US4046921A
Expired / Lapsed
US
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Process for cultivating microorganisms by means of fluidized bed
Assignee: Kikkoman Corp
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Filing: 1976-02-04
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Granted: 1977-09-06
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Expires: 1996-02-04
Abstract
Abstract A process for cultivating microorganisms by means of fluidized bed characterized by cultivating a microorganism on a solid culture medium while mixing the solid culture medium in the fluidized bed with an upward gas stream and supplying an atomized liquid into the culture. According to this process, a cultivated product being high in enzymatic activity and quality is obtained with a high efficiency.
Inventors
- 👤 Takeshi Akao
- 👤 Toshio Sakasai
- 👤 Yoshikazu Matsuyama
- 👤 Yukio Kasuga
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 18:25
CPC Classifications
C; C12; C12M; C12M25/00; C12M25/16; C12M25/20; A; A23; A23L; A23L27/00; A23L27/50; B; B01; B01J; B01J8/00; B01J8/18; B01J8/24; B01J8/38; B01J8/382; C12M21/00; C12M21/16; C12M27/00; C12M27/02; C12M29/00; C12M29/06
Claims Analysis
Total Claims: 42
Claim 1
Independent Claim
49 words
A process for producing a koji for use in enzymic preparations by means of fluidized bed comprising cultivating a microorganism on a solid culture medium while mixing the solid culture medium in a fluidized bed with an upward gas stream and supplying an atomized liquid into the solid culture.
Claim 2
Dependent Claim
49 words
A process for producing a koji for use in enzymic preparations by means of fluidized bed comprising cultivating a microorganism on a solid culture medium while mixing the solid culture medium in a fluidized bed with an upward gas stream and supplying an atomized liquid into the solid culture.
Claim 3
Dependent Claim
34 words
A process according to claim 1, wherein said atomized liquid is an aqueous liquid and the quantity of the liquid supplied to the solid culture is in the range of 10 to 250% (v/w).
Claim 4
Dependent Claim
26 words
A process according to claim 1, wherein the quantity of the liquid supplied to the solid culture is in the range of 15 to 150% (v/w).
Claim 5
Dependent Claim
45 words
A process according to claim 1, wherein the starting material of the solid culture is at least one member selected from the group consisting of globular soy bean, defatted soy bean, wheat gluten, corn gluten, concentrated proteins, isolated proteins, wheat, bran, barley, rice and starch.
Claim 6
Dependent Claim
27 words
A process according to claim 1, wherein at least one member selected from the group consisting of inorganic salts and vitamins is added to the solid culture.
Claim 7
Dependent Claim
24 words
A process according to claim 1, wherein the starting material of said culture medium has an average particle diameter of 0.07 to 1.0 mm.
Claim 8
Dependent Claim
23 words
A process according to claim 1, wherein the starting material of said culture has an average particle diameter of 0.1 to 0.5 mm.
Claim 9
Dependent Claim
26 words
A process according to claim 1, wherein the gas is at least one member selected from the group consisting of air, oxygen, carbon dioxide and nitrogen.
Claim 10
Dependent Claim
14 words
A process according to claim 1, wherein the gas is incorporated with sulfur dioxide.
Claim 11
Dependent Claim
21 words
A process according to claim 1, wherein the amount of gas introduced is in the range of 15 to 70 cm/sec.
Claim 12
Dependent Claim
45 words
A process according to claim 1, wherein the liquid is either water itself or water incorporated with at least one member selected from the group consisting of minerals, vitamins, amino acids, the culture itself, organic salts, inorganic salts, fungicides, bactericides, static agents and bacteria-controlling agents.
Claim 13
Dependent Claim
34 words
A process according to claim 1, wherein the liquid is supplied to the solid culture so as to maintain the water content of the solid culture in the range of 15 to 70% (v/w).
Claim 14
Dependent Claim
21 words
A process according to claim 1, wherein the cultivation is carried out at a temperature of 25° C. to 45° C.
Claim 15
Dependent Claim
17 words
A process according to claim 1, wherein the cultivation is carried out for 20 to 80 hours.
