US4069106A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of enzymes on keratin
Assignee: United States Department of Agriculture
•
Filing: 1977-01-13
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Granted: 1978-01-17
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Expires: 1997-01-13
Abstract
Abstract Insolubilized but active enzymes are prepared by mixing an aqueous solution of the enzyme with reduced keratin-containing material. The invention is particularly useful for insolubilizing sulfhydryl-containing enzymes such as urease.
Inventors
- 👤 William L. Stanley
- 👤 Glenn G. Watters
- 👤 Bock G. Chan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 17:42
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
75 words
A process for preparing an insoluble but active enzyme, which comprises -- a. reacting keratin-containing material with a sulfur-containing, reductive, disulphide-splitting agent to reduce keratin and produce a reduced keratin-containing material, b. dissolving a soluble sulfhydryl group containing enzyme in water, and c. mixing said reduced keratin-containing material with the solution of the enzyme in water so that the enzyme reacts directly with the reduced keratin and becomes chemically bonded to said reduced keratin-containing material.
Claim 2
Dependent Claim
75 words
A process for preparing an insoluble but active enzyme, which comprises -- a. reacting keratin-containing material with a sulfur-containing, reductive, disulphide-splitting agent to reduce keratin and produce a reduced keratin-containing material, b. dissolving a soluble sulfhydryl group containing enzyme in water, and c. mixing said reduced keratin-containing material with the solution of the enzyme in water so that the enzyme reacts directly with the reduced keratin and becomes chemically bonded to said reduced keratin-containing material.
References Cited (Backward Citations)
US2474339A
Cited
US3977941A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-16 | 1981-07-18 | 1982-01-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-16 | 1985-07-18 | 1986-01-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-16 | 1989-07-18 | 1990-01-17 | $7700.00 | Window Closed / Lapsed |