US4073885A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Anodynin, an endogenous opiate analgesic from human blood, and process for recovering same
Assignee: United States Department of Health and Human Services
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Filing: 1976-09-10
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Granted: 1978-02-14
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Expires: 1996-09-10
Abstract
Abstract A method of recovering a hormone from human blood plasma which has morphine agonist properties which comprises heating said plasma, extracting into butanol to recover anodynin, and purifying said anodynin by centrifuging and ion exchange column separation. Anodynin has a molecular weight of about 600.
Inventors
- 👤 Candace B. Pert
- 👤 Agu Pert
- 👤 John F. Tallman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:11
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/665; C07K14/70; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
71 words
A method of recovering and purifying from human plasma an endogenous composition called anodynin having morphine agonist properties, which comprises acidifying said plasma to a pH of about 3.0 and heating said plasma, adjusting the pH to about 9.0 and recovering a precipitate, extracting said precipitate into butanol to recover anodynin from said butanol extract and reducing to dryness and subsequently purifying said anodynin by centrifuging and ion exchange column separation.
Claim 2
Independent Claim
71 words
A method of recovering and purifying from human plasma an endogenous composition called anodynin having morphine agonist properties, which comprises acidifying said plasma to a pH of about 3.0 and heating said plasma, adjusting the pH to about 9.0 and recovering a precipitate, extracting said precipitate into butanol to recover anodynin from said butanol extract and reducing to dryness and subsequently purifying said anodynin by centrifuging and ion exchange column separation.
Claim 3
Dependent Claim
24 words
The method according to claim 1 wherein the composition having morphine agonist properties is further characterized by an apparent molecular weight of about 600.
Claim 4
Dependent Claim
64 words
The method according to claim 1 wherein the plasma is heated in a boiling water bath and the resulting fluid and precipitate are each extracted into butanol, subsequently the butanol extract is reduced to dryness and redissolved in a buffer at about pH 7.7 at 25° C, and subsequently undissolved material is removed by a low-speed centrifuge and purified in an ion exchange column.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-02-13 | 1981-08-15 | 1982-02-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-02-13 | 1985-08-15 | 1986-02-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-02-13 | 1989-08-15 | 1990-02-14 | $7700.00 | Window Closed / Lapsed |