US4133639A
Expired / Lapsed
US
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Test article including a covalently attached diagnostic reagent and method
Assignee: Lonza Walkersville Inc
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Filing: 1976-03-04
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Granted: 1979-01-09
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Expires: 1996-03-04
Abstract
Abstract A body suitable for use in the quantitative detection of an unknown quantity of a biologically derived sample suitably by radioimmunoassay, fluorometric detection, or by spectrophotometry. The body includes a handle attached to a nonparticulate, nonswellable, impermeable continuous surface region. A diagnostic reagent (e.g., an antibody) is covalently attached to the surface region. The surface region is stirred by the handle in a solution of sample substance (e.g., antigen) for reaction with the diagnostic reagent. In a competitive binding technique, a labelled substance (e.g., fluorochrome labelled antigen) is simultaneously contacted with the sample. Thereafter, the surface region is washed, and the label is measured as by a fluorometer. The technique is also applicable to the so-called "sandwich technique" in which the labelled substance (e.g., labelled antibody) is reacted with the sample substance after it has been reacted with the diagnostic reagent. The above type of surface facilitates simple and effective separation of bound from free labelled substance and allows thorough washing to cleanse the surface of residual free and non-specifically bound labelled substance.
Inventors
- 👤 Richard A. Harte
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:52
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/815; Y10S436/819
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
66 words
A body suitable for use in the fluorometric detection of a biologically derived sample, said body comprising handle means connected to at least one nonparticulate, nonswellable, impermeable, continuous, flat surface region defining an area of at least one square millimeter and a diagnostic reagent bound to at least one biologically derived sample labelled with a fluorochrome, said diagnostic reagent being covalently attached to said surface region.
Claim 2
Dependent Claim
66 words
A body suitable for use in the fluorometric detection of a biologically derived sample, said body comprising handle means connected to at least one nonparticulate, nonswellable, impermeable, continuous, flat surface region defining an area of at least one square millimeter and a diagnostic reagent bound to at least one biologically derived sample labelled with a fluorochrome, said diagnostic reagent being covalently attached to said surface region.
Claim 3
Dependent Claim
44 words
The body of claim 1 in which said surface region includes a plurality of said labelled sample substances attached to said diagnostic reagent in random dispersion, at least two of the fluorochrome labels for said sample emitting fluorescence responsive to different wavelengths of light.
Claim 4
Dependent Claim
23 words
The body of claim 1 in combination with a fluorometer, the receiving end of said fluorometer being adjacent to said flat surface region.
Claim 5
Dependent Claim
48 words
The body of claim 1 together with a second separate nonparticulate, nonswellable, impermeable, continuous, flat surface region covalently attached to one other diagnostic reagent bound to at least one biologically derived sample labelled with a fluorochrome, said other diagnostic reagent being covalently attached to said second surface region.
Claim 6
Dependent Claim
26 words
The body of claim 4 in which said second region comprises a standard region including a predetermined quantity of said fluorochrome labelled sample type of substance.
Claim 7
Dependent Claim
30 words
The body of claim 5 in which said surface region is polyhedral and has a different one of said labelled sample type of substances on a plurality of its face.
Claim 8
Dependent Claim
134 words
In a method for the fluorometric detection of an unknown quantity of a biologically derived sample, the steps of: (a) covalently attaching a diagnostic reagent to at least one nonparticulate, nonswellable, impermeable continuous, flat surface region of a body, (b) contacting the reagent-bonded flat surface of step (a) with a sample substance reactive with said diagnostic reagent in solution and with a fluorochrome labelled substance in solution for a sufficient time to form a fluorochrome labelled reaction product capable of detection, (c) separating the reacted surface and solution containing unbound fluorochrome labelled substance after completion of step (b), (d) washing the reacted surface of step (c); and (e) transporting the body to the detection station of a fluorometer to quantitatively measure the amount of fluorescence emitted from the washed reacted surface of step (d).
Claim 9
Dependent Claim
134 words
In a method for the fluorometric detection of an unknown quantity of a biologically derived sample, the steps of: (a) covalently attaching a diagnostic reagent to at least one nonparticulate, nonswellable, impermeable continuous, flat surface region of a body, (b) contacting the reagent-bonded flat surface of step (a) with a sample substance reactive with said diagnostic reagent in solution and with a fluorochrome labelled substance in solution for a sufficient time to form a fluorochrome labelled reaction product capable of detection, (c) separating the reacted surface and solution containing unbound fluorochrome labelled substance after completion of step (b), (d) washing the reacted surface of step (c); and (e) transporting the body to the detection station of a fluorometer to quantitatively measure the amount of fluorescence emitted from the washed reacted surface of step (d).
Claim 10
Dependent Claim
47 words
The method of claim 7 in which in step (c) sample substance contact is performed by gripping the body by a handle attached thereto, contacting the diagnostic reagent-bonded surface with a container of solution of sample substance, and moving said surface relative to said body of solution.
Claim 11
Dependent Claim
26 words
The method of claim 7 in which the body is dipped into said container of solution and movement is performed by stirring with the handle portion.
Claim 12
Dependent Claim
25 words
The method of claim 7 in which the body is dipped into said container of solution and said movement is performed by agitating said container.
Claim 13
Dependent Claim
37 words
The method of claim 7 in which in step (c) said sample substance is reacted with said diagnostic reagent and washed prior to reaction with said labelled substance, said latter substance being reactive with said sample substance.
Claim 14
Dependent Claim
32 words
The method of claim 7 in which in step (c) said sample substance is simultaneously reacted with said diagnostic reagent and labelled substance, said latter substance being reactive with said diagnostic reagent.
Claim 15
Dependent Claim
126 words
In a method for the fluorometric detection of an unknown quantity of a biologically derived sample, the steps of: (a) covalently attaching a diagnostic reagent to at least one nonparticulate, nonswellable, impermeable continuous, flat surface region of a body, (b) contacting the reagent-bonded surface of step (a) with a fluorescently labelled sample substance reactive with said diagnostic reagent in solution for a sufficient time to form a fluorescent reaction product capable of fluorometric detection, (c) separating the reacted surface and solution containing unbound labelled sample substance after completion of step (b), (d) washing the reacted surface of step (c); and (e) transporting the body to the detection station of a fluorometer to measure the amount of labelled substance on the washed reacted surface of step (d).
Claim 16
Dependent Claim
126 words
In a method for the fluorometric detection of an unknown quantity of a biologically derived sample, the steps of: (a) covalently attaching a diagnostic reagent to at least one nonparticulate, nonswellable, impermeable continuous, flat surface region of a body, (b) contacting the reagent-bonded surface of step (a) with a fluorescently labelled sample substance reactive with said diagnostic reagent in solution for a sufficient time to form a fluorescent reaction product capable of fluorometric detection, (c) separating the reacted surface and solution containing unbound labelled sample substance after completion of step (b), (d) washing the reacted surface of step (c); and (e) transporting the body to the detection station of a fluorometer to measure the amount of labelled substance on the washed reacted surface of step (d).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-01-08 | 1982-07-10 | 1983-01-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-01-08 | 1986-07-10 | 1987-01-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-01-08 | 1990-07-10 | 1991-01-09 | $7700.00 | Window Closed / Lapsed |