US4119494A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of enzymes in an anhydrous medium
Assignee: Rhone Poulenc Industries SA
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Filing: 1976-12-20
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Granted: 1978-10-10
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Expires: 1996-12-20
Abstract
Abstract Fixing of enzymes to a mineral, organic or organomineral support is carried out in an anhydrous organic liquid medium at a temperature above 60° C up to 120° C. Fixation in an anhydrous liquid medium permits obtaining support - enzyme complexes with a fast fixation reaction speed at high temperatures and without any particular pH value condition.
Inventors
- 👤 Gilbert Durand
- 👤 Pierre Monsan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:32
CPC Classifications
C; C12; C12N; C12N11/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
60 words
Process for fixing enzymes on supports comprising providing a solid support having groups which react with the enzymes to fix the enzyme to the support, reacting the enzyme with the support at a temperature within the range of more than 60° C. up to 120° C. in an anhydrous organic liquid in which both the enzyme and support are insoluble.
Claim 2
Dependent Claim
60 words
Process for fixing enzymes on supports comprising providing a solid support having groups which react with the enzymes to fix the enzyme to the support, reacting the enzyme with the support at a temperature within the range of more than 60° C. up to 120° C. in an anhydrous organic liquid in which both the enzyme and support are insoluble.
Claim 3
Dependent Claim
27 words
Process as claimed in claim 1 in which the enzymes to be fixed are selected from the group consisting of oxydoreductases, transferases, hydrolases, lyases, isomerases, and ligases.
Claim 4
Dependent Claim
25 words
Process as claimed in claim 1 in which the support is a material selected from the group consisting of a mineral, organic or organomineral material.
Claim 5
Dependent Claim
58 words
Process as claimed in claim 3 in which the support is selected from the group consisting of brick, silica, alumina, clay, sand; a natural organic polymer selected from the group consisting of agarose, starch, polydextran, cellulose; or a synthetic organic polymer selected from the group consisting of polybutadiene, polystyrene, copolymers of methacrylic acid and copolymers of maleic anhydride-ethylene.
Claim 6
Dependent Claim
14 words
Process as claimed in claim 1 in which the support is an activated support.
Claim 7
Dependent Claim
34 words
Process as claimed in claim 1 in which the organic liquid medium is selected from the group consisting of unsubstituted and chlorosubstituted aliphatic, cycloaliphatic and aromatic hydrocarbons, with or without oxygen and/or sulphur atoms.
Claim 8
Dependent Claim
16 words
Process as claimed in claim 1 in which the reaction is carried out under slight pressure.
Claim 9
Dependent Claim
32 words
Process as claimed in claim 1 in which the reaction for fixing the enzyme onto the support is carried out for a time within the range of several minutes to 2 hours.
Claim 10
Dependent Claim
33 words
Process as claimed in claim 1 in which the organic liquid medium is selected from the group consisting of hexane, benzene, toluene, xylene, chloroform, carbontetrachloride, dichloroethane, trichloroethane, trichloroethylene, perchloroethylene, dioxane, tetrahydrofuran, and dimethylsulphoxide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-09 | 1982-04-10 | 1982-10-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-09 | 1986-04-10 | 1986-10-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-09 | 1990-04-10 | 1990-10-10 | $7700.00 | Window Closed / Lapsed |