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US4066511A Expired / Lapsed US ⏱️ 0.0 years remaining

Analytic device and method

Assignee: Individual
•
Filing: 1975-08-11
•
Granted: 1978-01-03
•
Expires: 1995-08-11
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Abstract

Abstract The present invention provides an apparatus useful for the detection of an unknown microorganism such as a bacterium in a liquid nutritive medium comprising a tube adapted to receive a portion of a nutritive liquid medium containing an unknown bacterium and means for sealing off said tube and a test strip comprising at least one liquid reactive surface capable of being immersed in said liquid medium, said surface containing nutritive elements complementary to the nutritive elements in the liquid medium and a reagent which reacts with said bacterium, said reagent being incapable of inhibiting growth of said specimen and being non-toxic to said specimen, and at least one vapor-reactive surface incapable of being immersed in the liquid medium when said reactive surface is immersed in the liquid medium, said surface containing reagents which are toxic to said bacterium and react on contact with vapors emitted by the liquid medium due to the presence of the bacterium contained thereon. The invention also provides a novel method for detecting a bacterium, a test strip and a flexible cover for glass containers.

Inventors

  • 👤 Paul A. F. Montagnon

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-30 01:19
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/08; C12M23/38; C12M41/00; C12M41/30; C12M41/36; Y; Y10; Y10S; Y10S435/00; Y10S435/81

