US4053582A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Attenuated fowl pox virus preparation for the treatment of infectious diseases, method for the manufacture thereof, and its use
Assignee: Individual
•
Filing: 1975-07-28
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Granted: 1977-10-11
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Expires: 1995-07-28
Abstract
Abstract Preparation for the treatment of interferon-sensitive infectious diseases for use in human and veterinary medicine, characterized by the fact that it contains as active principle a fowl pox virus which has been attenuated by 420 to 800 cell culture passages, optionally together with a pharmaceutical excipient or adjuvant, preferably containing a sufficient number of virus units so as to provide a total of 10 8 to 10 10 virus units per treatment involving 2 to 6 administrations thereof, method for the production thereof, method of producing pharmaceutical forms thereof, the pharmaceutical forms themselves, and a method of growing an effective fowl pox virus for use in the preparations of the invention.
Inventors
- 👤 Helmut Anton Stickl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 20:34
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/275; A61K39/285; A61K35/00; A61K35/66; A61K35/76; C; C12; C12N; C12N2710/00; C12N2710/00011; C12N2710/24011; C12N2710/24111; C12N2710/24132; C12N2710/24134; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
51 words
Preparation useful for the treatment of interferonsensitive infectious diseases for use in human and veterinary medicine, characterized by the fact that it contains as active principle an effective amount of fowl pox virus, which has been attenuated by 420 to 800 cell culture passages, together with a pharmaceutical excipient or adjuvant.
Claim 2
Dependent Claim
51 words
Preparation useful for the treatment of interferonsensitive infectious diseases for use in human and veterinary medicine, characterized by the fact that it contains as active principle an effective amount of fowl pox virus, which has been attenuated by 420 to 800 cell culture passages, together with a pharmaceutical excipient or adjuvant.
Claim 3
Dependent Claim
24 words
Preparation according to claim 1, characterized by the fact that it is present in an isotonic salt solution, and may contain a color indicator.
Claim 4
Dependent Claim
13 words
Preparation according to claim 2, wherein the isotonic salt solution is Ringer's solution.
Claim 5
Dependent Claim
12 words
Preparation according to claim 2, wherein the color indicator is phenol red.
Claim 6
Dependent Claim
41 words
Preparation according to claim 1, characterized by the fact that it contains a sufficient number of virus units so as to provide a total of 10 8 to 10 10 virus units per treatment, each treatment involving 2 to 6 doses.
Claim 7
Dependent Claim
29 words
A method for active interferonization of humans against infectious diseases characterized by administering an effective amount of a preparation in accordance with claim 1 orally, nasally, intracutaneously, or intramuscularly.
Claim 8
Dependent Claim
29 words
A method for active interferonization of mammals against infectious diseases characterized by administering an effective amount of a preparation in accordance with claim 1 orally, nasally, intracutaneously, or intramuscularly.
Claim 9
Independent Claim
86 words
A method of producing a preparation useful for the endogenic treatment of interferon-sensitive infectious diseases for use in human and veterinary medicine, characterized by (1) applying an original fowl pox virus to a cell culture of embryonated fowl eggs, (2) attenuating it by about 420 to 800 cell culture passages, (3) harvesting the medium with the remaining cells, (4) digesting the cells, (5) separating the coarse cell components from the virus, and (6) recovering the virus from the virus-containing supernatant material by centrifuging, precipitation, or freezedrying.
Claim 10
Independent Claim
86 words
A method of producing a preparation useful for the endogenic treatment of interferon-sensitive infectious diseases for use in human and veterinary medicine, characterized by (1) applying an original fowl pox virus to a cell culture of embryonated fowl eggs, (2) attenuating it by about 420 to 800 cell culture passages, (3) harvesting the medium with the remaining cells, (4) digesting the cells, (5) separating the coarse cell components from the virus, and (6) recovering the virus from the virus-containing supernatant material by centrifuging, precipitation, or freezedrying.
Claim 11
Dependent Claim
35 words
A method according to claim 8, wherein the recovered virus is combined with a pharmaceutical excipient or adjuvant into the form of a pharmaceutical formulation which can be administered locally, orally, nasally, intracutaneously, or intramuscularly.
Claim 12
Dependent Claim
16 words
A method according to claim 8, wherein, the attenuation includes the step of clonizing the culture.
Claim 13
Dependent Claim
20 words
A method according to claim 8, characterized by the fact that the nutrient medium used comprises fowl embryo fibroblast cells.
Claim 14
Dependent Claim
17 words
The method according to claim 8, wherein the cell culture is fibroblast cells from embryonated hens' eggs.
Claim 15
Dependent Claim
23 words
The method according to claim 12, wherein the cell culture growth medium is 80% Earle's Solution, 10% beef embryo serum, and 10% lactalbumin.
Claim 16
Dependent Claim
38 words
The method according to claim 8, wherein the fowl pox virus is passaged 429 times serially followed by a triple clonization to produce a seed virus for production and a starting virus for further cell passages designated "Mayr-Stickl-Avipox-Interferon-Inducer".
References Cited (Backward Citations)
US3429965A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-10-10 | 1981-04-11 | 1981-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-10-10 | 1985-04-11 | 1985-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-10-10 | 1989-04-11 | 1989-10-11 | $7700.00 | Window Closed / Lapsed |