US4132527A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic compositions, diagnosing instruments, and methods of manufacturing same
Assignee: Shionogi and Co Ltd
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Filing: 1976-07-26
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Granted: 1979-01-02
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Expires: 1996-07-26
Abstract
Abstract A diagnostic composition containing as a chromogen a diamino-biphenyl derivative in which at least one of the hydrogen atoms in the amino groups thereof is replaced by a lower alkyl group by reaction with an alkylating agent. The diagnosing instrument is prepared by impregnating a bibulous, porous support in a solution comprising the diagnostic composition and optionally a saccharide oxidase, peroxidase and a guaiac, and, if necessary, a buffer, a surfactant, a background adjusting dye and/or an agent for increasing the viscosity of the composition. This diagnosing instrument can be handled in a simple and convenient way and is used to identify semi-quantitatively a saccharide in body fluids or excrements of humans or animals with reliability.
Inventors
- 👤 Hideyuki Maekawa
- 👤 Kaoru Ishitobi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 08:11
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/72; G01N33/721; G01N33/725; C12Q2326/00; C12Q2326/10; C12Q2326/14; Y; Y10; Y10S; Y10S436/00; Y10S436/904
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
67 words
A diagnosing instrument for detecting or measuring occult bleeding in urine or solid excrement comprising a mixture of diamino-biphenyl derivatives having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms, supported on a bibulous, porous support.
Claim 2
Dependent Claim
67 words
A diagnosing instrument for detecting or measuring occult bleeding in urine or solid excrement comprising a mixture of diamino-biphenyl derivatives having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms, supported on a bibulous, porous support.
Claim 3
Dependent Claim
74 words
A diagnosing instrument for detecting or measuring occult bleeding in urine or solid excrement comprising (1) a mixture of diamino-biphenyl derivatives having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms and (2) a non-alkylated diamino-biphenyl derivative, supported on a bibulous, porous support.
Claim 4
Dependent Claim
74 words
A diagnosing instrument for detecting or measuring occult bleeding in urine or solid excrement comprising (1) a mixture of diamino-biphenyl derivatives having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms and (2) a non-alkylated diamino-biphenyl derivative, supported on a bibulous, porous support.
Claim 5
Dependent Claim
19 words
The diagnosing instrument according to claim 2, wherein the lower alkyl group has from one to two carbon atoms.
Claim 6
Dependent Claim
18 words
The diagnosing instrument according to claim 2, wherein the diamino-biphenyl derivative is N-methyl-, N,N-dimethyl-, N,N'-dimethyl-, N,N,N'-trimethyl- or N,N,N',N'-tetramethyl-ortho-tolidine.
Claim 7
Dependent Claim
18 words
The diagnosing instrument according to claim 2, wherein the diamino-biphenyl derivative is N-ethyl, N,N-diethyl-, N,N'-diethyl-, N,N,N'-triethyl- or N,N,N',N'-tetraethyl-ortho-tolidine.
Claim 8
Dependent Claim
29 words
The diagnosing instrument according to claim 2, wherein the bibulous, porous support is filter paper, hardened filter paper, a sintered porous glass material or a sintered porous plastic material.
Claim 9
Dependent Claim
45 words
The diagnosing instrument according to claim 2, wherein the diamino-biphenyl derivative is tolidine in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms.
Claim 10
Dependent Claim
45 words
The diagnosing instrument according to claim 2, wherein the diamino-biphenyl derivative is ortho-tolidine in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms.
Claim 11
Dependent Claim
12 words
The diagnosing instrument according to claim 2, which additionally contains a guaiac.
Claim 12
Dependent Claim
16 words
The diagnosing instrument according to claim 9, wherein the guaiac is alpha-guaiaconic acid or beta-guaiaconic acid.
Claim 13
Dependent Claim
28 words
The diagnosing instrument according to claim 2, which additionally contains a buffer, a surfactant, a background adjusting dye or an agent for increasing the viscosity of the composition.
