US4117113A
Expired / Lapsed
US
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Immunological preparations
Assignee: National Research Development Corp UK
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Filing: 1977-08-22
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Granted: 1978-09-26
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Expires: 1997-08-22
Abstract
Abstract Immunological preparations are described in which liposomes having negative charge are used as adjuvants for the purposes of human and veterinary vaccines containing viral antigens. The formulation of influenza antigen in liposomes formed with egg lecithin is described and the adjuvant effect demonstrated.
Inventors
- 👤 Anthony Clifford Allison
- 👤 Gregory Gregoriadis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:32
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/145; A61K39/39; A61K9/00; A61K9/10; A61K9/127; C; C12; C12N; C12N7/00; A61K2039/555; A61K2039/55511; A61K2039/55555; A61K2039/55572; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16134; C12N2760/16151; C12N2760/16211; C12N2760/16251; C12N2760/16311; C12N2760/16351; Y; Y10; Y10S; Y10S424/00; Y10S424/812
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
33 words
A pharmaceutical preparation for administration in vivo to effect immunization comprising an immunologically effective agent selected from viral antigens which agent is incorporated essentially entrapped in liposomes, said liposomes having a negative charge.
Claim 2
Dependent Claim
33 words
A pharmaceutical preparation for administration in vivo to effect immunization comprising an immunologically effective agent selected from viral antigens which agent is incorporated essentially entrapped in liposomes, said liposomes having a negative charge.
Claim 3
Dependent Claim
15 words
A pharmaceutical preparation according to claim 1, formulated as dosage units for use in humans.
Claim 4
Dependent Claim
14 words
A pharmaceutical preparation according to claim 1, wherein the virus is an influenza virus.
Claim 5
Dependent Claim
15 words
A pharmaceutical preparation according to claim 1, wherein the liposomes are formed with egg lecithin.
Claim 6
Dependent Claim
30 words
A pharmaceutical preparation according to claim 1, in which the negative charge is due to the presence of a substance selected from phosphatidic acid, dicetyl phosphate and beef brain ganglioside.
Claim 7
Independent Claim
42 words
A method for the immunization of humans and animals against viral infections which comprises administering to such humans and animals an immunologically effective agent selected from viral antigens which agent is incorporated essentially entrapped in liposomes, said liposomes having a negative charge.
Claim 8
Independent Claim
42 words
A method for the immunization of humans and animals against viral infections which comprises administering to such humans and animals an immunologically effective agent selected from viral antigens which agent is incorporated essentially entrapped in liposomes, said liposomes having a negative charge.
Claim 9
Dependent Claim
20 words
A method according to claim 6, which comprises administering said immunologically effective agent to a human in unit dosage form.
Claim 10
Dependent Claim
13 words
A method according to claim 7, wherein the virus is an influenza virus.
Claim 11
Dependent Claim
15 words
A method according to claim 6, wherein the immunologically effective agent is administered by injection.
Claim 12
Dependent Claim
54 words
A pharmaceutical method which comprises mixing a viral antigen with a lipid, water and a substance capable of imparting a negative charge, sonicating this mixture to produce liposomes having a negative charge with which said viral antigen is incorporated essentially entrapped, and administering said liposomes to a human or animal to effect immunization thereof.
Claim 13
Dependent Claim
54 words
A pharmaceutical method which comprises mixing a viral antigen with a lipid, water and a substance capable of imparting a negative charge, sonicating this mixture to produce liposomes having a negative charge with which said viral antigen is incorporated essentially entrapped, and administering said liposomes to a human or animal to effect immunization thereof.
Claim 14
Dependent Claim
12 words
A method according to claim 10, wherein the lipid is egg lecithin.
Claim 15
Dependent Claim
26 words
A method according to claim 10, wherein the substance capable of imparting a negative charge is selected from phosphatidic acid, dicetyl phosphate and beef brain ganglioside.
Claim 16
Dependent Claim
33 words
In the art of parenterally administering viral antigens to patients susceptible to immunization therewith, the improvement which comprise administering such antigens essentially entrapped in negatively charged liposomes to enhance the immune response thereto.
Claim 17
Dependent Claim
33 words
In the art of parenterally administering viral antigens to patients susceptible to immunization therewith, the improvement which comprise administering such antigens essentially entrapped in negatively charged liposomes to enhance the immune response thereto.
Claim 18
Dependent Claim
15 words
The improvement of claim 13, wherein the viral antigen administered is an influenza virus antigen.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-09-25 | 1982-03-27 | 1982-09-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-09-25 | 1986-03-27 | 1986-09-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-09-25 | 1990-03-27 | 1990-09-26 | $7700.00 | Window Closed / Lapsed |