Process for preparing functional yeast proteins using alkaline conditions

Claim 2

A process for preparing functional food yeast proteins comprising: (a) heating an aqueous slurry of whole yeast cells to an elevated temperature of from about 60° to about 100° C. to remove undesirable flavor and color bodies; (b) separating the yeast cells from the aqueous extract; (c) slurrying the separated yeast cells with a dilute alkaline solution having a pH of from about 8.5 to about 10.0 at a temperature of from about 85° to about 95° C. to remove nucleotidic materials; (d) separating the yeast cells from the alkaline extract; (e) reslurrying the separated yeast cells in a solution of about 0.1 to about 0.3 N sodium hydroxide or potassium hydroxide at a temperature of from about 85° to 100° C. to extract proteins; (f) neutralizing the slurry; (g) separating the undigested cell residue from the supernatant containing the extracted protein; (h) acidifying the supernatant to precipitate proteins at their isoelectric point; (i) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (j) neutralizing the mother liquor solution; and (k) drying the mother liquor solution to yield a yeast whippable protein.

Claim 3

The process of claim 1 wherein the precipitated proteins are neutralized and dried to yield a yeast protein isolate.

Claim 4

The process of claim 1 wherein the precipitated proteins are washed, neutralized, and dried to yield a yeast protein isolate.

Claim 5

The process of claim 1 wherein the separated cell residue of step (g) is washed with water which is combined with the supernatant prior to acidification.

Claim 6

The process of claim 1 wherein the mother liquor solution of step (k) is spray-dried.

Claim 7

The process of claim 1 wherein the supernatant of step (h) is acidified to a pH of from about 3.5 to 4.5.

Claim 8

The process of claim 1 wherein the separated yeast cells of step (e) are reslurried in a solution of 0.1 to 0.3 N sodium hydroxide.

Claim 9

The process of claim 1 wherein the food yeast is selected from the group consisting of Candida utilis, Saccharomyces cerevisiae, Saccharomyces fragilis, and Saccharomyces carlsbergensis.

Claim 10

A process for preparing functional food yeast proteins comprising: (a) heating an aqueous slurry of whole yeast cells to a temperature of from 60° to 100° C. to extract flavor and color bodies; (b) separating the yeast cells from the aqueous extract; (c) slurrying the separated yeast cells in a dilute alkaline solution having a pH of from about 8.5 to 10.0 at a temperature of from 85° to 95° C. to extract nucleotidic materials; (d) separating the yeast cells from the resulting alkaline extract; (e) reslurrying the separated yeast cells in a solution of from 0.1 to 0.3N sodium hydroxide at a temperature of from 85° to 100° C.; (f) neutralizing the slurry; (g) separating the undigested cell residue from the supernatant containing the extracted proteins; (h) acidifying the supernatant to a pH of from about 3.5 to 4.5 to precipitate proteins; (i) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (j) neutralizing the mother liquor solution to a pH of from 6.7 to 7.2; and (k) spray-drying the mother liquor solution to yield a yeast whipable protein.

Claim 11

A process for preparing functional food yeast proteins comprising: (a) heating an aqueous slurry of whole yeast cells to a temperature of from 60° to 100° C. to extract flavor and color bodies; (b) separating the yeast cells from the aqueous extract; (c) slurrying the separated yeast cells in a dilute alkaline solution having a pH of from about 8.5 to 10.0 at a temperature of from 85° to 95° C. to extract nucleotidic materials; (d) separating the yeast cells from the resulting alkaline extract; (e) reslurrying the separated yeast cells in a solution of from 0.1 to 0.3N sodium hydroxide at a temperature of from 85° to 100° C.; (f) neutralizing the slurry; (g) separating the undigested cell residue from the supernatant containing the extracted proteins; (h) acidifying the supernatant to a pH of from about 3.5 to 4.5 to precipitate proteins; (i) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (j) neutralizing the mother liquor solution to a pH of from 6.7 to 7.2; and (k) spray-drying the mother liquor solution to yield a yeast whipable protein.

Claim 12

The process of claim 9 wherein the precipitated proteins are dissolved in water by neutralization and spray-dried to yield a yeast protein isolate.

Claim 13

A process for preparing functional food yeast proteins comprising: (a) heating an aqueous slurry of from 10 to 14 weight percent (dry weight) of whole Candida utilis yeast cells to a temperature of about 95° C. for about from 1 to 5 minutes to remove flavor and color bodies; (b) separating the yeast cells from the aqueous extract; (c) slurrying the separated yeast cells in an alkaline solution having a pH of about 9.5 and a temperature of about 90° C. for from about 5 to 30 minutes to extract nucleotidic materials; (d) separating the yeast cells from the alkaline extract; (e) reslurrying the separated yeast cells in a 0.15N sodium hydroxide solution at a temperature of about 95° C. for from 15 to 45 minutes to extract proteins; (f) neutralizing the slurry; (g) separating the undigested cell residue from the supernatant containing the extracted proteins; (h) acidifying the supernatant to a pH of about 4.0 to precipitate proteins; (i) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (j) neutralizing the mother liquor solution to a pH of about 7.0; and (k) spray-drying the mother liquor solution to yield a yeast whippable protein.

