US4126671A
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Method for detecting bovine leukemia viral infection
Assignee: INTERNATONAL MINERALS & CHEMICAL Corp A CORP OF NY
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Filing: 1976-08-17
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Granted: 1978-11-21
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Expires: 1996-08-17
Abstract
Abstract A new immunodiffusion test method for determining the presence of bovine leukemia viral infection in cattle. The method relies upon the formation of a precipitin line resulting from the reaction of glycoprotein antigen with an antibody. The reaction is conducted in a liquid gel medium. By comparing the precipitin line of this test against a standard the presence of bovine leukemia infection can be diagnosed. Also covered is the immunological composition comprising said antigen and said liquid gel media.
Inventors
- 👤 James A. House
- 👤 Carol House
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 15:31
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C07K16/00; C07K16/08; C07K16/10; C07K16/112; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/558; G01N33/559; G01N33/569; G01N33/56983; A; A61; A61K; A61K39/00; C12; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/14011; C12N2740/14022; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S436/00; Y10S436/811; Y10S530/00; Y10S530/806; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/817; Y10S530/82; Y10S530/826; Y10S530/827; Y10S530/828
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
68 words
An immunological composition for detecting bovine leukemia virus infection in a bovine animal comprising: (1) an ether-labile antigen extracted from cell cultures infected with bovine leukemia virus; in homogeneous admixture with (2) a semi-solid gel medium; said composition being in the form of a thin layer and having at least one well therein adapted to receive a test specimen of an animal suspected of infection by said virus.
Claim 2
Dependent Claim
68 words
An immunological composition for detecting bovine leukemia virus infection in a bovine animal comprising: (1) an ether-labile antigen extracted from cell cultures infected with bovine leukemia virus; in homogeneous admixture with (2) a semi-solid gel medium; said composition being in the form of a thin layer and having at least one well therein adapted to receive a test specimen of an animal suspected of infection by said virus.
Claim 3
Dependent Claim
22 words
The immunological composition of claim 1 wherein the antigen is extracted from mammalian or avian cell cultures infected with bovine leukemia virus.
Claim 4
Dependent Claim
100 words
An immunological composition according to claim 1 comprising: (1) an ether-labile antigen extracted from mammalian cell cultures infected with bovine leukemia virus, said antigen being substantially free from contamination with specific antibodies so that upon contact with an antibody against the bovine leukemia virus it forms a precipitate to indicate the presence of said virus in the test animal; in homogeneous admixture with (2) a semi-solid gel medium; said composition being in the form of a thin layer and having at least one well therein adapted to receive a test specimen of an animal suspected of infection by said virus.
Claim 5
Dependent Claim
21 words
The immunological composition of claim 3 in which the antigen is extracted from a mammalian organ infected with bovine leukemia virus.
Claim 6
Dependent Claim
75 words
An immunological composition according to claim 1 comprising: (1) an ether-labile antigen extracted from fetal lamb spleen cell cultures infected with bovine leukemia virus; in homogeneous admixture with (2) an agar medium including borate buffer to pH3-10.5 and 3.0-120g per liter of sodium chloride; said composition being in the form of a thin layer having at least one well therein adapted to receive a test specimen of an animal suspected of infection by said virus.
Claim 7
Independent Claim
112 words
A method for determining the presence of antibodies to bovine leukemia virus in a bovine which comprises: (1) taking a blood serum sample from said bovine; (2) diffusing the said sample in a gel medium which contains a soluble ether-labile antigen extracted from cell cultures infected with bovine leukemia virus so that upon contact with an antibody to bovine leukemia virus it forms a precipitate or exerts an influence on the precipitin line to indicate the presence of said virus in the test animal; (3) observing the presence of the precipitin line which forms when said antibody comes into contact with said antigen or, alternatively, the absence of such a precipitin line.
Claim 8
Independent Claim
112 words
A method for determining the presence of antibodies to bovine leukemia virus in a bovine which comprises: (1) taking a blood serum sample from said bovine; (2) diffusing the said sample in a gel medium which contains a soluble ether-labile antigen extracted from cell cultures infected with bovine leukemia virus so that upon contact with an antibody to bovine leukemia virus it forms a precipitate or exerts an influence on the precipitin line to indicate the presence of said virus in the test animal; (3) observing the presence of the precipitin line which forms when said antibody comes into contact with said antigen or, alternatively, the absence of such a precipitin line.
Claim 9
Independent Claim
165 words
A method for determining the presence of antibodies to bovine leukemia virus in a bovine which comprises: (1) taking a blood serum sample from said bovine; (2) diffusing the said sample in a gel medium which contains a soluble ether-labile antigen extracted from cell cultures infected with bovine leukemia virus; said antigen being substantially free from contamination with specific antibodies so that upon contact with an antibody against bovine leukemia virus it forms a precipitate, or exerts an influence on the precipitin line to indicate the presence of antibodies to said virus in the test animal; (3) observing the presence of the precipitin line which forms when said antibody comes into contact with said antigen or, observing the absence of such a precipitin line; or, alternatively, observing the influence exerted on the precipitin line as an indication of the presence of antibodies to said virus in the test animal; and (4) comparing the observation (3) against a model in agar gel medium using reference serums.
