US4124700A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassay for thymopoietin
Assignee: Memorial Sloan Kettering Cancer Center
•
Filing: 1977-06-27
•
Granted: 1978-11-07
•
Expires: 1997-06-27
Abstract
Abstract An immunoassay for the polypeptide thymic hormone thymopoietin is described. Determination of thymopoietin levels in biological fluids provides a useful diagnostic test for myasthenia gravis (elevated levels), immune deficiency diseases (reduced levels), (immunologically mediated diseases such as rheumatoid arthritis, systemic lupus erythematosus and allergy (reduced levels), cancer (reduced levels), malnutrition (reduced levels) and infections (reduced levels). The present assay is also useful as a monitor of the effectiveness of therapy in each of the aforementioned types of disease states.
Inventors
- 👤 Gideon Goldstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:08
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/813; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
12 words
An antigen consisting essentially of thymopoietin bonded to an immunogenic carrier material.
Claim 2
Dependent Claim
12 words
An antigen consisting essentially of thymopoietin bonded to an immunogenic carrier material.
Claim 3
Dependent Claim
19 words
An antigen according to claim 1 wherein bonding of said thymopoietin to said carrier material is by covalent bonding.
Claim 4
Dependent Claim
13 words
The antigen of claim 1 wherein said immunogenic carrier material is a protein.
Claim 5
Dependent Claim
13 words
The antigen of claim 3 wherein said protein is a mammalian gamma globulin.
Claim 6
Dependent Claim
12 words
The antigen of claim 3 wherein said protein is bovine serum albumin.
Claim 7
Dependent Claim
42 words
An antibody specific to thymopoietin and to an antigen consisting essentially of thymopoietin bonded to an immunogenic carrier material, said thymopoietin specific antibody being prepared by innoculating a host animal with the aforesaid antigen and collecting the serum from said host animal.
Claim 8
Dependent Claim
42 words
An antibody specific to thymopoietin and to an antigen consisting essentially of thymopoietin bonded to an immunogenic carrier material, said thymopoietin specific antibody being prepared by innoculating a host animal with the aforesaid antigen and collecting the serum from said host animal.
Claim 9
Dependent Claim
16 words
The antibody of claim 6 wherein said antigen consists of thymopoietin covalently bonded to a protein.
Claim 10
Dependent Claim
24 words
The antibody of claim 7 wherein said protein is bovine serum albumin and said covalent bonding is with glutaraldehyde as a bifunctional linking group.
Claim 11
Dependent Claim
8 words
Labeled thymopoietin wherein the label is iodine 131.
Claim 12
Dependent Claim
8 words
Labeled thymopoietin wherein the label is iodine 131.
Claim 13
Independent Claim
102 words
A method for the radioimmunoassay of thymopoietin in a serum sample, which method comprises mixing said sample with a known amount of radiolabeled thymopoietin and an antibody which will selectively complex with said thymopoietin, separating the resulting antibody-antigen complex from the supernatant, measuring the degree of binding of said radiolabeled thymopoietin in said complex and determining the amount of thymopoientin present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of thymopoietin with fixed amounts of labeled thymopoietin and said antibody, and determining the degree of binding for each known amount of thymopoietin.
Claim 14
Independent Claim
102 words
A method for the radioimmunoassay of thymopoietin in a serum sample, which method comprises mixing said sample with a known amount of radiolabeled thymopoietin and an antibody which will selectively complex with said thymopoietin, separating the resulting antibody-antigen complex from the supernatant, measuring the degree of binding of said radiolabeled thymopoietin in said complex and determining the amount of thymopoientin present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of thymopoietin with fixed amounts of labeled thymopoietin and said antibody, and determining the degree of binding for each known amount of thymopoietin.
References Cited (Backward Citations)
US4055633A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-11-06 | 1982-05-08 | 1982-11-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-11-06 | 1986-05-08 | 1986-11-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-11-06 | 1990-05-08 | 1990-11-07 | $7700.00 | Window Closed / Lapsed |