Claim 14

A process for preparing functional food yeast proteins comprising: (a) heating an aqueous slurry of from 10 to 14 weight percent (dry weight) of whole Candida utilis yeast cells to a temperature of about 95° C. for about from 1 to 5 minutes to remove flavor and color bodies; (b) separating the yeast cells from the aqueous extract; (c) slurrying the separated yeast cells in an alkaline solution having a pH of about 9.5 and a temperature of about 90° C. for from about 5 to 30 minutes to extract nucleotidic materials; (d) separating the yeast cells from the alkaline extract; (e) reslurrying the separated yeast cells in a 0.15N sodium hydroxide solution at a temperature of about 95° C. for from 15 to 45 minutes to extract proteins; (f) neutralizing the slurry; (g) separating the undigested cell residue from the supernatant containing the extracted proteins; (h) acidifying the supernatant to a pH of about 4.0 to precipitate proteins; (i) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (j) neutralizing the mother liquor solution to a pH of about 7.0; and (k) spray-drying the mother liquor solution to yield a yeast whippable protein.

Claim 15

The process of claim 11 wherein the precipitated proteins are dissolved by neutralization in a solvent and spray-dried.

Claim 16

The process of claim 11 wherein the precipitated proteins are washed with water, dissolved in water by neutralization, and spray-dried.

Claim 17

The process of claim 11 wherein the precipitated proteins are washed with acetone, dissolved in water by neutralization, and spray-dried.

Claim 18

The process of claim 11 wherein the precipitated proteins are washed with ethanol, dissolved in water by neutralization, and spray-dried.

Claim 19

A process for preparing functional food yeast proteins comprising: (a) slurrying whole yeast cells in a dilute alkaline solution having a pH of from about 8.5 to about 10 at a temperature of from about 85° to about 95° C. to extract nucleotidic materials and undesirable flavor and color bodies; (b) separating the yeast cells from the alkaline extract; (c) reslurrying the separated yeast cells in a solution of about 0.1 to about 0.3 N sodium hydroxide or potassium hydroxide at a temperature of from about 85° to 100° C. to extract proteins; (d) neutralizing the slurry; (e) separating the undigested cell residue from the supernatant containing the extracted protein; (f) acidifying the supernatant to precipitate proteins; (g) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (h) neutralizing the mother liquor solution; and (i) drying the mother liquor solution to yield a yeast whippable protein.

Claim 20

A process for preparing functional food yeast proteins comprising: (a) slurrying whole yeast cells in a dilute alkaline solution having a pH of from about 8.5 to about 10 at a temperature of from about 85° to about 95° C. to extract nucleotidic materials and undesirable flavor and color bodies; (b) separating the yeast cells from the alkaline extract; (c) reslurrying the separated yeast cells in a solution of about 0.1 to about 0.3 N sodium hydroxide or potassium hydroxide at a temperature of from about 85° to 100° C. to extract proteins; (d) neutralizing the slurry; (e) separating the undigested cell residue from the supernatant containing the extracted protein; (f) acidifying the supernatant to precipitate proteins; (g) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (h) neutralizing the mother liquor solution; and (i) drying the mother liquor solution to yield a yeast whippable protein.

Claim 21

The process of claim 16 wherein the precipitated proteins are dried to yield a yeast protein isolate.

Claim 22

The process of claim 16 wherein the precipitated proteins are washed, neutralized, and dried to yield a yeast protein isolate.

Claim 23

The process of claim 16 wherein the food yeast is selected from the group consisting of Candida utilis, Saccharomyces cerevisiae, Saccharomyces fragilis, and Saccharomyces carlsbergensis.

Claim 24

A process for preparing functional food yeast proteins comprising: (a) slurrying whole yeast cells in a dilute alkaline solution having a pH of from about 8.5 to 10.0 at a temperature of from 85° to 95° C. for about from 5 to 30 minutes to extract nucleotidic materials and undesirable flavor and color bodies; (b) separating the yeast cells from the alkaline extract; (c) reslurrying the separated yeast cells in a solution of 0.1 to 0.3N sodium hydroxide at a temperature of from 85° to 100° C. for from 15 to 45 minutes to extract proteins; (d) neutralizing the slurry; (e) separating the undigested cell residue from the supernatant containing the extracted proteins; (f) acidifying the supernatant to a pH of from 3.5 to 4.5 to precipitate proteins; (g) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (h) neutralizing the mother liquor solution to a pH of from 6.7 to 7.2; and (i) spray-drying the mother liquor solution to yield a yeast whippable protein.

Claim 25

A process for preparing functional food yeast proteins comprising: (a) slurrying whole yeast cells in a dilute alkaline solution having a pH of from about 8.5 to 10.0 at a temperature of from 85° to 95° C. for about from 5 to 30 minutes to extract nucleotidic materials and undesirable flavor and color bodies; (b) separating the yeast cells from the alkaline extract; (c) reslurrying the separated yeast cells in a solution of 0.1 to 0.3N sodium hydroxide at a temperature of from 85° to 100° C. for from 15 to 45 minutes to extract proteins; (d) neutralizing the slurry; (e) separating the undigested cell residue from the supernatant containing the extracted proteins; (f) acidifying the supernatant to a pH of from 3.5 to 4.5 to precipitate proteins; (g) separating the precipitated proteins from the mother liquor solution containing the soluble proteins; (h) neutralizing the mother liquor solution to a pH of from 6.7 to 7.2; and (i) spray-drying the mother liquor solution to yield a yeast whippable protein.

Claim 26

The process of claim 20 wherein the precipitated proteins are dissolved by neutralization in a solvent and spray-dried to yield a yeast protein isolate.

Claim 27

The process of claim 20 wherein the food yeast is selected from the group consisting of Candida utilis, Saccharomyces cerevisiae, Saccharomyces fragilis, and Saccharomyces carlsbergensis.

Claim 28

The process of claim 20 wherein the food yeast is of the type Candida utilis.

Claim 29

The process of claim 23 wherein the precipitated proteins are dissolved by neutralization in a solvent and spray-dried to yield a yeast protein isolate.