Claim 16
Dependent Claim
17 words
A process according to claim 1, wherein the microorganism is a mould, a yeast or a bacterium.
Claim 17
Dependent Claim
12 words
A process according to claim 1, wherein the microorganism is a mould.
Claim 18
Dependent Claim
21 words
A process according to claim 16, wherein the mould is of long stalk-strain belonging to the genus Rhizopus, Mucor or Aspergillus.
Claim 19
Dependent Claim
126 words
A process according to claim 1, wherein the microorganism is Aspergillus soyae ATCC 20387 (FERM-P No. 504), Aspergillus soyae ATCC 20388 (FERM-P No. 505), Aspergillus oryzae ATCC 20386, Aspergillus oryzae IAM 2742, Aspergillus saitoi R-3813 (ATCC 14332), Aspergillus niger NRRL 337, Aspergillus niger NRRL 330, Aspergillus inuii ATCC 14334, Aspergillus usamii ATCC 14331, Aspergillus awamori ATCC 14335, Rhizopus oligosporous NRRL 2710, Rhizopus japonicus IAM 6002, Rhizopus formosaensis IAM 6245, Mucor javanicus IAM 6108, Mucor javanicus HUT 1168, Saccharomyces rouxii IFO 0495, Saccharomyces rouxii IFO 0505, Saccharomyces rouxii IFO 0506, Saccharomyces rouxii IFO 0510, Saccharomyces rouxii IFO 0513, Saccharomyces rouxii IFO 0517, Saccharomyces rouxii IFO 0570, Saccharomyces rouxii OUT 7134, Saccharomyces rouxii OUT 7135, Saccharomyces rouxii OUT 7136, Pediococcus halophilus ATCC 13624, or Pediococcus soyae IAM 1681.
Claim 20
Dependent Claim
46 words
A process for producing a soy sauce koji by means of fluidized bed comprising cultivating a microorganism on a solid culture medium while mixing the solid culture medium in a fluidized bed with an upward gas stream and supplying an atomized liquid into the solid culture.
Claim 21
Dependent Claim
46 words
A process for producing a soy sauce koji by means of fluidized bed comprising cultivating a microorganism on a solid culture medium while mixing the solid culture medium in a fluidized bed with an upward gas stream and supplying an atomized liquid into the solid culture.
Claim 22
Dependent Claim
34 words
A process according to claim 19, wherein said atomized liquid is an aqueous liquid and the quantity of the liquid supplied to the solid culture is in the range of 10 to 250% (v/w).
Claim 23
Dependent Claim
26 words
A process according to claim 19, wherein the quantity of the liquid supplied to the solid culture is in the range of 15 to 150% (v/w).
Claim 24
Dependent Claim
45 words
A process according to claim 19, wherein the starting material of the solid culture is at least one member selected from the group consisting of globular soy bean, defatted soy bean, wheat gluten, corn gluten, concentrated proteins, isolated proteins, wheat, bran, barley, rice and starch.
Claim 25
Dependent Claim
27 words
A process according to claim 19, wherein at least one member selected from the group consisting of inorganic salts and vitamins is added to the solid culture.
Claim 26
Dependent Claim
24 words
A process according to claim 19, wherein the starting material of said culture medium has an average particle diameter of 0.07 to 1.0 mm.
Claim 27
Dependent Claim
24 words
A process according to claim 19, wherein the starting material of said culture medium has an average particle diameter of 0.1 to 0.5 mm.
Claim 28
Dependent Claim
26 words
A process according to claim 19, wherein the gas is at least one member selected from the group consisting of air, oxygen, carbon dioxide and nitrogen.
Claim 29
Dependent Claim
14 words
A process according to claim 19, wherein the gas is incorporated with sulfur dioxide.
Claim 30
Dependent Claim
21 words
A process according to claim 19, wherein the amount of gas introduced is in the range of 15 to 70 cm/sec.
Claim 31
Dependent Claim
45 words
A process according to claim 19, wherein the liquid is either water itself or water incorporated with at least one member selected from the group consisting of minerals, vitamins, amino acids, the culture itself, organic salts, inorganic salts, fungicides bactericides, static agents and bacteria-controlling agents.
