US4066505A
Expired / Lapsed
US
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Process for extracting a polypeptide from an aqueous solution
Assignee: Battelle Memorial Institute Inc
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Filing: 1976-03-16
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Granted: 1978-01-03
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Expires: 1996-03-16
Abstract
Abstract A polypeptide is extracted from an aqueous solution by a process in which a water-soluble macromolecular complex (I) of macromolecules covalently boned to molecules of at least one compound capable of fixing the polypeptide in a selective, reversible and non-destructive manner, is selectively reacted with the polypeptide in the solution to fix the polypeptide by forming in solution a complex (II); and the complex (II) is separated from the solution and dissociated into polypeptide molecules and complex (I) molecules, and the polypeptide is isolated.
Inventors
- 👤 Michel Schneider
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 09:56
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0071; C07; C07K; C07K1/00; C07K1/14; C07K1/36; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6427; C12N9/78; C12N9/80; C12N9/82; C12Y; C12Y114/00; C12Y114/18; C12Y114/18001; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
84 words
A process for extracting a polypeptide from an aqueous solution, in which a dissolved water-soluble macromolecular complex (I) of macromolecules covalently bonded to molecules of at least one compound capable of fixing the polypeptide in a selective, reversible and non-destructive manner, is selectively reacted with the polypeptide in the solution to fix the polypeptide by forming, in solution, a complex (II); and the complex (II) is separated from the solution, then dissociated into polypeptide molecules and complex (I) molecules, and the polypeptide is isolated.
Claim 2
Dependent Claim
84 words
A process for extracting a polypeptide from an aqueous solution, in which a dissolved water-soluble macromolecular complex (I) of macromolecules covalently bonded to molecules of at least one compound capable of fixing the polypeptide in a selective, reversible and non-destructive manner, is selectively reacted with the polypeptide in the solution to fix the polypeptide by forming, in solution, a complex (II); and the complex (II) is separated from the solution, then dissociated into polypeptide molecules and complex (I) molecules, and the polypeptide is isolated.
Claim 3
Dependent Claim
23 words
A process according to claim 1 in which the complex (II) is separated as a precipitate by adjusting the pH of the solution.
Claim 4
Dependent Claim
35 words
A process according to claim 1 in which an enzyme is extracted using a complex (I) containing molecules of substances selected from the group consisting of reversible inhibitors, substrates and substrate analogues of the enzyme.
Claim 5
Dependent Claim
39 words
A process according to claim 1 in which the complex (I) comprises the said molecules, bonded to a macromolecular substance selected from the group consisting of a polyacrylamide, carboxymethyl cellulose, a polyethylene glycol, a polyamine, polyvinyl alcohol and dextran.
Claim 6
Dependent Claim
36 words
A process according to claim 1 in which the said molecules are molecules selected from the group comprising p-aminobenzoic acid, o-aminobenzoic acid, trypsin, N-ω-aminohexyl-L-aspartic acid, trypsin-inhibiting soya peptide, p-aminophenyl-thiogalactoside, poly-L-lysine, nicotinamide adenine dinucleotide (NAD) and D-asparagine.
Claim 7
Dependent Claim
29 words
A process according to claim 1 in which the complex (I) is formed by grafting molecules of the said compound capable of fixing the polypeptide, onto a macromolecular substance.
Claim 8
Dependent Claim
40 words
A process according to claim 1 in which the complex (I) is formed by polymerizing a monomer, to each molecule of which at least one molecule of the said compound capable of fixing the polypeptide is fixed by covalent bonding.
Claim 9
Dependent Claim
20 words
A process according to claim 4 in which the complex (I) comprises molecules of p-aminobenzoic acid bonded to a polyacrylamide.
References Cited (Backward Citations)
US3834990A
Cited
US3983001A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-02 | 1981-07-04 | 1982-01-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-02 | 1985-07-04 | 1986-01-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-02 | 1989-07-04 | 1990-01-03 | $7700.00 | Window Closed / Lapsed |