Claim 14
Dependent Claim
22 words
The diagnosing instrument according to claim 11, wherein the buffer is an aqueous solution of phthalic acid, a phosphate or citric acid.
Claim 15
Dependent Claim
14 words
The diagnosing instrument according to claim 11, wherein the background adjusting dye is tartrazine.
Claim 16
Dependent Claim
18 words
The diagnosing instrument according to claim 11, wherein the agent for increasing the viscosity is a protective colloid.
Claim 17
Dependent Claim
18 words
The diagnosing instrument according to claim 14, wherein the protective colloid is a polyalkylene glycol or polyvinyl alcohol.
Claim 18
Dependent Claim
102 words
A diagnostic method for detecting or measuring occult bleeding in urine or solid excrement which comprises applying a specimen of urine or solid excrement onto an instrument comprising (1) a mixture of diamino-biphenyl derivatives having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms and (2) a non-alkylated diamino-biphenyl derivative as a chromogen supported on a bibulous, porous support, applying hydrogen peroxide thereto and observing the color change of the instrument.
Claim 19
Dependent Claim
102 words
A diagnostic method for detecting or measuring occult bleeding in urine or solid excrement which comprises applying a specimen of urine or solid excrement onto an instrument comprising (1) a mixture of diamino-biphenyl derivatives having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms and (2) a non-alkylated diamino-biphenyl derivative as a chromogen supported on a bibulous, porous support, applying hydrogen peroxide thereto and observing the color change of the instrument.
Claim 20
Dependent Claim
19 words
The diagnostic method according to claim 16, wherein the lower alkyl group has from one to two carbon atoms.
Claim 21
Dependent Claim
17 words
The diagnostic method according to claim 16, wherein the diamino-biphenyl derivative is N-methyl-, N,N'-dimethyl-, N,N,N'-trimethyl- or N,N,N',N'-tetramethyl-ortho-tolidine.
Claim 22
Dependent Claim
19 words
The diagnostic method according to claim 16, wherein the diamino-biphenyl derivative is N-ethyl-, N,N-diethyl-, N,N'- diethyl-, N,N,N'-triethyl- or N,N,N',N'-tetraethyl-ortho-tolidine.
Claim 23
Dependent Claim
30 words
The diagnostic method according to claim 16, wherein said instrument additionally contains a buffer, a surfactant, a background adjusting dye or an agent for increasing the viscosity of the composition.
Claim 24
Dependent Claim
22 words
The diagnostic method according to claim 10, wherein the buffer is an aqueous solution of phthalic acid, a phosphate or citric acid.
Claim 25
Dependent Claim
14 words
The diagnostic method according to claim 16, wherein the background adjusting dye is tartrazine.
Claim 26
Dependent Claim
29 words
The diagnostic method according to claim 16, wherein the bibulous, porous support is filter paper, hardened filter paper, a sintered porous glass material or a sintered porous plastic material.
Claim 27
Dependent Claim
45 words
The diagnostic method according to claim 16, wherein the diamino-biphenyl derivative is tolidine in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms.
Claim 28
Dependent Claim
45 words
The diagnostic method according to claim 16, wherein the diamino-biphenyl derivative is ortho-tolidine in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms.
Claim 29
Dependent Claim
14 words
The diagnostic method according to claim 16, wherein said instrument additionally contains a guaiac.
Claim 30
Dependent Claim
16 words
The diagnostic method according to claim 26, wherein the guaiac is alpha-guaiaconic acid or beta-guaiaconic acid.
Claim 31
Dependent Claim
18 words
The diagnostic method according to claim 16, wherein the agent for increasing the viscosity is a protective colloid.
Claim 32
Dependent Claim
18 words
The diagnostic method according to claim 28, wherein the protective colloid is a polyalkylene glycol or polyvinyl alcohol.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-01-01 | 1982-07-03 | 1983-01-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-01-01 | 1986-07-03 | 1987-01-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-01-01 | 1990-07-03 | 1991-01-02 | $7700.00 | Window Closed / Lapsed |