US4046635A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for detecting enzymes capable of digesting fibrinogen or fibrin
Assignee: McGill University
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Filing: 1975-08-27
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Granted: 1977-09-06
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Expires: 1995-08-27
Abstract
Abstract A method for detecting and measuring the activity of enzymes capable of digesting fibrinogen or fibrin and for detecting and measuring the activity of enzyme activators and enzyme inhibitors of said enzymes, by incubating an unknown enzyme sample in a humid labelled fibrinogen or labelled fibrin coated test apparatus, isolating the labelled degradation products of fibrinogen or fibrin released by the unknown enzyme sample and measuring the labelled degradation products of fibrinogen released by the unknown sample.
Inventors
- 👤 Leonard Arthur Moroz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/56; G; G01; G01N; G01N2333/00; G01N2333/435; G01N2333/745; G01N2333/75; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/968; G01N2333/972; G01N2333/9723; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
177 words
A method for detecting and measuring enzymes capable of digesting fibrinogen and for detecting and measuring activators and inhibitors of enzymes capable of digesting fibrinogen which comprises: a. coating at room temperature a water-insoluble polymer test apparatus with a buffered solution of a radioactive tracer labelled enzyme-free human fibrinogen to produce a moist coating; b. reacting said moist coating with a thrombin solution thereby converting the labelled fibrinogen to labelled fibrin; c. contacting said coating of radioactively labelled fibrin with a sample of the unknown enzyme or a mixture of a known enzyme capable of digesting fibrin and the unknown enzyme activator or a mixture of a known enzyme capable of digesting fibrin and the unknown enzyme inhibitor; d. incubating said sample in contact with said coating; e. washing the test apparatus with a buffered solution and recovering the solution containing the radioactively digested products released by said sample; f. counting radiation emitted by said digested and released fibrin and comparing said counts with the number of counts from the coated test apparatus prepared in Step (a).
Claim 2
Independent Claim
177 words
A method for detecting and measuring enzymes capable of digesting fibrinogen and for detecting and measuring activators and inhibitors of enzymes capable of digesting fibrinogen which comprises: a. coating at room temperature a water-insoluble polymer test apparatus with a buffered solution of a radioactive tracer labelled enzyme-free human fibrinogen to produce a moist coating; b. reacting said moist coating with a thrombin solution thereby converting the labelled fibrinogen to labelled fibrin; c. contacting said coating of radioactively labelled fibrin with a sample of the unknown enzyme or a mixture of a known enzyme capable of digesting fibrin and the unknown enzyme activator or a mixture of a known enzyme capable of digesting fibrin and the unknown enzyme inhibitor; d. incubating said sample in contact with said coating; e. washing the test apparatus with a buffered solution and recovering the solution containing the radioactively digested products released by said sample; f. counting radiation emitted by said digested and released fibrin and comparing said counts with the number of counts from the coated test apparatus prepared in Step (a).
Claim 3
Dependent Claim
12 words
The method of claim 1, wherein said unknown enzyme sample is blood.
Claim 4
Dependent Claim
14 words
The method of claim 1, wherein said unknown enzyme sample is a biological fluid.
Claim 5
Dependent Claim
18 words
The method of claim 1, wherein said unknown enzyme sample is a solution of at least one enzyme.
Claim 6
Dependent Claim
31 words
The method of claim 1, wherein said unknown enzyme sample is a known amount of an enzyme in admixture with an unknown sample containing inhibitors of activators of said known enzyme.
Claim 7
Independent Claim
163 words
A method for detecting and measuring enzymes capable of digesting fibrinogen and for detecting and measuring activators and inhibitors of enzymes capable of digesting fibrinogen which comprises: a. coating at room temperature a water-insoluble polymer test apparatus with a buffered solution of a radioactive tracer labelled enzyme-free human fibrinogen to produce a moist coating; b. contacting said moist coating of radioactively labelled fibrinogen with a sample of the unknown enzyme or a mixture of a known enzyme capable of digesting fibrinogen and the unknown enzyme activator or a mixture of a known enzyme capable of digesting fibrinogen and the unknown enzyme inhibitor; c. incubating said sample in contact with said coating; d. washing the test apparatus with a buffered solution and recovering the solution containing the radioactive digested products released by said sample; e. counting radiation emitted by said digested and released products of fibrinogen and comparing said counts with the number of counts from the coated test apparatus prepared in Step (a).
Claim 8
Independent Claim
163 words
A method for detecting and measuring enzymes capable of digesting fibrinogen and for detecting and measuring activators and inhibitors of enzymes capable of digesting fibrinogen which comprises: a. coating at room temperature a water-insoluble polymer test apparatus with a buffered solution of a radioactive tracer labelled enzyme-free human fibrinogen to produce a moist coating; b. contacting said moist coating of radioactively labelled fibrinogen with a sample of the unknown enzyme or a mixture of a known enzyme capable of digesting fibrinogen and the unknown enzyme activator or a mixture of a known enzyme capable of digesting fibrinogen and the unknown enzyme inhibitor; c. incubating said sample in contact with said coating; d. washing the test apparatus with a buffered solution and recovering the solution containing the radioactive digested products released by said sample; e. counting radiation emitted by said digested and released products of fibrinogen and comparing said counts with the number of counts from the coated test apparatus prepared in Step (a).
Claim 9
Dependent Claim
13 words
The method of claim 6, wherein the unknown enzyme sample is blood. radioactive
Claim 10
Dependent Claim
14 words
The method of claim 6, wherein the unknown enzyme sample is a biological fluid.
Claim 11
Dependent Claim
18 words
The method of claim 6, wherein the unknown enzyme sample is a solution of at least one enzyme.
Claim 12
Dependent Claim
31 words
The method of claim 6, wherein the unknown enzyme sample is a known amount of an enzyme in admixture with an unknown sample containing inhibitors or activators of said known enzyme.
References Cited (Backward Citations)
US3639558A
Cited
US3778352A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-05 | 1981-03-07 | 1981-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-05 | 1985-03-07 | 1985-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-05 | 1989-03-07 | 1989-09-06 | $7700.00 | Window Closed / Lapsed |