US4051314A
Expired / Lapsed
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Polysaccharides and method for producing same
Assignee: Kureha Corp
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Filing: 1974-10-11
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Granted: 1977-09-27
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Expires: 1994-10-11
Abstract
Abstract Polysaccharides are produced by purifying a liquid extract of a mycelium of a strain of fungus species belonging to the class Basidiomycetes or the filtered broth of a cultured medium in which a mycelium of a selected strain of Basidiomycetes has been incubated; the polysaccharides exhibiting an anticarcinogenic activity in mice.
Inventors
- 👤 Shigeto Ohtsuka
- 👤 Saburo Ueno
- 👤 Chikao Yoshikumi
- 👤 Fumio Hirose
- 👤 Yoshio Ohmura
- 👤 Toshihiko Wada
- 👤 Takayoshi Fujii
- 👤 Eiichi Takahashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 18:02
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/04; A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A61K36/00; A61K36/06; A61K36/07; A61K36/18; A61K36/88; A61K36/899; C08; C08B; C08B37/00; C08B37/006; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/911
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
101 words
A process for the production of a polysaccharide comprising forming a culture medium or a liquid extract of mycelium of a species of fungi belonging to the class Basidiomycetes and selected from the group consisting of Coriolus versicolor, Coriolus conchifer, Coriolus pargamenus, Coriolus hirsutus, Coriolus biformis, Coriolus consors and Coriolus pubescens, separating said polysaccharide from the free proteins, and other impurities contained in said culture medium or liquid extract and purifying said polysaccharide to produce a purified polysaccharide having an acute toxicity (LD50) in mice of more than 20 g/kg for oral administration and more than 100 mg/kg for subcutaneous injection.
Claim 2
Dependent Claim
101 words
A process for the production of a polysaccharide comprising forming a culture medium or a liquid extract of mycelium of a species of fungi belonging to the class Basidiomycetes and selected from the group consisting of Coriolus versicolor, Coriolus conchifer, Coriolus pargamenus, Coriolus hirsutus, Coriolus biformis, Coriolus consors and Coriolus pubescens, separating said polysaccharide from the free proteins, and other impurities contained in said culture medium or liquid extract and purifying said polysaccharide to produce a purified polysaccharide having an acute toxicity (LD50) in mice of more than 20 g/kg for oral administration and more than 100 mg/kg for subcutaneous injection.
Claim 3
Dependent Claim
11 words
The process of claim 1 wherein said species is Coriolus versicolor.
Claim 4
Dependent Claim
9 words
The polysaccharide produced by the process of claim 1.
Claim 5
Dependent Claim
100 words
A polysaccharide, which in the form of a hydrolyzate, gives positive ninhydrin, anisaldehyde, molisch, anthrone, tryptophane-sulfonic acid, chromotropic acid-sulfuric acid, carbazole cysteine-sulfonic acid, aniline-hydrochloric acid, resorcinol-hydrochloric acid, tollens and thioglycol-sulfuric acid reactions but negative ferric chloride and fehling reactions, said polysaccharide being produced by the process comprising forming a culture medium or a liquid extract of mycelium of a species of fungi belonging to the class Basidiomycetes and selected from the group consisting of Coriolus versicolor, Coriolus conchifer, Coriolus pargamenus, Coriolus hirsutus, Coriolus biformis, Coriolus consors and Coriolus pubescens, and separating the polysaccharide from said culture medium or liquid extract.
Claim 6
Dependent Claim
100 words
A polysaccharide, which in the form of a hydrolyzate, gives positive ninhydrin, anisaldehyde, molisch, anthrone, tryptophane-sulfonic acid, chromotropic acid-sulfuric acid, carbazole cysteine-sulfonic acid, aniline-hydrochloric acid, resorcinol-hydrochloric acid, tollens and thioglycol-sulfuric acid reactions but negative ferric chloride and fehling reactions, said polysaccharide being produced by the process comprising forming a culture medium or a liquid extract of mycelium of a species of fungi belonging to the class Basidiomycetes and selected from the group consisting of Coriolus versicolor, Coriolus conchifer, Coriolus pargamenus, Coriolus hirsutus, Coriolus biformis, Coriolus consors and Coriolus pubescens, and separating the polysaccharide from said culture medium or liquid extract.
