Patent US4293949A successfully refreshed from Google Patents.
US4048014A
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Method for obtaining urokinase
Assignee: Resonac Corp
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Filing: 1976-07-06
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Granted: 1977-09-13
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Expires: 1996-07-06
Abstract
Abstract A method for obtaining urokinase which comprises steps of contacting a partially purified urokinase-containing solution with diatomaceous earth at a pH range of 4.0 - 8.0, washing said diatomaceous earth adsorbing urokinase thereon with a buffer solution of pH 6.5 - 9.0 and eluting the urokinase from the adsorbate.
Inventors
- 👤 Masaharu Urakawa
- 👤 Hiroshi Sumiyama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 10:53
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
51 words
A method for obtaining urokinase which comprises contacting a partially purified urokinase-containing solution with diatomaceous earth at a pH range of 4.0 - 8.0, washing said diatomaceous earth adsorbing urokinase thereon with a buffer solution of pH 6.5 - 9.0 and eluting the urokinase from the adsorbate with a basic solution.
Claim 2
Independent Claim
51 words
A method for obtaining urokinase which comprises contacting a partially purified urokinase-containing solution with diatomaceous earth at a pH range of 4.0 - 8.0, washing said diatomaceous earth adsorbing urokinase thereon with a buffer solution of pH 6.5 - 9.0 and eluting the urokinase from the adsorbate with a basic solution.
Claim 3
Dependent Claim
34 words
A method as claimed in claim 1, wherein said partially purified urokinase-containing solution is obtained by removing the precipitates produced by adjusting the pH of human urine to above 7.5 with a basic solution.
Claim 4
Dependent Claim
38 words
A method as claimed in claim 1, wherein said contact operation is carried out at a pH range of 5.0 - 7.0 and said washing operation is carried out with a buffer solution of pH 7.5 - 8.5.
Claim 5
Dependent Claim
20 words
A method as claimed in claim 1, wherein said buffer solution of pH 6.5 -9.5 is a phosphate buffer solution.
Claim 6
Dependent Claim
20 words
A method as claimed in claim 1, wherein said basic solution is an aqueous solution of ammonia or sodium carbonate.
Claim 7
Dependent Claim
23 words
A method as claimed in claim 5, wherein said aqueous solution of ammonia is an aqueous solution containing above 4 % of ammonia.
References Cited (Backward Citations)
US3723252A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-12 | 1981-03-14 | 1981-09-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-12 | 1985-03-14 | 1985-09-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-12 | 1989-03-14 | 1989-09-13 | $7700.00 | Window Closed / Lapsed |