US4060933A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Tissue culture technique utilizing a specific light source
Assignee: Verizon Laboratories Inc
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Filing: 1976-12-20
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Granted: 1977-12-06
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Expires: 1996-12-20
Abstract
Abstract One aspect of the invention comprises a method for stimulating organogenesis of explants in a tissue culture comprising the step of illuminating the explant culture during at least a portion of its differentiation stage with light having a predominate spectral emission of approximately 660nm.
Inventors
- 👤 Prakash G. Kadkade
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 02:45
CPC Classifications
A; A01; A01G; A01G7/00; A01G7/04; A01G7/045; A01H; A01H4/00; Y; Y02; Y02P; Y02P60/00; Y02P60/14; Y10; Y10S; Y10S47/00; Y10S47/06
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
38 words
A method for stimulating organogenesis of explants in a tissue culture medium comprising the step of: illuminating the explant during at least a portion of its differentiation stage with light having a predominate spectral emission at approximately 660nm.
Claim 2
Independent Claim
38 words
A method for stimulating organogenesis of explants in a tissue culture medium comprising the step of: illuminating the explant during at least a portion of its differentiation stage with light having a predominate spectral emission at approximately 660nm.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein the donor plant is selected from the group consisting of lettuce, fir trees and pine trees.
Claim 4
Dependent Claim
19 words
The method of claim 1 wherein the explants are illuminated for a period of approximately 5 minutes per day.
Claim 5
Dependent Claim
21 words
The method of claim 1 wherein the light intensity is in the range of approximately 0.010 to 2.0 mW/cm 2 .
Claim 6
Dependent Claim
20 words
The method of claim 1 wherein the explants are only illuminated during approximately the second week of the culture period.
Claim 7
Independent Claim
89 words
A method for stimulating organogenesis of explants in a tissue culture medium comprising the steps of: placing an embryo of the donor plant in a culture medium; illuminating the cultured embryo with light having a predominate spectral emission at a wavelength of approximately 660nm to induce shoot formation; and illuminating the cultured embryo with light having a predominate spectral emission at a wavelength of approximately 740nm to stimulate root formation, an intervening period being provided between the two illuminations sufficiently long to prevent photoreversibility of the firstly stimulated effect.
Claim 8
Independent Claim
89 words
A method for stimulating organogenesis of explants in a tissue culture medium comprising the steps of: placing an embryo of the donor plant in a culture medium; illuminating the cultured embryo with light having a predominate spectral emission at a wavelength of approximately 660nm to induce shoot formation; and illuminating the cultured embryo with light having a predominate spectral emission at a wavelength of approximately 740nm to stimulate root formation, an intervening period being provided between the two illuminations sufficiently long to prevent photoreversibility of the firstly stimulated effect.
Claim 9
Dependent Claim
16 words
The method of claim 6 wherein the cultured embryo is initially illuminated with the 660nm light.
Claim 10
Dependent Claim
15 words
The method of claim 6 wherein the intervening period is from one to two weeks.
Claim 11
Independent Claim
54 words
A method for stimulating organogenesis of embryo explants in a tissue culture medium comprising the steps of: placing only the distal or cotyledon end of the embryo in the culture medium and illuminating the embryo during at least a portion of its differentiation stage with light having a predominate spectral emission of approximately 660nm.
Claim 12
Independent Claim
54 words
A method for stimulating organogenesis of embryo explants in a tissue culture medium comprising the steps of: placing only the distal or cotyledon end of the embryo in the culture medium and illuminating the embryo during at least a portion of its differentiation stage with light having a predominate spectral emission of approximately 660nm.
Claim 13
Dependent Claim
43 words
The method of claim 9 including the step of: illuminating the cultured embryo with light having at predominate spectral wavelength of 740nm, an intervening period being established between the two illumination periods to generally prevent photoreversibility of the firstly stimulated organ forming process.
Claim 14
Dependent Claim
24 words
The method of claim 9 wherein the distal or cotyledon end has been excised from the embryo prior to placement in the culture medium.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-05 | 1981-06-06 | 1981-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-05 | 1985-06-06 | 1985-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-05 | 1989-06-06 | 1989-12-06 | $7700.00 | Window Closed / Lapsed |