US4059491A
Expired / Lapsed
US
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Diluents for rubella virus hemagglutination-inhibition test
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1977-01-31
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Granted: 1977-11-22
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Expires: 1997-01-31
Abstract
Abstract In the rubella virus hemagglutination-inhibition test, the hemagglutination-inhibition antibody titers of the test sera can be determined with accuracy and high sensitivity by employing a novel diluent, which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a low concentration and which is free from calcium ion, for the dilution of the rubella virus hemagglutinating antigen and of the test sera.
Inventors
- 👤 Susumu Iwasa
- 👤 Isamu Yoshida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 12:49
CPC Classifications
H; H04; H04R; H04R1/00; H04R1/02; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/108331
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
51 words
A diluent for rubella virus hemagglutinating antigen and for rubella virus hemagglutination-inhibition test sera, which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.001 to about 0.01 and NaCl, the pH of which is in the range of about 6.0 to about 7.5, and which is free from calcium ion.
Claim 2
Dependent Claim
51 words
A diluent for rubella virus hemagglutinating antigen and for rubella virus hemagglutination-inhibition test sera, which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.001 to about 0.01 and NaCl, the pH of which is in the range of about 6.0 to about 7.5, and which is free from calcium ion.
Claim 3
Dependent Claim
24 words
A diluent as claimed in claim 1, wherein the molar concentration of N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid is in the range of about 0.004 to about 0.006.
Claim 4
Dependent Claim
19 words
A diluent as claimed in claim 1, which is in the pH range of about 6.0 to about 6.5.
Claim 5
Dependent Claim
11 words
A diluent as claimed in claim 1, which further contains albumin.
Claim 6
Dependent Claim
13 words
A diluent as claimed in claim 4, wherein the albumin is serum albumin.
Claim 7
Dependent Claim
21 words
A diluent as claimed in claim 4, wherein the albumin is in a concentration from about 0.05 to about 1 percent.
Claim 8
Dependent Claim
23 words
A diluent as claimed in claim 1, wherein the molar concentration of NaCl is in the range of about 0.05 to about 0.3.
Claim 9
Independent Claim
134 words
A method for rubella virus hemagglutination -inhibition test, which comprises admixing a test serum previously diluted with a diluent which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.001 to about 0.01 and NaCl, the pH of which is in the range of about 6.0 to about 7.5 and which is free from calcium ion with rubella virus hemagglutinating antigen previously diluted with the said diluent and, then, admixing the resultant mixture with a suspension of erythrocytes previously diluted with a diluent which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.01 to about 0.05, CaCl 2 in a molar concentration from about 0.0005 to about 0.01 and NaCl and the pH of which is in the range of about 6.0 to about 6.5 to assess agglutination or nonagglutination of the erythrocytes.
Claim 10
Independent Claim
134 words
A method for rubella virus hemagglutination -inhibition test, which comprises admixing a test serum previously diluted with a diluent which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.001 to about 0.01 and NaCl, the pH of which is in the range of about 6.0 to about 7.5 and which is free from calcium ion with rubella virus hemagglutinating antigen previously diluted with the said diluent and, then, admixing the resultant mixture with a suspension of erythrocytes previously diluted with a diluent which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.01 to about 0.05, CaCl 2 in a molar concentration from about 0.0005 to about 0.01 and NaCl and the pH of which is in the range of about 6.0 to about 6.5 to assess agglutination or nonagglutination of the erythrocytes.
Claim 11
Dependent Claim
22 words
A method as claimed in claim 8, wherein the diluent for rubella virus hemagglutinating antigen and for test serum further contains albumin.
Claim 12
Dependent Claim
15 words
A method as claimed in claim 8, wherein the diluent for erythrocytes further contains gelatin.
Claim 13
Dependent Claim
14 words
A method as claimed in claim 8, wherein the test serum is human serum.
Claim 14
Dependent Claim
13 words
A method as claimed in claim 8, wherein the erythrocytes are fowl erythrocytes.
Claim 15
Dependent Claim
12 words
A method as claimed in claim 12, wherein the fowl is chick.
Claim 16
Dependent Claim
13 words
A method as claimed in claim 8, wherein the erythrocytes are fixed erythrocytes.
Claim 17
Dependent Claim
14 words
A diluent as claimed in claim 3, which has a pH of about 6.2.
Claim 18
Dependent Claim
23 words
A diluent as claimed in claim 7, wherein the molar concentration of NaCl is in the range of about 0.14 to about 0.15.
References Cited (Backward Citations)
US3516794A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-11-21 | 1981-05-23 | 1981-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-11-21 | 1985-05-23 | 1985-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-11-21 | 1989-05-23 | 1989-11-22 | $7700.00 | Window Closed / Lapsed |