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US4059489A
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Production of glucose isomerase
Assignee: Imperial Chemical Industries Ltd
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Filing: 1975-07-11
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Granted: 1977-11-22
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Expires: 1995-07-11
Abstract
Abstract A process for the continuous production of glucose isomerase by maintaining a nutrient in the culture medium at a concentration which is limiting to growth. Preferably the growth limiting nutrient is the carbon source. The invention also covers a process for isomerizing glucose to fructose using continuously prepared glucose isomerase. Important conditions for culturing the glucose isomerase producing microorganism are the dissolved oxygen tension and dilution rate.
Inventors
- 👤 John Laurence Meers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 09:37
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/90; C12N9/92; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/867; Y10S435/869; Y10S435/886; Y10S435/887
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
63 words
A process for producing glucose isomerase enzyme by continuously cultivating a glucose isomerase-producing microorganism in a culture medium comprising a source of assimilable carbon and inorganic nutrients under conditions suitable for production of said enzyme and continuously recovering said enzyme wherein the concentration of a nutrient source in the culture medium is maintained at a level such as to be limiting to growth.
Claim 2
Dependent Claim
63 words
A process for producing glucose isomerase enzyme by continuously cultivating a glucose isomerase-producing microorganism in a culture medium comprising a source of assimilable carbon and inorganic nutrients under conditions suitable for production of said enzyme and continuously recovering said enzyme wherein the concentration of a nutrient source in the culture medium is maintained at a level such as to be limiting to growth.
Claim 3
Dependent Claim
30 words
A process according to claim 1 wherein the nutrient source, whose concentration in the culture medium is maintained at a level which is limiting to growth, is the carbon source.
Claim 4
Dependent Claim
78 words
A process according to claim 1 wherein sources of carbon, nitrogen, phosphorus, magnesium, sulphur, potassium and organic nitrogen are added to the culture medium in concentrations (% w/w of the source compound) within the ranges: Carbon source -- 0.05 to 10% Nitrogen source -- 0.001 to 3% Phosphorus source -- 0.01 to 0.5% Magnesium source -- 0.001 to 0.2% Sulphur source -- 0.01 to 0.25% Potassium source -- 0.01 to 0.25% Organic nitrogen source -- 0.01 to 5%
Claim 5
Dependent Claim
26 words
A process according to claim 1 wherein the microorganism is a strain of a genus selected from the group consisting of Streptomyces, Arthrobacter, Mycobacterium and Curtobacterium.
Claim 6
Dependent Claim
20 words
A process according to claim 1 wherein the carbon source is selected from the group consisting of glucose and xylose.
Claim 7
Dependent Claim
22 words
A process according to claim 1 wherein the culture medium is maintained at a temperature within the range 20° to 55° C.
Claim 8
Dependent Claim
21 words
A process according to claim 1 wherein the pH of the culture medium is maintained within the range 6.0 to 8.0.
Claim 9
Dependent Claim
27 words
A process according to claim 1 wherein the dissolved oxygen tension (DOT) within the culture medium is controlled within the range 0 to 150 mms of mercury.
Claim 10
Dependent Claim
23 words
A process according to claim 8 wherein the dissolved oxygen tension (DOT) is controlled within the range 30 to 100 mms of mercury.
Claim 11
Dependent Claim
20 words
A process according to claim 1 wherein the dilution rate is within the range 0.05 to 0.4 hrs -1 .
Claim 12
Dependent Claim
79 words
A process which comprises isomerizing glucose to fructose wherein there is added in said isomerizing a glucose isomerase enzyme which has been produced by continuously cultivating a glucose isomerase-producing microorganism in a culture medium comprising a source of assimilable carbon and inorganic nutrients under conditions suitable for production of said enzyme and continuously recovering said enzyme wherein the concentration of a nutrient source in the culture medium is maintained at a level such as to be limiting to growth.
Claim 13
Dependent Claim
79 words
A process which comprises isomerizing glucose to fructose wherein there is added in said isomerizing a glucose isomerase enzyme which has been produced by continuously cultivating a glucose isomerase-producing microorganism in a culture medium comprising a source of assimilable carbon and inorganic nutrients under conditions suitable for production of said enzyme and continuously recovering said enzyme wherein the concentration of a nutrient source in the culture medium is maintained at a level such as to be limiting to growth.
Claim 14
Dependent Claim
36 words
A process according to claim 11, wherein, during the production of the enzyme, the nutrient source, whose concentration in the culture medium is maintained at a level which is limiting to growth, is the carbon source.
Claim 15
Dependent Claim
21 words
A process according to claim 11 wherein during the isomerization the temperature is maintained within the range 20° to 90° C.
Claim 16
Dependent Claim
23 words
A process according to claim 11 wherein the pH of the glucose-containing liquor undergoing isomerization is maintained within the range 5 to 9.
Claim 17
Dependent Claim
24 words
A process according to claim 11 wherein ions selected from the group consisting of magnesium, cobalt and manganese ions are present during the isomerization.
References Cited (Backward Citations)
US2822319A
Cited
US3396083A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-11-21 | 1981-05-23 | 1981-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-11-21 | 1985-05-23 | 1985-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-11-21 | 1989-05-23 | 1989-11-22 | $7700.00 | Window Closed / Lapsed |