US4130470A
Expired / Lapsed
US
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Method for generating a pH-function for use in electrophoresis
Assignee: Aminkemi AB
•
Filing: 1976-12-07
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Granted: 1978-12-19
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Expires: 1996-12-07
Abstract
Abstract Method for changing the pH value of a compound used in electrophoresis which comprises the generating of the pH-function along at least part of the separation path from the protolysis equilibrium with the separation method and/or chargeable groups immobilized in a matrix, in which the groups contain one or more of the elements carbon, sulphur, phosphorus, boron, or nitrogen.
Inventors
- 👤 Ake Rosengren
- 👤 Bengt Bjellqvist
- 👤 Vesna Gasparic
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 01:27
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44795; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
98 words
Method for generating a pH function for use in electrophoresis, characterized in that the pH function at least along parts of the separation path is generated from the protolysis equilibriums with the separation medium of charged and/or chargeable groups immobilized to vary the pH function in a matrix along parts of the separation path with chemically affixed groups of various concentrations along said path to thereby prevent transport of said groups with respect to the matrix upon application of an outer electrical field, said groups containing one or several of the elements C, S, P, B or N.
Claim 2
Dependent Claim
98 words
Method for generating a pH function for use in electrophoresis, characterized in that the pH function at least along parts of the separation path is generated from the protolysis equilibriums with the separation medium of charged and/or chargeable groups immobilized to vary the pH function in a matrix along parts of the separation path with chemically affixed groups of various concentrations along said path to thereby prevent transport of said groups with respect to the matrix upon application of an outer electrical field, said groups containing one or several of the elements C, S, P, B or N.
Claim 3
Dependent Claim
32 words
Method according to claim 1, characterized in that the pH gradient is alternatively generated by the protolysis equilibrium with the separation medium of immobilized and not immobilized charged and/or chargeable functional groups.
Claim 4
Dependent Claim
27 words
Method according to claim 1, characterized in that the pH gradient could be monotonous or not monotonous within the pH interval 2-12 or parts of this range.
Claim 5
Dependent Claim
15 words
Method according to claim 1, characterized in that the matrix constitutes the convection stabilizing medium.
Claim 6
Dependent Claim
15 words
Method according to claim 4, characterized in that the matrix is formed by a gel.
Claim 7
Dependent Claim
14 words
Method according to claim 5, characterized in that the gel is a porosity gradient.
Claim 8
Dependent Claim
12 words
Method according to claim 4, characterized in that the matrix is granular.
Claim 9
Dependent Claim
12 words
Method according to claim 4, characterized in that the matrix is fibrous.
Claim 10
Dependent Claim
20 words
Method according to claim 1, characterized in that the matrix constitutes one or several surfaces in a capillary stabilized system.
Claim 11
Dependent Claim
17 words
Method according to claim 1, characterized in that the electrophoretic separation is made as a zone electrophoresis.
Claim 12
Dependent Claim
16 words
Method according to claim 1, characterized in that the electrophoretic separation is performed as an immunoelectrophoresis.
Claim 13
Dependent Claim
17 words
Method according to claim 1, characterized in that the electrophoretic separation is carried out as an electrofocusing.
Claim 14
Dependent Claim
22 words
Method according to claim 12, characterized in that the electrofocusing is performed in the presence of one or several not immobilized ampholines.
Claim 15
Dependent Claim
34 words
Method according to claim 2, characterized in that the matrix carrying the immobilized groups constitutes permeable membranes in a chamber electrolyser and that the pH function is general alternatively of these membranes and ampholytes.
References Cited (Backward Citations)
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US3640809A
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US3704217A
Cited
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Cited
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Cited
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-12-18 | 1982-06-19 | 1982-12-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-12-18 | 1986-06-19 | 1986-12-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-12-18 | 1990-06-19 | 1990-12-19 | $7700.00 | Window Closed / Lapsed |