US4073690A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Citrobacter freundii broth
Assignee: McDonnell Douglas Corp
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Filing: 1976-05-03
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Granted: 1978-02-14
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Expires: 1996-05-03
Abstract
Abstract A broth medium for the detection of Citrobacter freundii in urine. The medium employs palatinose and rhamnose as carbon sources to enhance the growth of the organism. A combination of α-hydroxy-2',4,4'-trichlorodiphenyloxide and Brilliant Green are employed to inhibit the growth of Aeromonas and Klebsiella organisms. Gram positive organisms are inhibited by surfactants, such as Bile Salts mixture. The presence of C. freundii is detected by means of precipitation and a consequent change in the light transmitting properties of the medium.
Inventors
- 👤 Sandra F. Gibson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 14:39
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/24; Y; Y10; Y10S; Y10S435/00; Y10S435/80; Y10S435/8215; Y10S435/822; Y10S435/852; Y10S435/882; Y10S435/883
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
103 words
A broth medium for selective identification of Citrobacter freundii comprising: a. a source of nutrients including at least one sugar that specifically enhances the growth of C. freundii and is fermentable by C. freundii to acidify the said broth, said sugars being selected from palatinose, rhamnose, and combinations thereof, b. a gram-positive inhibitor, and c. an inhibitor to inhibit the growth of organisms that give false positive results in tests for C. freundii, d. said broth medium being responsive to a pH change toward and induced by said sugar fermentation by C. freundii to indicate the presence of C. freundii in said broth.
Claim 2
Dependent Claim
103 words
A broth medium for selective identification of Citrobacter freundii comprising: a. a source of nutrients including at least one sugar that specifically enhances the growth of C. freundii and is fermentable by C. freundii to acidify the said broth, said sugars being selected from palatinose, rhamnose, and combinations thereof, b. a gram-positive inhibitor, and c. an inhibitor to inhibit the growth of organisms that give false positive results in tests for C. freundii, d. said broth medium being responsive to a pH change toward and induced by said sugar fermentation by C. freundii to indicate the presence of C. freundii in said broth.
Claim 3
Dependent Claim
15 words
The medium of claim 1 wherein about 9 to about 11 gm/l palatinose is used.
Claim 4
Dependent Claim
18 words
The medium of claim 1 including a source of amino acids in the nutrient portion of the medium.
Claim 5
Dependent Claim
12 words
The medium of claim 3 including Trypticase as the amino acid source.
Claim 6
Dependent Claim
12 words
The medium of claim 1 wherein the false positive inhibitor is α-hydroxy-2',4,4'-trichloro-diphenyloxide.
Claim 7
Dependent Claim
28 words
The medium of claim 5 wherein the equivalent of about 14.5 to about 15.5 gm/l of medium of a 1% solution of α-hydroxy-2',4,4'-trichloro-diphenyloxide is included as an inhibitor.
Claim 8
Dependent Claim
14 words
The medium of claim 1 wherein the gram positive inhibitor is Bile Salts Mixture.
Claim 9
Dependent Claim
19 words
The medium of claim 1 containing C. freundii and a precipitate produced by the presence of said C. freundii.
Claim 10
Dependent Claim
84 words
The medium of claim 1 comprising per liter of medium: A. about 4.5 to about 5.5 gms amino acid source, B. about 9 to about 11 gms surfactants, C. about 9 to about 11 gms palatinose, D. about 9 to about 11 gms rhamnose, E. about 52 to about 55 mg Brilliant Green, and F. the equivalent of about 14.5 to about 15.5 ml of a 1% solution of α-hydroxy-2',4,4'-trichloro-diphenyloxide, and G. distilled water, H. the medium being at a pH of about 7.5.
Claim 11
Dependent Claim
55 words
The medium of claim 1 comprising per liter of medium: A. about 5 gm Gelysate, B. about 10 gm Bile Salts Mixture, C. about 10 gm palatinose, D. about 10 gm rhamnose, E. about 53 mgm Brilliant Green, F. the equivalent of about 15 ml of a 1% solution of α-hydroxy-2',4,4'-trichloro-diphenyloxide, and G. distilled water.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-02-13 | 1981-08-15 | 1982-02-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-02-13 | 1985-08-15 | 1986-02-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-02-13 | 1989-08-15 | 1990-02-14 | $7700.00 | Window Closed / Lapsed |