Nucleic acid and protein binding paper

Claim 2

A modified cellulosic paper comprising a sheet or continuous web of cellulosic fibers substituted with meta-aminobenzyloxymethyl groups, said modified paper adapted to be activated by diazotization for covalent binding thereto of nucleic acid residues, proteins or mixtures thereof.

Claim 3

The modified paper of claim 1 substituted with diazotized meta-aminobenzyloxymethyl groups.

Claim 4

The paper of claim 1 covalently bonded with nucleic acid residues.

Claim 5

The paper of claim 3 wherein said nucleic acid residues are denatured DNA residues.

Claim 6

The paper of claim 3 wherein said nucleic acid residues are RNA residues.

Claim 7

The paper of claim 1 covalently bonded with protein chains.

Claim 8

The paper of claim 4 hybridized with isotopelabeled RNA residues.

Claim 9

The paper of claim 5 hybridized with isotopelabeled DNA residues.

Claim 10

A process for preparing a modified cellulosic paper adapted for activation by diazotization and subsequent covalent bonding thereto by nucleic acid residues, proteins or mixtures thereof comprising treating a sheet or continuous web of cellulosic fibers with 1-[(meta-nitrobenzyloxy) methyl] pyridinium chloride and thereafter recovering a meta-nitrobenzyloxymethyl substituted sheet or web, then reducing said nitrobenzyloxymethyl substituents to aminobenzyloxymethyl substituents and thereafter recovering said modified cellulosic paper.

Claim 11

A process for preparing a modified cellulosic paper adapted for activation by diazotization and subsequent covalent bonding thereto by nucleic acid residues, proteins or mixtures thereof comprising treating a sheet or continuous web of cellulosic fibers with 1-[(meta-nitrobenzyloxy) methyl] pyridinium chloride and thereafter recovering a meta-nitrobenzyloxymethyl substituted sheet or web, then reducing said nitrobenzyloxymethyl substituents to aminobenzyloxymethyl substituents and thereafter recovering said modified cellulosic paper.

Claim 12

The process of claim 9 including diazotizing the amino moieties of said aminobenzyloxymethyl substituents to form a diazotized paper and thereafter contacting said diazotized paper with a nucleic acid residue or a protein chaine to immobilize said chain or residue.

Claim 13

The process of claim 9 wherein said nucleic acid residue is a DNA or RNA residue.

Claim 14

The process of claim 11 wherein said DNA or RNA residue is hybridized to a corresponding isotope labeled RNA or DNA probe residue to detect the size and identity of the immobilized residue.

Claim 15

A modified cellulose paper substituted with diazotizable functional groups for covalently binding nucleic acid residues, proteins or mixtures thereof.

Claim 16

A modified cellulose paper substituted with diazotizable functional groups for covalently binding nucleic acid residues, proteins or mixtures thereof.

Claim 17

A process for preparing a modified cellulose paper adapted for activation by diazotization and subsequent covalent bonding thereto by nucleic acid residues, proteins or mixtures thereof comprising treating a cellulosic paper with a reagent to form covalently bonded substituents having a meta-nitro substituted aryl group, reducing the nitro groups to amino groups and recovering the modified paper.

Claim 18

A process for preparing a modified cellulose paper adapted for activation by diazotization and subsequent covalent bonding thereto by nucleic acid residues, proteins or mixtures thereof comprising treating a cellulosic paper with a reagent to form covalently bonded substituents having a meta-nitro substituted aryl group, reducing the nitro groups to amino groups and recovering the modified paper.