Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
30 words
A method for the preparation of a neuraminidase which comprises cultivating the microorganism Arthrobacter sialophilum ATCC 31253 or variant or derivative thereof in the presence of a neuraminidase-inducing substance therefor.
Claim 2
Independent Claim
30 words
A method for the preparation of a neuraminidase which comprises cultivating the microorganism Arthrobacter sialophilum ATCC 31253 or variant or derivative thereof in the presence of a neuraminidase-inducing substance therefor.
Claim 3
Dependent Claim
19 words
A method in accordance with claim 1 wherein the neuraminidase-inducing substance comprises a glycoprotein or low molecular weight glycopeptides.
Claim 4
Dependent Claim
27 words
A method in accordance with claim 1 wherein the neuraminidase-inducing substance comprises bird's nest, an edible regurgitated gelatinous material produced by the swiftlets of the genus Collocalia.
Claim 5
Dependent Claim
15 words
A method in accordance with claim 1 wherein said neuraminidase-inducing substance is acid-treated bird's nest.
Claim 6
Dependent Claim
19 words
A method in accordance with claim 1 wherein said neuraminidase-inducing substance comprises a hot water extract of bird's nest.
Claim 7
Independent Claim
41 words
A method for the preparation of a neuraminidase which comprises cultivating the microorganism Arthrobacter sialophilum ATCC 31253 or variant or derivative thereof, harvesting the resulting produced microorganisms and inducing neuraminidase therefrom by contacting the harvested microorganisms with a neuraminidase-inducing substance therefor.
Claim 8
Independent Claim
41 words
A method for the preparation of a neuraminidase which comprises cultivating the microorganism Arthrobacter sialophilum ATCC 31253 or variant or derivative thereof, harvesting the resulting produced microorganisms and inducing neuraminidase therefrom by contacting the harvested microorganisms with a neuraminidase-inducing substance therefor.
Claim 9
Dependent Claim
16 words
A method in accordance with claim 6 wherein said neuraminidase-inducing substance comprises a glycoprotein or glycopeptides.
Claim 10
Dependent Claim
14 words
A method in accordance with claim 6 wherein said neuraminidase-inducing substance comprises bird's nest.
Claim 11
Dependent Claim
15 words
A method in accordance with claim 6 wherein said neuraminidase-inducing substance comprises acid-treated bird's nest.
Claim 12
Dependent Claim
20 words
A method in accordance with claim 6 wherein said neuraminidase-inducing substance comprises an acid-treated hot water extract of bird's nest.
Claim 13
Dependent Claim
29 words
A method in accordance with claim 6 wherein the harvested microorganisms are contacted with an aqueous salt solution prior to inducing neuraminidase therefrom by contact with a neuraminidase-inducing substance.
Claim 14
Dependent Claim
15 words
A method in accordance with claim 11 wherein said aqueous salt solution comprises sodium chloride.
Claim 15
Dependent Claim
79 words
Extracellular neuraminidase derived from the microorganism Arthrobacter sialophilum ATCC 31253 or a neuraminidase-producing mutant or variant or derivative thereof, said neuraminidase having a molecular weight of about 87,000 daltons as measured by gel filtration chromatography, a pH optimum of 5-6, an apparent K m of about 2.08 mg/ml for Collocalia mucoid and 3.3 × 10 -3 for N-acetylneuraminlactose, being insensitive to both Ca ++ ions and EDTA and capable of hydrolyzing (α, 2-3), (α, 2-6) or (α, 2-8) linkages.
Claim 16
Dependent Claim
79 words
Extracellular neuraminidase derived from the microorganism Arthrobacter sialophilum ATCC 31253 or a neuraminidase-producing mutant or variant or derivative thereof, said neuraminidase having a molecular weight of about 87,000 daltons as measured by gel filtration chromatography, a pH optimum of 5-6, an apparent K m of about 2.08 mg/ml for Collocalia mucoid and 3.3 × 10 -3 for N-acetylneuraminlactose, being insensitive to both Ca ++ ions and EDTA and capable of hydrolyzing (α, 2-3), (α, 2-6) or (α, 2-8) linkages.