Claim 7
Dependent Claim
116 words
A polysaccharide having a molecular weight within the range of 1.0-1.9 × 100000, as determined by gel filtration, and which, in the form of a hydrolyzate, gives positive ninhydrin, anisaldehyde, molisch, anthrone, tryptophane-sulfonic acid, chromotropic acid-sulfuric acid, carbazole-cysteine-sulfuric acid, aniline-hydrochloric acid, resorcinol-hydrochloric acid, tollens and thioglycol-sulfuric acid reactions but negative ferric chloride and fehling reactions, said polysaccharide being produced by the process comprising forming a culture medium or a liquid extract of mycelium of a species of fungi belonging to the class Basidiomycetes and selected from the group consisting of Coriolus versicolor, Coriolus conchifer, Coriolus pargamenus, Coriolus hirsutus, Coriolus biformis, Coriolus consors and Coriolus pubescens, and separating the polysaccharide from said culture medium or liquid extract.
Claim 8
Dependent Claim
116 words
A polysaccharide having a molecular weight within the range of 1.0-1.9 × 100000, as determined by gel filtration, and which, in the form of a hydrolyzate, gives positive ninhydrin, anisaldehyde, molisch, anthrone, tryptophane-sulfonic acid, chromotropic acid-sulfuric acid, carbazole-cysteine-sulfuric acid, aniline-hydrochloric acid, resorcinol-hydrochloric acid, tollens and thioglycol-sulfuric acid reactions but negative ferric chloride and fehling reactions, said polysaccharide being produced by the process comprising forming a culture medium or a liquid extract of mycelium of a species of fungi belonging to the class Basidiomycetes and selected from the group consisting of Coriolus versicolor, Coriolus conchifer, Coriolus pargamenus, Coriolus hirsutus, Coriolus biformis, Coriolus consors and Coriolus pubescens, and separating the polysaccharide from said culture medium or liquid extract.
Claim 9
Dependent Claim
59 words
The polysaccharide produced by the process comprising forming a culture medium or liquid extract of mycelium of a species of fungi belonging to the class Basidiomycetes and selected from the group consisting of Coriolus versicolor, Coriolus conchifer, Coriolus pargamenus, Coriolus hirsutus, Coriolus biformis, Coriolus consors and Coriolus pubescens, and separating the polysaccharide from said culture medium or liquid extract.
Claim 10
Dependent Claim
59 words
The polysaccharide produced by the process comprising forming a culture medium or liquid extract of mycelium of a species of fungi belonging to the class Basidiomycetes and selected from the group consisting of Coriolus versicolor, Coriolus conchifer, Coriolus pargamenus, Coriolus hirsutus, Coriolus biformis, Coriolus consors and Coriolus pubescens, and separating the polysaccharide from said culture medium or liquid extract.
Claim 11
Dependent Claim
30 words
The polysaccharide produced by the process comprising forming a culture medium or liquid extract of mycelium of Coriolus versicolor, and separating the polysaccharide from said culture medium or liquid extract.
Claim 12
Dependent Claim
30 words
The polysaccharide produced by the process comprising forming a culture medium or liquid extract of mycelium of Coriolus versicolor, and separating the polysaccharide from said culture medium or liquid extract.
Claim 13
Dependent Claim
9 words
The polysaccharide produced by the process of claim 2.
Claim 14
Dependent Claim
42 words
The polysaccharide of claim 8, which in the form of a hydrolyzate, gives positive ninhydrin, anisaldehyde, molisch, anthrone, tryptophane-sulfonic acid, chromotropic acid-sulfuric acid, carbazole cysteine-sulfonic acid, anilinehydrochloric acid, resorcinol-hydrochloric acid, tollens and thioglycol-sulfuric acid reactions but negative ferric chloride and fehline reactions.
References Cited (Backward Citations)
US3301848A
Cited
US3759896A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-26 | 1981-03-28 | 1981-09-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-26 | 1985-03-28 | 1985-09-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-26 | 1989-03-28 | 1989-09-27 | $7700.00 | Window Closed / Lapsed |