US4045290A
Expired / Lapsed
US
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Diagnostic method and compounds for use therewith
Assignee: Becton Dickinson and Co
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Filing: 1976-02-18
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Granted: 1977-08-30
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Expires: 1996-02-18
Abstract
Abstract An assay for phosphatase enzymes in body fluids comprising contacting a sample of such body fluid with the substrate, Thymol Blue Monophosphate, which is chromogenic at the pH of the reaction mixture and determining activity of the enzyme by measuring the rate of formation of the Thymol Blue dye.
Inventors
- 👤 George F. Bulbenko
- 👤 Joseph Eliot Woodbridge
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 06:19
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/42; C07; C07F; C07F9/00; C07F9/02; C07F9/547; C07F9/655; C07F9/65515; C07F9/65517; C12Q2334/00; C12Q2334/40
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
36 words
Compounds useful in the assay of alkaline phosphatase enzymes comprising a compound having the structure ##STR3## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2 -amino-2-methyl 1,3-propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) amminomethane.
Claim 2
Dependent Claim
36 words
Compounds useful in the assay of alkaline phosphatase enzymes comprising a compound having the structure ##STR3## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2 -amino-2-methyl 1,3-propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) amminomethane.
Claim 3
Dependent Claim
18 words
The compound of claim 1 wherein R is selected from the group consisting of sodium, potassium and ammonium.
Claim 4
Dependent Claim
65 words
A composition for the assay of alkaline phosphatase enzymes comprising a compound having the structure ##STR4## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2 -amino-2-methyl 1,3-propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase in an amount sufficient to activate said alkaline phosphatase.
Claim 5
Dependent Claim
65 words
A composition for the assay of alkaline phosphatase enzymes comprising a compound having the structure ##STR4## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2 -amino-2-methyl 1,3-propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase in an amount sufficient to activate said alkaline phosphatase.
Claim 6
Dependent Claim
16 words
The composition of claim 3 wherein R is selected from the group sodium, potassium and ammonium.
Claim 7
Dependent Claim
11 words
The composition of claim 3 wherein said phosphorylatable buffer is diethanolamine.
Claim 8
Dependent Claim
13 words
The composition of claim 3 wherein said activator is an inorganic magnesium compound.
Claim 9
Independent Claim
92 words
A method of assaying alkaline phosphatase enzymes present in a sample comprising contacting said sample with a composition which releases a dyestuff at a measurable rate, said composition comprising a compound having the structure ##STR5## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1,3-propanediol, dicylohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase enzymes in an amount sufficient to activate said enzymes and spectrophotometrically measuring the rate of formation of dye.
Claim 10
Independent Claim
92 words
A method of assaying alkaline phosphatase enzymes present in a sample comprising contacting said sample with a composition which releases a dyestuff at a measurable rate, said composition comprising a compound having the structure ##STR5## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1,3-propanediol, dicylohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase enzymes in an amount sufficient to activate said enzymes and spectrophotometrically measuring the rate of formation of dye.
Claim 11
Independent Claim
83 words
A method of assaying alkaline phosphatase enzymes present in a sample comprising contacting said sample with a composition comprising a compound having the structure ##STR6## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1-3propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase enzymes in an amount sufficient to activate said enzymes, adding alkali, and determining the absorbance of the resulting composition.
Claim 12
Independent Claim
83 words
A method of assaying alkaline phosphatase enzymes present in a sample comprising contacting said sample with a composition comprising a compound having the structure ##STR6## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1-3propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase enzymes in an amount sufficient to activate said enzymes, adding alkali, and determining the absorbance of the resulting composition.
Claim 13
Independent Claim
67 words
A method of assaying acid phosphatase enzyme present in a sample comprising contacting said sample with a composition comprising a compound having the structure ##STR7## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1,3- propanediol, dicyclohexylamine, cyclohexylamine and tris (hydroxymethyl) aminomethane, a buffer in an amount sufficient to produce an acid pH, adding alkali and determining the absorbance of the resulting composition.
Claim 14
Independent Claim
67 words
A method of assaying acid phosphatase enzyme present in a sample comprising contacting said sample with a composition comprising a compound having the structure ##STR7## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1,3- propanediol, dicyclohexylamine, cyclohexylamine and tris (hydroxymethyl) aminomethane, a buffer in an amount sufficient to produce an acid pH, adding alkali and determining the absorbance of the resulting composition.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-29 | 1981-02-28 | 1981-08-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-29 | 1985-02-28 | 1985-08-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-29 | 1989-02-28 | 1989-08-30 | $7700.00 | Window Closed / Lapsed |