Claim 10
Independent Claim
165 words
A method for determining the presence of antibodies to bovine leukemia virus in a bovine which comprises: (1) taking a blood serum sample from said bovine; (2) diffusing the said sample in a gel medium which contains a soluble ether-labile antigen extracted from cell cultures infected with bovine leukemia virus; said antigen being substantially free from contamination with specific antibodies so that upon contact with an antibody against bovine leukemia virus it forms a precipitate, or exerts an influence on the precipitin line to indicate the presence of antibodies to said virus in the test animal; (3) observing the presence of the precipitin line which forms when said antibody comes into contact with said antigen or, observing the absence of such a precipitin line; or, alternatively, observing the influence exerted on the precipitin line as an indication of the presence of antibodies to said virus in the test animal; and (4) comparing the observation (3) against a model in agar gel medium using reference serums.
Claim 11
Dependent Claim
29 words
The method according to claim 7 in which the antigen is extracted from a mammalian organ infected with bovine leukemia virus or is obtained from a lymphocyte suspension culture.
Claim 12
Dependent Claim
22 words
The method of claim 6 in which the antigen is extracted from mammalian or avian cell cultures infected with bovine leukemia virus.
Claim 13
Dependent Claim
20 words
The method of claim 6 in which the antigen is extracted from mammalian cell cultures infected with bovine leukemia virus.
Claim 14
Dependent Claim
22 words
The method of claim 6 in which the antigen is extracted from fetal lamb spleen cell cultures infected with bovine leukemia virus.
Claim 15
Dependent Claim
27 words
The method according to claim 6 in which the gel medium contains a concentration of sodium chloride in the range of from about 3.0-120 grams per liter.
Claim 16
Dependent Claim
20 words
The method of claim 6 in which the gel medium has a pH within the range of from about 3-10.5.
Claim 17
Independent Claim
120 words
A method of diagnosing the presence of bovine leukemia virus infection in a suspect bovine animal which comprises: (1) placing in separate wells on an immunodiffusion plate; (a) an ether-labile antigen for said virus which is extracted from cell cultures infected with bovine leukemia virus and having sufficient strength to form a distinct, specific precipitin line with the antiserum(b); (b) an antiserum for said virus which provides a specific distinct precipitin line with the antigen(a); and (c) the test serum of a suspect bovine animal; (2) observing the response thereto, a positive response being indicated by a precipitin line of identity or the influence which the test serum exerts on the line formed by the reagent serum and the antigen.
Claim 18
Independent Claim
120 words
A method of diagnosing the presence of bovine leukemia virus infection in a suspect bovine animal which comprises: (1) placing in separate wells on an immunodiffusion plate; (a) an ether-labile antigen for said virus which is extracted from cell cultures infected with bovine leukemia virus and having sufficient strength to form a distinct, specific precipitin line with the antiserum(b); (b) an antiserum for said virus which provides a specific distinct precipitin line with the antigen(a); and (c) the test serum of a suspect bovine animal; (2) observing the response thereto, a positive response being indicated by a precipitin line of identity or the influence which the test serum exerts on the line formed by the reagent serum and the antigen.
Claim 19
Dependent Claim
32 words
A method according to claim 14 in which the antigen for said virus is extracted from a mammalian organ infected with bovine leukemia virus or is obtained from a lymphocyte suspension culture.
Claim 20
Dependent Claim
19 words
The method of claim 14 in which the antigen is extracted from mammalian cell cultures or avian cell cultures.
Claim 21
Dependent Claim
20 words
The method of claim 14 in which the antigen is extracted from mammalian cell cultures infected with bovine leukemia virus.
Claim 22
Dependent Claim
22 words
The method of claim 14 in which the antigen is extracted from fetal lamb spleen cell cultures infected with bovine leukemia virus.
Claim 23
Dependent Claim
27 words
The method according to claim 10 in which the gel medium contains a concentration of sodium chloride in the range of from about 3.0-120 grams per liter.
Claim 24
Dependent Claim
20 words
The method of claim 10 in which the gel medium has a pH in the range of from about 3-10.5.
Claim 25
Independent Claim
106 words
A method for detecting antibodies to bovine leukemia virus in a bovine which comprises: (1) placing a test serum into at least one well in an indicator system comprising; (a) a gel medium having homogeneously mixed therein an ether-labile antigen extracted from cell cultures infected with bovine leukemia virus; and (2) determining the presence of a precipitate, a positive test being in the form of a visible precipitate which results from the combination of said antigen in the gel medium(a) and the antibody of the test serum or the influence which the test serum exerts on the line formed by a reagent serum and the antigen.
