US4069314A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Glycopeptide extract
Assignee: Individual
•
Filing: 1975-06-19
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Granted: 1978-01-17
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Expires: 1995-06-19
Abstract
Abstract Immunostimulating glycopeptides obtainable from the cell walls of immunostimulating bacteria belonging to the Mycobacteriaceae or Actinomycetaceae Family and methods of isolating the glycopeptides.
Inventors
- 👤 Christopher Adlam
- 👤 David Eric Reid
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 22:12
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/34; C12; C12P; C12P21/00; C12P21/005
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
149 words
An immunostimulating glycopeptide suitable for use in conferring on mammals or birds a resistance to Sarcoma 180T/G tumour and pathogenic microoganisms which glycopeptide is obtainable from the cell wall of an immunostimulating bacterium belonging to the Actinomycetaceae family of Prevot's classification, which glycopeptide is substantially insoluble in water, physiological saline, methanol, ethanol, phenol, chloroform, dioxan, pyridine, dimethylformamide, diethylene glycol, and hexane; has an absorption in the Infra-red spectrum at 1550 and 1660 cm -1 consistent with the presence of peptide; has an absorption in the Infra-red spectrum between 950 and 1100 cm -1 with a maximum absorption at 1050 to 1070 cm -1 consistent with the presence of carbohydrate; has little or no lipid absorption in the Infra-red spectrum at 2850 to 2950 cm -1 ; and which glycopeptide, after intravenous administration, increases the liver and spleen weight of mice, and increases the life expectancy of mice with tumours,
Claim 2
Dependent Claim
149 words
An immunostimulating glycopeptide suitable for use in conferring on mammals or birds a resistance to Sarcoma 180T/G tumour and pathogenic microoganisms which glycopeptide is obtainable from the cell wall of an immunostimulating bacterium belonging to the Actinomycetaceae family of Prevot's classification, which glycopeptide is substantially insoluble in water, physiological saline, methanol, ethanol, phenol, chloroform, dioxan, pyridine, dimethylformamide, diethylene glycol, and hexane; has an absorption in the Infra-red spectrum at 1550 and 1660 cm -1 consistent with the presence of peptide; has an absorption in the Infra-red spectrum between 950 and 1100 cm -1 with a maximum absorption at 1050 to 1070 cm -1 consistent with the presence of carbohydrate; has little or no lipid absorption in the Infra-red spectrum at 2850 to 2950 cm -1 ; and which glycopeptide, after intravenous administration, increases the liver and spleen weight of mice, and increases the life expectancy of mice with tumours,
Claim 3
Dependent Claim
18 words
A glycopeptide as claimed in claim 1 wherein the immunostimulating bacterium is a member of the Corynebacterium genus.
Claim 4
Dependent Claim
19 words
A glycopeptide as claimed in claim 2 wherein the immunostimulating bacterium is a member of the Corynebacterium parvum species.
Claim 5
Dependent Claim
60 words
A process for obtaining a glycopeptide as defined in claim 1 comprising the steps of culturing the immunostimulating Actinomycetaceae bacterial cells; recovering cultured bacterial cells from the culture; and subjecting the cultured bacterial cells to biphasic solvent extraction using one phase comprising water and a second phase comprising an aryl alcohol to as to yield the glycopeptide as insoluable residue.
Claim 6
Dependent Claim
21 words
A process as claimed in claim 4 wherein the cultured bacterial cells are subjected to lysis prior to biphasic solvent extraction.
Claim 7
Dependent Claim
70 words
A process as claimed in claim 4 wherein the biphasic solvent extraction comprises the steps of contacting the bacterial cells and/or a lysate thereof with the water and the aryl alcohol followed by centrifugation of the resulting mixture, removal of the aqueous phase and removal of the alcoholic phase to yield a sediment containing an upper layer comprising a white finely divided suspension which is collected to yield the glycopeptide.
Claim 8
Dependent Claim
14 words
A process as claimed in claim 6 wherein the aryl alcohol is unsubstituted phenol.
Claim 9
Dependent Claim
28 words
A glycopeptide as claimed in claim 1 wherein the immunostimulating bacterium belongs to the strain of the Corynebacterium parvum species having the Wellcome Culture Collection No. CN 6134.
Claim 10
Dependent Claim
17 words
A process as claimed in claim 4 wherein the immunostimulating bacterial cells are of the Corynebacterium genus.
Claim 11
Dependent Claim
18 words
A process as claimed in claim 9 wherein the immunostimulating bacterial cells are of the Corynebacterium parvum species.
Claim 12
Dependent Claim
29 words
A process as claimed in claim 4 wherein the immunostimulating bacterial cells are of the strain of the Corynebacterium parvum species having the Wellcome Culture Collection No. CN 6134.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-16 | 1981-07-18 | 1982-01-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-16 | 1985-07-18 | 1986-01-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-16 | 1989-07-18 | 1990-01-17 | $7700.00 | Window Closed / Lapsed |