US4060457A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus for growing animal cells
Assignee: Individual
•
Filing: 1976-01-07
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Granted: 1977-11-29
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Expires: 1996-01-07
Abstract
Abstract An apparatus for growing animal cells of this invention comprises tubes with a hole near bottom of each tube and a variable angle tube holder. Cells may be attached and proliferated in the tubes which contain a nutrient medium, which can be removed simply from the tubes through holes by changing the angle of the tubes. Cell sheets in many tubes can be washed simply, rapidly and simultaneously.
Inventors
- 👤 Masahiko Iizuka
- 👤 Jiro Suzuki
- 👤 Sigeyasu Kobayashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:29
CPC Classifications
B; B01; B01L; B01L9/00; B01L9/06; C; C12; C12M; C12M23/00; C12M23/02; C12M23/04; C12M23/06; C12M23/12; C12M23/22; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
167 words
An apparatus for growing animal cells, said apparatus comprising: a. a plurality of small vessels, each said vessel having a hole in the lower portion thereof; b. a box reservoir; and c. vessel holder means including an upper panel and a lower panel mounted in said box reservoir, said upper panel and said lower panel each including holding means for cooperating with each other to hold each of said vessels, wherein at least one of said panels can be moved horizontally with respect to the other of said panels such that the angle of the axis of each said vessel when in said holder means can be set parallel to one another at 30°-60° on either side of the vertical by the horizontal movement of said panels with respect to one another, and wherein said hole is positioned in said vessel such that it is at the lowest point of said vessel when said vessel is tilted to one side of the vertical by said holder means.
Claim 2
Independent Claim
167 words
An apparatus for growing animal cells, said apparatus comprising: a. a plurality of small vessels, each said vessel having a hole in the lower portion thereof; b. a box reservoir; and c. vessel holder means including an upper panel and a lower panel mounted in said box reservoir, said upper panel and said lower panel each including holding means for cooperating with each other to hold each of said vessels, wherein at least one of said panels can be moved horizontally with respect to the other of said panels such that the angle of the axis of each said vessel when in said holder means can be set parallel to one another at 30°-60° on either side of the vertical by the horizontal movement of said panels with respect to one another, and wherein said hole is positioned in said vessel such that it is at the lowest point of said vessel when said vessel is tilted to one side of the vertical by said holder means.
Claim 3
Dependent Claim
15 words
The apparatus of claim 1 wherein said small vessel is tube having a circular bottom.
Claim 4
Dependent Claim
13 words
The apparatus of claim 1 wherein said small vessel is made of glass.
Claim 5
Dependent Claim
11 words
The apparatus of claim 1 wherein said box reservoir is transparent.
Claim 6
Dependent Claim
16 words
The apparatus of claim 1 wherein said box reservoir is in the shape of a dish.
Claim 7
Dependent Claim
20 words
The apparatus of claim 1 wherein said box reservoir has a groove at the side thereof for supporting said panels.
Claim 8
Dependent Claim
41 words
The apparatus of claim 1 wherein said box reservoir has two vertical walls parallel to the direction of movement of said panels and walls joining said two vertical walls which are tilted in the direction of tilting of said small vessels.
Claim 9
Dependent Claim
30 words
The apparatus of claim 1 wherein said box reservoir and upper and lower panels are transparent and said holding means comprises a plurality of small oval holes in said panels.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-11-28 | 1981-05-30 | 1981-11-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-11-28 | 1985-05-30 | 1985-11-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-11-28 | 1989-05-30 | 1989-11-29 | $7700.00 | Window Closed / Lapsed |