Claim 26
Independent Claim
106 words
A method for detecting antibodies to bovine leukemia virus in a bovine which comprises: (1) placing a test serum into at least one well in an indicator system comprising; (a) a gel medium having homogeneously mixed therein an ether-labile antigen extracted from cell cultures infected with bovine leukemia virus; and (2) determining the presence of a precipitate, a positive test being in the form of a visible precipitate which results from the combination of said antigen in the gel medium(a) and the antibody of the test serum or the influence which the test serum exerts on the line formed by a reagent serum and the antigen.
Claim 27
Dependent Claim
28 words
A method according to claim 21 in which the antigen is extracted from mammalian organs infected with bovine leukemia virus or is obtained from a lymphocyte suspension culture.
Claim 28
Dependent Claim
19 words
The method of claim 16 in which the antigen is extracted from mammalian cell cultures or avian cell cultures.
Claim 29
Dependent Claim
20 words
The method of claim 21 in which the antigen is extracted from mammalian cell cultures infected with bovine leukemia virus.
Claim 30
Dependent Claim
22 words
The method of claim 21 in which the antigen is extracted from fetal lamb spleen cell cultures infected with bovine leukemia virus.
Claim 31
Dependent Claim
27 words
The method according to claim 16 in which the gel medium contains a concentration of sodium chloride in the range of from about 3.0-120 grams per liter.
Claim 32
Dependent Claim
20 words
The method of claim 16 in which the gel medium has a pH in the range of from about 3-10.5.
Claim 33
Independent Claim
128 words
A method for determining the presence of antibodies to bovine leukemia virus in a bovine which comprises: (1) taking a blood serum sample from said bovine; (2) diffusing the said sample in a gel medium which contains a soluble ether-labile antigen extracted from cell cultures infected with bovine leukemia virus so that upon contact with an antibody to bovine leukemia virus it forms a precipitate or exerts an influence on the precipitin line to indicate the presence of said virus in the test animal; (3) observing the presence of the precipitin line which forms when said antibody comes into contact with said antigen or, alternatively, the absence of such a precipitin line; and (4) comparing the observation (3) against a model in agar gel medium using reference serum.
Claim 34
Independent Claim
128 words
A method for determining the presence of antibodies to bovine leukemia virus in a bovine which comprises: (1) taking a blood serum sample from said bovine; (2) diffusing the said sample in a gel medium which contains a soluble ether-labile antigen extracted from cell cultures infected with bovine leukemia virus so that upon contact with an antibody to bovine leukemia virus it forms a precipitate or exerts an influence on the precipitin line to indicate the presence of said virus in the test animal; (3) observing the presence of the precipitin line which forms when said antibody comes into contact with said antigen or, alternatively, the absence of such a precipitin line; and (4) comparing the observation (3) against a model in agar gel medium using reference serum.
Claim 35
Independent Claim
147 words
A method of diagnosing the presence of bovine leukemia virus infection in a suspect bovine animal which comprises: (1) placing in separate wells on an immunodiffusion plate: (a) an ether-labile antigen for said virus which is extracted from cell cultures infected with bovine leukemia virus and having sufficient strength to form a distinct, specific precipitin line with the antiserum (b); (b) an antiserum for said virus which provides a specific distinct precipitin line with th antigen(a); and (c) the test serum of a suspect bovine animal; (2) observing the response thereto, a positive response being indicated by a precipitin line of identity or the influence which the test serum exerts on the line formed by the reagent serum and the antigen; and (3) comparing the observation (2) against a model on a similar immunodiffusion plate comprising the antigen, an antiserum and the reference sera of known strength.
Claim 36
Independent Claim
147 words
A method of diagnosing the presence of bovine leukemia virus infection in a suspect bovine animal which comprises: (1) placing in separate wells on an immunodiffusion plate: (a) an ether-labile antigen for said virus which is extracted from cell cultures infected with bovine leukemia virus and having sufficient strength to form a distinct, specific precipitin line with the antiserum (b); (b) an antiserum for said virus which provides a specific distinct precipitin line with th antigen(a); and (c) the test serum of a suspect bovine animal; (2) observing the response thereto, a positive response being indicated by a precipitin line of identity or the influence which the test serum exerts on the line formed by the reagent serum and the antigen; and (3) comparing the observation (2) against a model on a similar immunodiffusion plate comprising the antigen, an antiserum and the reference sera of known strength.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-11-20 | 1982-05-22 | 1982-11-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-11-20 | 1986-05-22 | 1986-11-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-11-20 | 1990-05-22 | 1990-11-21 | $7700.00 | Window Closed / Lapsed |