Fixing and retrieving recorded information produced by means of photosensitive material of biological origin

Claim 1

A method of recording and reproducing information comprising the steps of: providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier; information-wise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information; fixing said layer by denaturation of the protein of said photoactive product; and retrieving the recorded information from said layer by detecting a pattern of differences in intensity of light absorption of the layer; said photoactive product being formed by the steps of: extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5° C; adding to said binary complex at a temperature of at most 5° C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate; cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material; lyophilizing said product at a temperature of at most 5° C so that the dried final product remains photoactive at room temperature; and providing said product in layer form suitable for recording.

Claim 2

A method of recording and reproducing information comprising the steps of: providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier; information-wise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information; fixing said layer by denaturation of the protein of said photoactive product; and retrieving the recorded information from said layer by detecting a pattern of differences in intensity of light absorption of the layer; said photoactive product being formed by the steps of: extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5° C; adding to said binary complex at a temperature of at most 5° C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate; cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material; lyophilizing said product at a temperature of at most 5° C so that the dried final product remains photoactive at room temperature; and providing said product in layer form suitable for recording.

Claim 5

A method of recording and reproducing information comprising the steps of: providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier; information-wise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information; fixing said layer by denaturation of the protein of said photoactive product; and retrieving the recorded information from said layer by subjecting the layer to light and measuring fluorescence emission from said layer; said photoactive product being formed by the steps of: extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5° C; adding to said binary complex at a temperature of at most 5° C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate; cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material; lyophilizing said product at a temperature of at most 5° C so that the dried final product remains photoactive at room temperature; and providing said product in layer form suitable for recording.

Claim 6

A method of recording and reproducing information comprising the steps of: providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier; information-wise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information; fixing said layer by denaturation of the protein of said photoactive product; and retrieving the recorded information from said layer by subjecting the layer to light and measuring fluorescence emission from said layer; said photoactive product being formed by the steps of: extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5° C; adding to said binary complex at a temperature of at most 5° C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate; cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material; lyophilizing said product at a temperature of at most 5° C so that the dried final product remains photoactive at room temperature; and providing said product in layer form suitable for recording.

Claim 9

A method of recording and reproducing information comprising the steps of: providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier; informationwise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information; fixing said layer by subjecting said layer subsequent to recordal of information thereon to a temperature below -100° C; and retrieving the recorded information from said layer by detecting a pattern of differences of intensity of light absorption of the layers; said photoactive product being formed by the steps of: extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5° C; adding to said binary complex at a temperature of at most 5° C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate; cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material; lyophilizing said product at a temperature of at most 5° C so that the dried final product remains photoactive at room temperature; and providing said product in layer form suitable for recording.

Claim 10

A method of recording and reproducing information comprising the steps of: providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier; informationwise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information; fixing said layer by subjecting said layer subsequent to recordal of information thereon to a temperature below -100° C; and retrieving the recorded information from said layer by detecting a pattern of differences of intensity of light absorption of the layers; said photoactive product being formed by the steps of: extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5° C; adding to said binary complex at a temperature of at most 5° C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate; cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material; lyophilizing said product at a temperature of at most 5° C so that the dried final product remains photoactive at room temperature; and providing said product in layer form suitable for recording.

Claim 11

A method of recording and reproducing information comprising the steps of: providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier; informationwise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information; fixing said layer by subjecting said layer subsequent to recordal of information thereon to a temperature below -100° C; and retrieving the recorded information from said layer by subjecting the layer to light and measuring fluorescence emission from said layer; said photoactive product being formed by the steps of: extraction of an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5° C; adding to said binary complex at a temperature of at most 5° C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate; cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material; lyophilizing said product at a temperature of at most 5° C so that the dried final product remains photoactive at room tepmerature; and providing said product in layer form suitable for recording.

Claim 12

A method of recording and reproducing information comprising the steps of: providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier; informationwise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information; fixing said layer by subjecting said layer subsequent to recordal of information thereon to a temperature below -100° C; and retrieving the recorded information from said layer by subjecting the layer to light and measuring fluorescence emission from said layer; said photoactive product being formed by the steps of: extraction of an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5° C; adding to said binary complex at a temperature of at most 5° C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate; cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material; lyophilizing said product at a temperature of at most 5° C so that the dried final product remains photoactive at room tepmerature; and providing said product in layer form suitable for recording.