US4075193A
Expired / Lapsed
US
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Process for producing intravenous immune globulin
Assignee: Oesterreichisches Institut fuer Haemoderivate
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Filing: 1976-11-26
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Granted: 1978-02-21
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Expires: 1996-11-26
Abstract
Abstract A process is provided for producing intravenous immune globulin which comprises adsorbing plasminogen from blood of a selected mammalian species on L-lysine agarose, washing the adsorbate and eluting the purified plasminogen, converting the plasminogen to plasmin, and incubating a mixture of the plasmin and homospecific immune globulin having anticomplementary activity so as to reduce the anticomplementary activity, adsorbing excess plasmin present in the mixture, and recovering the immune globulin from the mixture.
Inventors
- 👤 Cyrill John Campbell
- 👤 Daniel Ting Hsiu Liu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 00:25
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/06; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
91 words
Process for producing immune globulin for intravenous administration which comprises adsorbing plasminogen deriving from blood of a selected mammalian species on an adsorbent substrate of L-lysine agarose, washing the adsorbate to elute impurities, eluting the purified plasminogen from the substrate, converting the eluted plasminogen to plasmin, incubating a mixture of said plasmin and a quantity of homospecific immune globulin having anticomplementary activity under conditions such that the anticomplementary activity is substantially reduced, inactivating plasmin present in said mixture by adsorption on an inactivation adsorbent for plasmin, and recovering the immune globulin.
Claim 2
Dependent Claim
91 words
Process for producing immune globulin for intravenous administration which comprises adsorbing plasminogen deriving from blood of a selected mammalian species on an adsorbent substrate of L-lysine agarose, washing the adsorbate to elute impurities, eluting the purified plasminogen from the substrate, converting the eluted plasminogen to plasmin, incubating a mixture of said plasmin and a quantity of homospecific immune globulin having anticomplementary activity under conditions such that the anticomplementary activity is substantially reduced, inactivating plasmin present in said mixture by adsorption on an inactivation adsorbent for plasmin, and recovering the immune globulin.
Claim 3
Dependent Claim
16 words
Process according to claim 1 wherein the L-lysine agarose substrate is an autoclavable L-lysine cross-linked agarose.
Claim 4
Dependent Claim
22 words
Process according to claim 1 wherein the eluted plasminogen prior to conversion to plasmin is purified by salting out, centrifugation and dialysis.
Claim 5
Dependent Claim
20 words
Process according to claim 1 wherein the plasminogen is converted to plasmin by means of activation with aqueous glycerol solution.
Claim 6
Dependent Claim
24 words
Process according to claim 1 wherein the incubation is carried out using 0.5 to 1.0 unit of plasmin to each milliliter of immune globulin.
Claim 7
Dependent Claim
20 words
Process according to claim 1 wherein the incubation is carried out for a period of from 2 to 9 days.
Claim 8
Dependent Claim
30 words
Process according to claim 1 wherein the incubation is carried out until the anticomplementary activity of the modified immune globulin is at a level of at least about 10 mg./ml.2CH5O.
Claim 9
Dependent Claim
23 words
Process according to claim 1 wherein the incubation is carried out such that fragmentation of the immune globulin is not more than 25%.
Claim 10
Dependent Claim
13 words
Process according to claim 1 wherein the inactivation adsorbent for plasmin is bentonite.
Claim 11
Dependent Claim
16 words
Process according to claim 1 wherein the L-lysine agarose substrate comprises an L-lysine epichlorohydrin cross-linked agarose.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-02-20 | 1981-08-22 | 1982-02-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-02-20 | 1985-08-22 | 1986-02-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-02-20 | 1989-08-22 | 1990-02-21 | $7700.00 | Window Closed / Lapsed |