US4109075A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Separation of saccharides by exclusion chromatography
Assignee: Unilever Bestfoods North America
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Filing: 1976-06-09
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Granted: 1978-08-22
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Expires: 1996-06-09
Abstract
Abstract A process for separating saccharides by exclusion chromatography wherein a feed mixture of saccharides is supplied to a molecular exclusion chromatography zone from which at least three fractions are removed. The first fraction contains the least diffusible saccharides of the mixture which generally are the highest molecular weight components, the second fraction contains predominantly intermediate molecular weight saccharides and the third fraction contains predominantly lower molecular weight saccharide fractions. After supplying the feed to the column, the zone is continuously eluted, first with the first fraction removed from the column followed by elution with water. The use of the first fraction from the column as an eluting solvent serves to minimize the amount of water employed as an eluting solvent.
Inventors
- 👤 Irving F. Deaton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 05:25
CPC Classifications
C; C13; C13K; C13K13/00; C13K13/007; B; B01; B01D; B01D15/00; B01D15/08; B01D15/10; B01D15/18; B01D15/1814; B01D15/26; B01D15/34
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
108 words
A process for separating saccaharides by exculsion chromatography comprising the steps of a. supplying a feed mixture of saccharides to a molecular exclusion chromatography zone, b. collecting from the zone a first fraction containing the least diffusible saccharides of the mixture, said first fraction containing predominantly the highest molecular weight saccharides, c. collecting subsequent fractions of saccharides from the zone, including a second fraction containing predominantly intermediate molecular weight saccharides and a third fraction containing predominantly lower molecular weight saccharides, d. continuously eluting said zone, with the elution being carried out first with said first fraction and then with water, and e. recovering the fractions from the zone.
Claim 2
Dependent Claim
108 words
A process for separating saccaharides by exculsion chromatography comprising the steps of a. supplying a feed mixture of saccharides to a molecular exclusion chromatography zone, b. collecting from the zone a first fraction containing the least diffusible saccharides of the mixture, said first fraction containing predominantly the highest molecular weight saccharides, c. collecting subsequent fractions of saccharides from the zone, including a second fraction containing predominantly intermediate molecular weight saccharides and a third fraction containing predominantly lower molecular weight saccharides, d. continuously eluting said zone, with the elution being carried out first with said first fraction and then with water, and e. recovering the fractions from the zone.
Claim 3
Dependent Claim
25 words
A process as defined in claim 1 wherein the feed mixture is a starch conversion product containing monosaccharides, disaccharides, trisaccharides, tetrasacchraides, pentasaccharides and higher polysaccharides.
Claim 4
Dependent Claim
19 words
A process as defined in claim 1 wherein the feed mixture contains levulose, dextrose and disaccharides and higher saccharides.
Claim 5
Dependent Claim
125 words
A process for altering the saccharide distribution of polysaccharides by exclusion chromatography comprising the steps of: a. supplying a feed mixture of saccharides containing monosaccharides, disaccharides, trisaccharides, tetrasaccharides, pentasaccharides and higher polysaccharides to a molecular exclusion chromatography zone, b. collecting from the zone a first fraction containing the highest molecular weight, least diffusible saccharides of the mixture, c. collecting subsequent fractions of saccharides from the zone, including a second fraction containing predominantly intermediate molecular weight saccharides, and a third fraction containing predominantly lower molecular weight saccharides, d. continuously eluting said zone, with the elution being carried out first with said first fraction and then with water, and e. recoving the fractions having a saccharide distribution different from that of the feed mixture from the zone.
Claim 6
Dependent Claim
125 words
A process for altering the saccharide distribution of polysaccharides by exclusion chromatography comprising the steps of: a. supplying a feed mixture of saccharides containing monosaccharides, disaccharides, trisaccharides, tetrasaccharides, pentasaccharides and higher polysaccharides to a molecular exclusion chromatography zone, b. collecting from the zone a first fraction containing the highest molecular weight, least diffusible saccharides of the mixture, c. collecting subsequent fractions of saccharides from the zone, including a second fraction containing predominantly intermediate molecular weight saccharides, and a third fraction containing predominantly lower molecular weight saccharides, d. continuously eluting said zone, with the elution being carried out first with said first fraction and then with water, and e. recoving the fractions having a saccharide distribution different from that of the feed mixture from the zone.
Claim 7
Dependent Claim
101 words
A process for separating levulose from dextrose and polysaccharides by exclusion chromatography comprising the steps of a. supplying a feed mixture containing levulose, dextrose and polysaccharides to a molecular exclusion chromatography zone, b. collecting from the zone a first fraction containing the highest molecular weight, least diffusible saccharides of the mixture, c. collecting subsequent fractions of saccharides from the zone, including a second fraction rich in dextrose and a third fraction rich in levulose, d. continuously eluting said zone, with the elution being carried out with said first fraction and then with water, and e. recovering the fractions from the zone.
Claim 8
Dependent Claim
101 words
A process for separating levulose from dextrose and polysaccharides by exclusion chromatography comprising the steps of a. supplying a feed mixture containing levulose, dextrose and polysaccharides to a molecular exclusion chromatography zone, b. collecting from the zone a first fraction containing the highest molecular weight, least diffusible saccharides of the mixture, c. collecting subsequent fractions of saccharides from the zone, including a second fraction rich in dextrose and a third fraction rich in levulose, d. continuously eluting said zone, with the elution being carried out with said first fraction and then with water, and e. recovering the fractions from the zone.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-08-21 | 1982-02-20 | 1982-08-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-08-21 | 1986-02-20 | 1986-08-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-08-21 | 1990-02-20 | 1990-08-22 | $7700.00 | Window Closed / Lapsed |