US4094964A
Expired / Lapsed
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Clonidine assay
Assignee: Roche Diagnostics Corp
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Filing: 1977-05-10
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Granted: 1978-06-13
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Expires: 1997-05-10
Abstract
Abstract A sensitive immunoassay for clonidine, 2-(2,6-dichloroanilino)-2-imidazoline hydrochloride is described. To prepare the clonidine selective antiserum, an antigen is made comprising 4-[[6-[2,4-dichloro-3(4,5-dihydro-1H-imidazol-2-yl)amino]hydroxyphenyl]azo]benzoic acid covalently bonded to an immunogenic carrier material through a peptide bond formed from said carboxyl group and amino groups contained in said immunogenic carrier material and the antigen is injected into a suitable host animal to elicit the desired antiserum.
Inventors
- 👤 Bevyn Jarrott
- 👤 Sidney Spector
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 11:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; C; C07; C07D; C07D233/00; C07D233/04; C07D233/28; C07D233/44; C07D233/50; G01N33/536; G01N33/537; G01N33/539; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/817; Y10S436/823
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
37 words
An antigen consisting essentially of a hapten, 4-[[6-[2,4-dichloro-3-(4,5-dihydro-1H-imidazol-2-yl)amino]hydroxyphenyl]azo]benzoic acid and acid addition salts thereof, covalently bonded to an immunogenic carrier material through peptide bonds formed between said carboxyl group and amino groups on said immunogenic carrier material.
Claim 2
Dependent Claim
37 words
An antigen consisting essentially of a hapten, 4-[[6-[2,4-dichloro-3-(4,5-dihydro-1H-imidazol-2-yl)amino]hydroxyphenyl]azo]benzoic acid and acid addition salts thereof, covalently bonded to an immunogenic carrier material through peptide bonds formed between said carboxyl group and amino groups on said immunogenic carrier material.
Claim 3
Dependent Claim
14 words
The antigen of claim 1 wherein said immunogenic carrier material is bovine serum albumin.
Claim 4
Dependent Claim
11 words
The antigen of claim 1 wherein said hapten is 4-[[6-[2,4-dichloro-3(4,5-dihydro-1H-imidazol-2-yl)amino]hydroxyphenyl]azo]benzoic acid.
Claim 5
Dependent Claim
57 words
An antibody specific to clonidine prepared by innoculating a host animal with an antigen consisting essentially of a hapten, 4-[[6-[2,4-dichloro-3-(4,5-dihydro-1H-imidazol-2-yl)amino]hydroxyphenyl]azo]benozic acid and acid addition salts thereof, covalently bonded to an immunogenic carrier material through peptide bonds formed between said carboxyl group and amino groups on said immunogenic carrier material and collecting the serum from said host animal.
Claim 6
Dependent Claim
57 words
An antibody specific to clonidine prepared by innoculating a host animal with an antigen consisting essentially of a hapten, 4-[[6-[2,4-dichloro-3-(4,5-dihydro-1H-imidazol-2-yl)amino]hydroxyphenyl]azo]benozic acid and acid addition salts thereof, covalently bonded to an immunogenic carrier material through peptide bonds formed between said carboxyl group and amino groups on said immunogenic carrier material and collecting the serum from said host animal.
Claim 7
Dependent Claim
19 words
The antibody of claim 4 wherein said antigen consists essentially of 4-[[6-[2,4-dichloro-3-(4,5-dihydro-1H-imidazol-2-yl)amino]hydroxyphenyl]azo]benzoic acid covalently bonded to bovine serum albumin.
Claim 8
Independent Claim
94 words
A method for the assay of clonidine in a sample, which method comprises mixing said sample with a known amount of labelled clonidine and an antibody which will selectively complex with clonidine, measuring the degree of binding of said labelled clonidine compound with said antibody, and determining the amount of clonidine present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amount of clonidine with fixed amounts of said labelled clonidine and said antibody and determining the degree of binding for each known amount of clonidine.
Claim 9
Independent Claim
94 words
A method for the assay of clonidine in a sample, which method comprises mixing said sample with a known amount of labelled clonidine and an antibody which will selectively complex with clonidine, measuring the degree of binding of said labelled clonidine compound with said antibody, and determining the amount of clonidine present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amount of clonidine with fixed amounts of said labelled clonidine and said antibody and determining the degree of binding for each known amount of clonidine.
Claim 10
Dependent Claim
13 words
The method of claim 6 wherein said labelled clonidine is clonidine 14 C.
Claim 11
Dependent Claim
9 words
A hapten of the formula ##STR2## and salts thereof.
Claim 12
Dependent Claim
9 words
A hapten of the formula ##STR2## and salts thereof.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-06-12 | 1981-12-12 | 1982-06-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-06-12 | 1985-12-12 | 1986-06-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-06-12 | 1989-12-12 | 1990-06-13 | $7700.00 | Window Closed / Lapsed |