US4085203A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing vaccine
Assignee: Mallinckrodt Veterinary Inc
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Filing: 1976-03-25
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Granted: 1978-04-18
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Expires: 1996-03-25
Abstract
Abstract A cell culture system which comprises living eucaryotic cells, such as those of human or animal origin, or mycophyta dispersed within a solid carrier body or bed, which carrier consists of porous or particulate material capable of retaining the cells while allowing liquid media to pass through or to have contact with the said cells, and processes for preparing and maintaining such culture systems, particularly for the purpose of virus propagation.
Inventors
- 👤 Ronald Charles Telling
- 👤 Roy John Passingham
- 👤 Brian Lewis Kitchener
- 👤 David George Hopkinson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 01:48
CPC Classifications
C; C12; C12N; C12N7/00; C12N5/00; C12N5/0068; C12N5/0075; C12N2531/00; C12N2533/00; C12N2533/10; C12N2533/50; C12N2770/00; C12N2770/00011; C12N2770/32011; C12N2770/32111; C12N2770/32151
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
161 words
A process for preparing a vaccine which comprises: (a) preparing a carrier bed from a slurry of particulate material; (b) applying a liquid suspension of eucaryotic cells to the bed; (c) allowing the cells trapped within the internal cavities or spaces of the bed to grow; (d) circulating nutrient medium through the bed, whereby nutrients are delivered to the cells trapped within the internal cavities and metabolic and degradation products are removed in the liquid leaving the bed; (e) infecting the cells trapped within the internal cavities with a microorganism to which the cells are susceptible; (f) propagating the microorganism within the cells by circulating through the bed a medium suitable for growth of the microorganism; (g) collecting the medium leaving the bed containing the microorganisms which are released following disruption of the cells, whilst retaining cell debris within the bed; (h) inactivating the microorganism with an inactivating agent; and (i) adding the inactivated microorganism to a pharmaceutically acceptable carrier therefor.
Claim 2
Dependent Claim
161 words
A process for preparing a vaccine which comprises: (a) preparing a carrier bed from a slurry of particulate material; (b) applying a liquid suspension of eucaryotic cells to the bed; (c) allowing the cells trapped within the internal cavities or spaces of the bed to grow; (d) circulating nutrient medium through the bed, whereby nutrients are delivered to the cells trapped within the internal cavities and metabolic and degradation products are removed in the liquid leaving the bed; (e) infecting the cells trapped within the internal cavities with a microorganism to which the cells are susceptible; (f) propagating the microorganism within the cells by circulating through the bed a medium suitable for growth of the microorganism; (g) collecting the medium leaving the bed containing the microorganisms which are released following disruption of the cells, whilst retaining cell debris within the bed; (h) inactivating the microorganism with an inactivating agent; and (i) adding the inactivated microorganism to a pharmaceutically acceptable carrier therefor.
Claim 3
Dependent Claim
18 words
The process as claimed in claim 1, in which adjuvant is added to the inactivated microorganism and carrier.
Claim 4
Dependent Claim
17 words
A process as claimed in claim 2, in which the adjuvant is aluminium hydroxide combined with saponin.
Claim 5
Dependent Claim
17 words
The method of claim 1 in which the cells are eucaryotic cells of human or animal origin.
Claim 6
Dependent Claim
22 words
The method of claim 1 in which the particulate material has a particle retention size of between 0.1 μ and 2.00 μ.
Claim 7
Dependent Claim
13 words
The method of claim 1 in which the virus is a FMD virus.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-04-17 | 1981-10-17 | 1982-04-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-04-17 | 1985-10-17 | 1986-04-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-04-17 | 1989-10-17 | 1990-04-18 | $7700.00 | Window Closed / Lapsed |