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 195 words
An analytical testing kit adapted to detect the presence of at least one of a plurality of different predetermined microorganisms in a test specimen comprising: a. a tubular receptacle having a portion therein containing a liquid medium having both nutrients for enabling the growth of microorganisms of the specimen when disposed therein and a first predetermined reagent which can react with a predetermined microorganism of the plurality to give an indication, the tubular receptacle additionally having means for sealing the liquid medium within the tube; and b. an elongated test strip adapted to be inserted into the tubular receptacle when the sealing means is removed, the test strip having 1. at least one liquid-reactive region adjacent one end portion of the strip adapted to be immersed in the liquid medium, the liquid-reactive region containing other nutrients complementary to the nutrients of the liquid medium and a second predetermined reagent which can react to give an indication when another predetermined microorganism of the plurality is cultured in the liquid medium, the second predetermined reagent being incapable of inhibiting the growth of the plurality of different microorganism and being non-toxic to the plurality of microorganisms; and 2.
Claim 2
Dependent Claim 195 words
An analytical testing kit adapted to detect the presence of at least one of a plurality of different predetermined microorganisms in a test specimen comprising: a. a tubular receptacle having a portion therein containing a liquid medium having both nutrients for enabling the growth of microorganisms of the specimen when disposed therein and a first predetermined reagent which can react with a predetermined microorganism of the plurality to give an indication, the tubular receptacle additionally having means for sealing the liquid medium within the tube; and b. an elongated test strip adapted to be inserted into the tubular receptacle when the sealing means is removed, the test strip having 1. at least one liquid-reactive region adjacent one end portion of the strip adapted to be immersed in the liquid medium, the liquid-reactive region containing other nutrients complementary to the nutrients of the liquid medium and a second predetermined reagent which can react to give an indication when another predetermined microorganism of the plurality is cultured in the liquid medium, the second predetermined reagent being incapable of inhibiting the growth of the plurality of different microorganism and being non-toxic to the plurality of microorganisms; and 2.
Claim 3
Dependent Claim 63 words
at least one vapor-reactive region, the vapor-reactive region being adapted to be disposed out of the liquid medium when the liquid-reactive region is immersed in the liquid medium, said vapor-reactive region containing a third predetermined reagent which can react to give an indication when contacted with a predetermined vapor emitted from the liquid medium in response to the presence of a predetermined microorganism
Claim 4
Dependent Claim 63 words
at least one vapor-reactive region, the vapor-reactive region being adapted to be disposed out of the liquid medium when the liquid-reactive region is immersed in the liquid medium, said vapor-reactive region containing a third predetermined reagent which can react to give an indication when contacted with a predetermined vapor emitted from the liquid medium in response to the presence of a predetermined microorganism
Claim 5
Dependent Claim 256 words
of the plurality of microorganisms. 2. An analytical testing strip for detecting the presence of at least one of a plurality of different microorganisms in a test specimen, the testing strip being elongated and adapted to be inserted into a tubular receptacle having a portion therein containing a liquid medium having both nutrients for enabling the growth of microorganisms of the specimen when disposed therein and a first predetermined reagent which can react with a predetermined microorganism of the plurality to give an indication, the tubular receptacle additionally having means for sealing the liquid medium within the tube, the testing strip comprising: a. at least one liquid-reactive region adjacent one end portion of the strip adapted to be immersed in the liquid medium, the liquid-reactive region containing other nutrients complementary to the nutrients of the liquid medium and a second predetermined reagent which can react to give an indication when another predetermined microorganism of the plurality is cultured in the liquid medium, the second predetermined reagent being incapable of inhibiting the growth of the plurality of different microorganism and being non-toxic to the plurality of microorganisms; and b. at least one vapor-reactive region, the vapor-reactive region being adapted to be disposed out of the liquid medium when the liquid-reactive region is immersed in the liquid medium, said vapor-reactive region containing a third predetermined reagent which can react to give an indication when contacted with a predetermined vapor emitted from the liquid medium in response to the presence of a predetermined microorganism of the plurality of microorganisms.
Claim 6
Dependent Claim 256 words
of the plurality of microorganisms. 2. An analytical testing strip for detecting the presence of at least one of a plurality of different microorganisms in a test specimen, the testing strip being elongated and adapted to be inserted into a tubular receptacle having a portion therein containing a liquid medium having both nutrients for enabling the growth of microorganisms of the specimen when disposed therein and a first predetermined reagent which can react with a predetermined microorganism of the plurality to give an indication, the tubular receptacle additionally having means for sealing the liquid medium within the tube, the testing strip comprising: a. at least one liquid-reactive region adjacent one end portion of the strip adapted to be immersed in the liquid medium, the liquid-reactive region containing other nutrients complementary to the nutrients of the liquid medium and a second predetermined reagent which can react to give an indication when another predetermined microorganism of the plurality is cultured in the liquid medium, the second predetermined reagent being incapable of inhibiting the growth of the plurality of different microorganism and being non-toxic to the plurality of microorganisms; and b. at least one vapor-reactive region, the vapor-reactive region being adapted to be disposed out of the liquid medium when the liquid-reactive region is immersed in the liquid medium, said vapor-reactive region containing a third predetermined reagent which can react to give an indication when contacted with a predetermined vapor emitted from the liquid medium in response to the presence of a predetermined microorganism of the plurality of microorganisms.
Claim 7
Dependent Claim 436 words
A process for detecting the presence of at least one of a plurality of different predetermined microorganisms in a test specimen by use of a tubular receptacle having a portion therein containing a liquid medium having both nutrients for enabling the growth of microorganisms of the specimen when disposed therein and a first predetermined reagent which can react with a predetermined microorganism of the plurality to give an indication, the tubular receptacle additionally having means for sealing the liquid medium within the tube, and an elongated test strip adapted to be inserted into the tubular receptacle when the sealing means is removed, the test strip having at least one liquid-reactive region adjacent one end portion of the strip adapted to be immersed in the liquid medium, the liquid-reactive region containing other nutrients complementary to the nutrients of the liquid medium and a second predetermined reagent which can react to give an indication when another predetermined microorganism of the plurality is cultured in the liquid medium, the second predetermined reagent being incapable of inhibiting the growth of the plurality of different microorganism and being non-toxic to the plurality of microorganisms, and at least one vapor-reactive region, the vapor-reactive region being adapted to be disposed out of the liquid medium when the liquid-reactive region is immersed in the liquid medium, said vapor-reactive region containing a third predetermined reagent which can react to give an indication when contacted with a predetermined vapor emitted from the liquid medium in response to the presence of a predetermined microorganism of the plurality of microorganisms, the process comprising the steps of: a. placing the test specimen into the liquid medium in the tubular receptacle, the nutrient in the liquid medium enabling the test specimen to be cultured, b. placing the elongated test strip into the tubular receptacle when the sealing means is removed with the liquid-reactive region of the test strip immersed in the liquid medium and the vapor-reactive region disposed out of the liquid medium, c. permitting the test specimen to be cultured, to react with any one of the first and second reagents, and any vapor from the liquid medium to react with the third reagent, d. sensing the liquid medium for a possible indication by the first reagent, e. further sensing the liquid-reactive portion of the test strip for possible indication by the second reagent, f. additionally sensing the vapor-reactive region of the test strip for possible indication by the third reagent, and g. compiling the results of the steps of (c), (d), and (e) for determining the presence of at least one of a plurality of different predetermined microorganisms.
Claim 8
Dependent Claim 436 words
A process for detecting the presence of at least one of a plurality of different predetermined microorganisms in a test specimen by use of a tubular receptacle having a portion therein containing a liquid medium having both nutrients for enabling the growth of microorganisms of the specimen when disposed therein and a first predetermined reagent which can react with a predetermined microorganism of the plurality to give an indication, the tubular receptacle additionally having means for sealing the liquid medium within the tube, and an elongated test strip adapted to be inserted into the tubular receptacle when the sealing means is removed, the test strip having at least one liquid-reactive region adjacent one end portion of the strip adapted to be immersed in the liquid medium, the liquid-reactive region containing other nutrients complementary to the nutrients of the liquid medium and a second predetermined reagent which can react to give an indication when another predetermined microorganism of the plurality is cultured in the liquid medium, the second predetermined reagent being incapable of inhibiting the growth of the plurality of different microorganism and being non-toxic to the plurality of microorganisms, and at least one vapor-reactive region, the vapor-reactive region being adapted to be disposed out of the liquid medium when the liquid-reactive region is immersed in the liquid medium, said vapor-reactive region containing a third predetermined reagent which can react to give an indication when contacted with a predetermined vapor emitted from the liquid medium in response to the presence of a predetermined microorganism of the plurality of microorganisms, the process comprising the steps of: a. placing the test specimen into the liquid medium in the tubular receptacle, the nutrient in the liquid medium enabling the test specimen to be cultured, b. placing the elongated test strip into the tubular receptacle when the sealing means is removed with the liquid-reactive region of the test strip immersed in the liquid medium and the vapor-reactive region disposed out of the liquid medium, c. permitting the test specimen to be cultured, to react with any one of the first and second reagents, and any vapor from the liquid medium to react with the third reagent, d. sensing the liquid medium for a possible indication by the first reagent, e. further sensing the liquid-reactive portion of the test strip for possible indication by the second reagent, f. additionally sensing the vapor-reactive region of the test strip for possible indication by the third reagent, and g. compiling the results of the steps of (c), (d), and (e) for determining the presence of at least one of a plurality of different predetermined microorganisms.

References Cited (Backward Citations)

US3249513A Cited
US3455656A Cited
US3616258A Cited
US3802842A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1981-01-02 1981-07-04 1982-01-03 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1985-01-02 1985-07-04 1986-01-03 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1989-01-02 1989-07-04 1990-01-03 $7700.00 Window Closed / Lapsed

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