Claim 32
Dependent Claim
34 words
A process according to claim 19, wherein the liquid is supplied to the solid culture so as to maintain the water content of the solid culture in the range of 15 to 70% (v/w).
Claim 33
Dependent Claim
21 words
A process according to claim 19, wherein the cultivation is carried out at a temperature of 25° C. to 45° C.
Claim 34
Dependent Claim
17 words
A process according to claim 19, wherein the cultivation is carried out for 20 to 80 hours.
Claim 35
Dependent Claim
17 words
A process according to claim 19, wherein the microorganism is a mould, a yeast or a bacteriaum.
Claim 36
Dependent Claim
12 words
A process according to claim 19, wherein the microorganism is a mould.
Claim 37
Dependent Claim
18 words
A process according to claim 33, wherein the mould is of long stalk-strain belonging to the genus Aspergillus.
Claim 38
Dependent Claim
106 words
A process according to claim 19, wherein the microorganism is Aspergillus soyae ATCC 20387 (FERM-P No. 504), Aspergillus soyae ATCC 20388 (FERM-P No. 505), Aspergillus oryzae ATCC 20386, Aspergillus oryzae IAM 2742, Aspergillus saitoi R-3813 (ATCC 14332), Aspergillus niger NRRL 337, Aspergillus niger NRRL 330, Aspergillus inuii ATCC 14334, Aspergillus usamii ATCC 14331, Aspergillus awamori ATCC 14335, Saccharomyces rouxii IFO 0495, Saccharomyces rouxii IFO 0505, Saccharomyces rouxii IFO 0506, Saccharomyces rouxii IFO 0510, Saccharomyces rouxii IFO 0513, Saccharomyces rouxii IFO 0517, Saccharomyces rouxii IFO 0570, Saccharomyces rouxii OUT 7134, Saccharomyces rouxii OUT 7135, Saccharomyces rouxii OUT 7136, Pediococcus halophilus ATCC 13624, or Pediococcus soyae IAM 1681.
Claim 39
Dependent Claim
44 words
A process for producing a Miso-koji by means of fluidized bed comprising cultivating a microorganism on a solid culture medium while mixing the solid culture medium in a fluidized bed with an upward gas stream and supplying an atomized liquid into the solid culture.
Claim 40
Dependent Claim
44 words
A process for producing a Miso-koji by means of fluidized bed comprising cultivating a microorganism on a solid culture medium while mixing the solid culture medium in a fluidized bed with an upward gas stream and supplying an atomized liquid into the solid culture.
Claim 41
Dependent Claim
106 words
A process according to claim 37, wherein the microorganism is Aspergillus soyae ATCC 20387 (FERM-P No. 504), Aspergillus soyae ATCC 20388 (FERM-P No. 505), Aspergillus oryzae ATCC 20386, Aspergillus oryzae IAM 2742, Aspergillus saitoi R-3813 (ATCC 14332), Aspergillus niger NRRL 337, Aspergillus niger NRRL 330, Aspergillus inuii ATCC 14334, Aspergillus usamii ATCC 14331, Aspergillus awamori ATCC 14335, Saccharomyces rouxii IFO 0495, Saccharomyces rouxii IFO 0505, Saccharomyces rouxii IFO 0506, Saccharomyces rouxii IFO 0510, Saccharomyces rouxii IFO 0513, Saccharomyces rouxii IFO 0517, Saccharomyces rouxii IFO 0570, Saccharomyces rouxii OUT 7134, Saccharomyces rouxii OUT 7135, Saccharomyces rouxii OUT 7136, Pediococcus halophilus ATCC 13624, or Pediococcus soyae IAM 1681.
Claim 42
Dependent Claim
32 words
A process according to claim 38 wherein said atomized liquid is an aqueous liquid and the quantity of liquid supplied to the culture is in the range of 10 to 250% (v/w).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-05 | 1981-03-07 | 1981-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-05 | 1985-03-07 | 1985-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-05 | 1989-03-07 | 1989-09-06 | $7700.00 | Window Closed / Lapsed |