COOR, CONR 2 or R" and R' together form a hydrocarbon ring having 4 to 7 carbon atoms, and G and Q, same or different, are H or OH, and (b) a suitable buffer.
Claim 8
Dependent Claim
10 words
The device of claim 5 in which X is S.
Claim 9
Dependent Claim
20 words
The device of claim 5 in which the indicator compound is Lauth's violet, azur A, methylene green or toluidine blue.
Claim 10
Dependent Claim
13 words
The device of claim 5 in which the indicator compound is methylene green.
Claim 11
Dependent Claim
10 words
The device of claim 5 in which X is O.
Claim 12
Dependent Claim
21 words
The device of claim 5 in which the indicator compound is gallocyanine, prune pure, gallamine blue, celestine blue or Meldola blue.
Claim 13
Dependent Claim
12 words
The device of claim 5 in which the indicator compound is gallocyanine.
Claim 14
Dependent Claim
13 words
The device of claim 5 in which the indicator compound is prune pure.
Claim 15
Dependent Claim
17 words
The device of claim 5 in which the carrier matrix is further incorporated with a background dye.
Claim 16
Dependent Claim
10 words
The device of claim 13 in which X is S.
Claim 17
Dependent Claim
19 words
The device of claim 13 in which the indicator is Lauth's violet, azur A, methylene green or toluidine blue.
Claim 18
Dependent Claim
12 words
The device of claim 13 in which the indicator is methylene green.
Claim 19
Dependent Claim
19 words
The device of claim 13 in which the indicator is methylene green and the background dye is neutral red.
Claim 20
Dependent Claim
10 words
The device of claim 13 in which X is O.
Claim 21
Dependent Claim
21 words
The device of claim 13 in which the indicator compound is gallocyanine, prune pure, gallamine blue, celestine blue or Meldola blue.
Claim 22
Dependent Claim
12 words
The device of claim 13 in which the indicator compound is gallocyanine.
Claim 23
Dependent Claim
13 words
The device of claim 13 in which the indicator compound is prune pure.
Claim 24
Dependent Claim
19 words
The device of claim 13 in which the indicator compound is gallocyanine and the background dye is neutral red.
Claim 25
Independent Claim
60 words
A device for determining the presence of ascorbic acid in a test sample comprising a bibulous paper carrier matrix affixed to a support member serving as a handle, said matrix being incorporated with methylene green as an indicator compound responsive to ascorbic acid, neutral red as a background dye and a buffer capable of producing a pH of about 5.8.
Claim 26
Independent Claim
60 words
A device for determining the presence of ascorbic acid in a test sample comprising a bibulous paper carrier matrix affixed to a support member serving as a handle, said matrix being incorporated with methylene green as an indicator compound responsive to ascorbic acid, neutral red as a background dye and a buffer capable of producing a pH of about 5.8.
Claim 27
Independent Claim
167 words
A method for determining the presence of ascorbic acid in a test sample comprising (a) contacting the sample with a carrier matrix incorporated with: (i) an indicator compound responsive to ascorbic acid, and having the structure ##STR26## in which X is O or S, wherein when X is S R, R' and R", same or different, are H or lower alkyl, Q is NH 2 or N(lower alkyl) 2 , and G is H, NO 2 or OH; with the proviso that when X is S, Q is not N(CH 3 ) 2 , R is not methyl and G, R' and R" are not H, simultaneously; and when X is O; R and R', same or different, are H or lower alkyl, R" is H, COOH, COOR, CONR 2 or R" and R' together form a hydrocarbon ring having 4 to 7 carbon atoms, and G and Q, same or different, are H or OH; and (ii) a suitable buffer; and (b) observing a detectable response.
Claim 28
Independent Claim
167 words
A method for determining the presence of ascorbic acid in a test sample comprising (a) contacting the sample with a carrier matrix incorporated with: (i) an indicator compound responsive to ascorbic acid, and having the structure ##STR26## in which X is O or S, wherein when X is S R, R' and R", same or different, are H or lower alkyl, Q is NH 2 or N(lower alkyl) 2 , and G is H, NO 2 or OH; with the proviso that when X is S, Q is not N(CH 3 ) 2 , R is not methyl and G, R' and R" are not H, simultaneously; and when X is O; R and R', same or different, are H or lower alkyl, R" is H, COOH, COOR, CONR 2 or R" and R' together form a hydrocarbon ring having 4 to 7 carbon atoms, and G and Q, same or different, are H or OH; and (ii) a suitable buffer; and (b) observing a detectable response.
Claim 29
Dependent Claim
10 words
The method of claim 24 in which X is S.
Claim 30
Dependent Claim
20 words
The method of claim 24 in which the indicator compound is Lauth's violet, azur A, methylene green or toluidine blue.
Claim 31
Dependent Claim
13 words
The method of claim 24 in which the indicator compound is methylene green.
Claim 32
Dependent Claim
10 words
The method of claim 24 in which X is O.
Claim 33
Dependent Claim
21 words
The method of claim 24 in which the indicator compound is gallocyanine, prune pure, gallamine blue, celestine blue or Meldola blue.
Claim 34
Dependent Claim
12 words
The method of claim 24 in which the indicator compound is gallocyanine.
