US4061828A
Expired / Lapsed
US
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Modified, grafted mineral carriers
Assignee: Rhone Poulenc Industries SA
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Filing: 1976-03-26
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Granted: 1977-12-06
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Expires: 1996-03-26
Abstract
Abstract The invention concerns mineral carriers carrying modified silicon grafts. They comprise mineral carriers grafted by silicon derivatives with aromatic amino substituents, modified by substituted or unsubstituted adenine radicals. The carriers are used in chromatography and more particularly in affinity chromatography for the purification of enzymes.
Inventors
- 👤 Honore Mazarguil
- 👤 Francois Meiller
- 👤 Pierre Monsan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 08:38
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/02; B01J20/10; B01J20/103; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28014; B01J20/28016; B01J20/28019; B01J20/28054; B01J20/28057; B01J20/28069; B01J20/28078; B01J20/281; B01J20/286; B01J20/30; B01J20/3071; B01J20/3078; B01J20/3085; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3214; B01J20/3217; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J20/3259; B01J20/3261; B01J20/3263; B01J20/3268; B01J20/3272; B01J20/3274; B01J20/3276; B01J20/3278; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2998; Y10T428/31504; Y10T428/31551; Y10T428/31609; Y10T428/31612; Y10T428/31652; Y10T428/31663
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
98 words
A method of preparing carriers comprising grafting onto a mineral carrier, a silicon derivative, diazotizing the grafted mineral carrier, and then fixing adenine or its derivative onto the diazotized grafted mineral carrier in which the silicon derivative to be grafted is of the general formula ##STR4## wherein A, B and C, which may be similar or different, are selected from the group consisting of methoxy, ethoxy, methyl or ethyl, provided that at least one of them is reactive with an Oh group in the mineral carrier, n being a whole number with a value from 2 to 4.
Claim 2
Independent Claim
98 words
A method of preparing carriers comprising grafting onto a mineral carrier, a silicon derivative, diazotizing the grafted mineral carrier, and then fixing adenine or its derivative onto the diazotized grafted mineral carrier in which the silicon derivative to be grafted is of the general formula ##STR4## wherein A, B and C, which may be similar or different, are selected from the group consisting of methoxy, ethoxy, methyl or ethyl, provided that at least one of them is reactive with an Oh group in the mineral carrier, n being a whole number with a value from 2 to 4.
Claim 3
Dependent Claim
42 words
A method of preparing carriersof claim 1, comprising grafting onto a mineral carrier a silicon derivative having an aromatic amino radical as a substituent, diazotizing the grafting mineral carrier, and then fixing adenine or its derivative onto the diazotized grafted mineral carrier.
Claim 4
Dependent Claim
54 words
A method according to claim 1, in which the mineral carrier has hydroxyl groups and has a grain size of 40 μ m to 2.5 nm, a specific surface area of 1 to 600 m 2 /g, a pore diameter of 40 to 10,000 A and a pore volume of 0.5 to 1.8 ml/g.
Claim 5
Dependent Claim
22 words
A method according to claim 1 in which the silicon derivative to be grafted is a polymer or copolymer of the silane.
Claim 6
Dependent Claim
39 words
A method according to claim 1, in which the adenine derivative is selected from the group consisting of adenosine, adenosine monophosphate, adenosine diphosphate, adenosine triphosphate, adenylyl-imidodiphosphate, coenzyme A, flavine adenine dinucleotide, nicotinamide adenine dinucleotide, deoxyribonucleic acid and ribonucleic acid.
Claim 7
Dependent Claim
45 words
A method according to claim 1, in which the adenine or its derivatives are fixed on the diazotized grafted carrier in a medium adapted to dissolve the adenine or its derivatives, at an alkaline pH and at a temperature which does not exceed room temperature.
Claim 8
Dependent Claim
82 words
Mineral carriers grafted with silicon derivatives having aromatic amino substituents in which the silicon derivative has the general formula ##STR5## wherein A, B and C, which may be similar or different, are selected from the group consisting of methoxy, ethoxy, methyl or ethyl, provided that at least one of them is reactive with an OH group in the mineral carrier, n being a whole number with a value from 2 to 4, and said grafts modified by substituted or unsubstituted adenine radicals.
Claim 9
Dependent Claim
82 words
Mineral carriers grafted with silicon derivatives having aromatic amino substituents in which the silicon derivative has the general formula ##STR5## wherein A, B and C, which may be similar or different, are selected from the group consisting of methoxy, ethoxy, methyl or ethyl, provided that at least one of them is reactive with an OH group in the mineral carrier, n being a whole number with a value from 2 to 4, and said grafts modified by substituted or unsubstituted adenine radicals.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-05 | 1981-06-06 | 1981-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-05 | 1985-06-06 | 1985-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-05 | 1989-06-06 | 1989-12-06 | $7700.00 | Window Closed / Lapsed |