US4126628A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Acylation of amino acids
Assignee: HER MAJESTY IN RIGHT OF CANADA AGRICULTURE, Minister of
•
Filing: 1977-03-28
•
Granted: 1978-11-21
•
Expires: 1997-03-28
Abstract
Abstract N-mono-substituted derivatives of diamino acids are prepared by the reaction of succinimidyl esters of carboxylic acids or substituted carbonic acids with the unprotected diamino acid. The acylation preferentially occurs at the side chain or terminal amino group of the diamino acid. For example, selective acylation of the terminal amino group of lysine occurs without first having protected the 2-amino group. Such acylation has application in the preparation of inter alia N 6 -palmitoyl-lysine.
Inventors
- 👤 Alenka M. Paquet
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 10:47
CPC Classifications
C; C07; C07D; C07D207/00; C07D207/02; C07D207/30; C07D207/34; C07D207/36; C07D207/40; C07D207/404; C07D207/46
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
137 words
A method for monoacylation of diamino acids, the acyl group being selected from carboxylic acid and substituted carbonic acid moieties, the acylation occurring preferentially at the terminal amino group remote from the carboxyl group of the amino acid, comprising (a) preparing the succinimidyl ester of the selected carboxylic or alkyl or aralkyl carbonic acid; (b) reacting, in the presence of a basic catalyst to give a pH of at least about 10 or equivalent high basicity, said succinimidyl ester with the diamino acid, its ester or oligopeptide thereof the amount of basic catalyst or pH being high enough to form the amide linkage between the terminal amino group and the acid without significant acylation of the other unprotected amino group, in an inert liquid medium; and (c) recovering the N-acyldiamino acid, ester or oligopeptide thereof as product.
Claim 2
Independent Claim
137 words
A method for monoacylation of diamino acids, the acyl group being selected from carboxylic acid and substituted carbonic acid moieties, the acylation occurring preferentially at the terminal amino group remote from the carboxyl group of the amino acid, comprising (a) preparing the succinimidyl ester of the selected carboxylic or alkyl or aralkyl carbonic acid; (b) reacting, in the presence of a basic catalyst to give a pH of at least about 10 or equivalent high basicity, said succinimidyl ester with the diamino acid, its ester or oligopeptide thereof the amount of basic catalyst or pH being high enough to form the amide linkage between the terminal amino group and the acid without significant acylation of the other unprotected amino group, in an inert liquid medium; and (c) recovering the N-acyldiamino acid, ester or oligopeptide thereof as product.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the diamino acid is selected from the group consisting of lysine, ornithine and α,γ-diaminobutyric acid.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein the acylating ester comprises fatty acyl groups having at least about 6 carbon atoms.
Claim 5
Dependent Claim
23 words
The method of claim 1 wherein the acylating ester comprises alkyl oxycarbonyl group suitable as a protecting group for the terminal amino group.
Claim 6
Dependent Claim
17 words
The method of claim 1 wherein the pH in step (b) is from about 10 to 12.5.
Claim 7
Dependent Claim
35 words
The method of claim 1 wherein in step (a) thereof, N-hydroxysuccinimide in the form of its thallium salt is reacted with selected acyl halide, the thallium halide precipitate separated, and the N-succinimidyl acid ester recovered.
Claim 8
Dependent Claim
38 words
The method of claim 1 wherein in step (b) thereof, said reaction takes place at about room temperature in the presence of trialkylamine as catalyst in amounts of at least about 2 equivalents based on the diamino acid.
Claim 9
Dependent Claim
25 words
The method of claim 1 wherein in step (c) thereof, the acylated amino acid is recovered by precipitation, filtration or centrifugation, washing and drying thereof.
Claim 10
Dependent Claim
22 words
The method of claim 1 wherein after step (b) thereof, N-hydroxysuccinimide remains in the reaction medium and is recycled to step (a).
Claim 11
Dependent Claim
27 words
The method of claim 1 wherein the amino acid is lysine and the acyl group is derived from fatty acids having from 8 to 18 carbon atoms.
Claim 12
Dependent Claim
119 words
The method of claim 1 wherein (a) the thallium salt of N-hydroxysuccinimide in an inert solvent medium is reacted with acylchloride in approximately equimolar amounts, the acyl chloride being selected from fatty acid chlorides and alkylcarbonic acid chlorides, to give the succinimidyl ester, and the thallium chloride precipitate separated; (b) said succinimidyl ester is then reacted in an inert solvent medium with lysine, lysine hydrochloride, or lysine ester, in the presence of basic catalyst or alkaline catalyst to give a pH of about 10-12 or equivalent high basicity, the pH subsequently reduced or basic catalyst removed to precipitate reaction product; and (c) N-acyllysine or -lysine ester precipitate recovered, the acylation preferentially occurring on the terminal amino group of lysine.
Claim 13
Dependent Claim
34 words
The method of claim 11 wherein the basic or alkaline catalyst is triethylamine, which is subsequently removed, after the reaction in step (b), by evaporation under reduced pressure or is neutralized by mineral acid.
References Cited (Backward Citations)
US3317559A
Cited
US3541084A
Cited
US3880823A
Cited
US3578641A
Cited
US4043992A
Cited
US3780015A
Cited
US3870694A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-11-20 | 1982-05-22 | 1982-11-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-11-20 | 1986-05-22 | 1986-11-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-11-20 | 1990-05-22 | 1990-11-21 | $7700.00 | Window Closed / Lapsed |