Claim 35
Dependent Claim
13 words
The method of claim 24 in which the indicator compound is prune pure.
Claim 36
Dependent Claim
17 words
The method of claim 24 in which the carrier matrix is further incorporated with a background dye.
Claim 37
Dependent Claim
10 words
The method of claim 32 in which X is S.
Claim 38
Dependent Claim
19 words
The method of claim 32 in which the indicator is Lauth's violet, azur A, methylene green or toluidine blue.
Claim 39
Dependent Claim
12 words
The method of claim 32 in which the indicator is methylene green.
Claim 40
Dependent Claim
19 words
The method of claim 32 in which the indicator is methylene green and the background dye is neutral red.
Claim 41
Dependent Claim
10 words
The method of claim 32 in which X is O.
Claim 42
Dependent Claim
21 words
The method of claim 32 in which the indicator compound is gallocyanine, prune pure, gallamine blue, celestine blue or Meldola blue.
Claim 43
Dependent Claim
12 words
The method of claim 32 in which the indicator compound is gallocyanine.
Claim 44
Dependent Claim
13 words
The method of claim 32 in which the indicator compound is prune pure.
Claim 45
Dependent Claim
19 words
The method of claim 32 in which the indicator compound is gallocyanine and the background dye is neutral red.
Claim 46
Independent Claim
164 words
A method for determining the presence of ascorbic acid in a test sample comprising (a) contacting the sample with a test composition comprising (i) an indicator compound responsive to ascorbic acid, and having the structure ##STR27## in which X is O or S, wherein when X is S R, R' and R", same or different, are H or lower alkyl, Q is NH 2 or N(lower alkyl) 2 , and G is H, NO 2 or OH; with the proviso that when X is S, Q is not N(CH 3 ) 2 , R is not methyl and G, R' and R" are not H, simultaneously; and when X is O R and R', or different, are H or lower alkyl, R" is H, COOH, COOR, CONR 2 or R" and R' together form a hydrocarbon ring having 4to 7 carbon atoms, and G and Q, same or different, are H or OH, and (ii) a suitable buffer; and (b) observing a detectable response.
Claim 47
Independent Claim
164 words
A method for determining the presence of ascorbic acid in a test sample comprising (a) contacting the sample with a test composition comprising (i) an indicator compound responsive to ascorbic acid, and having the structure ##STR27## in which X is O or S, wherein when X is S R, R' and R", same or different, are H or lower alkyl, Q is NH 2 or N(lower alkyl) 2 , and G is H, NO 2 or OH; with the proviso that when X is S, Q is not N(CH 3 ) 2 , R is not methyl and G, R' and R" are not H, simultaneously; and when X is O R and R', or different, are H or lower alkyl, R" is H, COOH, COOR, CONR 2 or R" and R' together form a hydrocarbon ring having 4to 7 carbon atoms, and G and Q, same or different, are H or OH, and (ii) a suitable buffer; and (b) observing a detectable response.
Claim 48
Dependent Claim
10 words
The method of claim 42 in which X is S.
Claim 49
Dependent Claim
20 words
The method of claim 42 in which the indicator compound is Lauth's violet, azur A, methylene green or toluidine blue.
Claim 50
Dependent Claim
13 words
The method of claim 42 in which the indicator compound is methylene green.
Claim 51
Dependent Claim
10 words
The method of claim 42 in which X is O.
Claim 52
Dependent Claim
21 words
The method of claim 42 in which the indicator compound is gallocyanine, prune pure, gallamine blue, celestine blue or Meldola blue.
Claim 53
Dependent Claim
12 words
The method of claim 42 in which the indicator compound is gallocyanine.
Claim 54
Dependent Claim
13 words
The method of claim 42 in which the indicator compound is prune pure.
Claim 55
Dependent Claim
17 words
The method of claim 42 in which the carrier matrix is further incorporated with a background dye.
Claim 56
Dependent Claim
10 words
The method of claim 50 in which X is S.
Claim 57
Dependent Claim
19 words
The method of claim 50 in which the indicator is Lauth's violet, azur A, methylene green or toluidine blue.
Claim 58
Dependent Claim
12 words
The method of claim 50 in which the indicator is methylene green.
Claim 59
Dependent Claim
19 words
The method of claim 50 in which the indicator is methylene green and the background dye is neutral red.
Claim 60
Dependent Claim
10 words
The method of claim 50 in which X is O.
Claim 61
Dependent Claim
21 words
The method of claim 50 in which the indicator compound is gallocyanine, prune pure, gallamine blue, celestine blue or Meldola blue.
Claim 62
Dependent Claim
12 words
The method of claim 50 in which the indicator compound is gallocyanine.
Claim 63
Dependent Claim
13 words
The method of claim 50 in which the indicator compound is prune pure.
Claim 64
Dependent Claim
19 words
The method of claim 50 in which the indicator compound is gallocyanine and the background dye is neutral red.
References Cited (Backward Citations)
US3791988A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-02-26 | 1982-08-28 | 1983-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-02-26 | 1986-08-28 | 1987-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-02-26 | 1990-08-28 | 1991-02-27 | $7700.00 | Window Closed / Lapsed |