US4044257A
Expired / Lapsed
US
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Retention time chromatograph employing an IR absorption spectrographic detector
Assignee: Diax Corp
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Filing: 1975-02-20
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Granted: 1977-08-23
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Expires: 1995-02-20
Abstract
Abstract Sample material of retention time peaks of a chromatograph are exposed to infrared radiation at first and second wavelengths and the absorption of the infrared energy by the sample peak is measured. The measured infrared absorption at the first and second wavelengths is correlated with the retention time of the sample peak as an identification of the sample material. In addition, overlapping retention time peaks are resolved by detecting the change in the ratio of the infrared absorption at the first and second wavelengths as a function of retention time for a given peak.
Inventors
- 👤 Lloyd B. Kreuzer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:58
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/62; G01N30/74; G01J; G01J3/00; G01J3/28; G01J3/42; G01J3/427
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
189 words
In a method of identifying the sample material of a retention peak of a retention time chromatograph, the steps of: determining the retention time of a retention peak of a sample constituent in the effluent of a retention time chromatograph; detecting the absorption of infrared energy by the sample material of the retention peak from a laser beam at first and second infrared wavelengths of the laser beam; controlling the pressure of the sample constituent material to a predetermined value in a region wherein the absorption of the infrared energy from the laser beam is detected; and correlating the retention time of the sample peak with the detected absorption of the sample peak at said first and second infrared wavelengths to identify the sample constituent of the retention peak and deriving the ratio of the infrared absorption of the sample material of said retention peak at said first and second wavelengths; and wherein the step of correlating the retention time of the sample peak with the absorption of the sample at said first and second wavelengths comprises correlating said ratio with the retention time to identify the sample constituent.
Claim 2
Dependent Claim
189 words
In a method of identifying the sample material of a retention peak of a retention time chromatograph, the steps of: determining the retention time of a retention peak of a sample constituent in the effluent of a retention time chromatograph; detecting the absorption of infrared energy by the sample material of the retention peak from a laser beam at first and second infrared wavelengths of the laser beam; controlling the pressure of the sample constituent material to a predetermined value in a region wherein the absorption of the infrared energy from the laser beam is detected; and correlating the retention time of the sample peak with the detected absorption of the sample peak at said first and second infrared wavelengths to identify the sample constituent of the retention peak and deriving the ratio of the infrared absorption of the sample material of said retention peak at said first and second wavelengths; and wherein the step of correlating the retention time of the sample peak with the absorption of the sample at said first and second wavelengths comprises correlating said ratio with the retention time to identify the sample constituent.
Claim 3
Dependent Claim
45 words
The method of claim 1 including the step of, detecting time dependent changes in the correlation of the detected absorption of the sample peak at said first and second wavelengths as a function of retention time within a given peak to resolve overlapping retention peaks.
Claim 4
Dependent Claim
29 words
The method of claim 1 including the step of detecting changes in said ratio as a function of retention time within a given peak to resolve overlapping retention peaks.
Claim 5
Independent Claim
108 words
The method for resolving constituents of a fluid sample including the steps of: deriving a pair of sample absorption signals which are a function of the absorption of infrared energy by constituents of the sample material from a laser beam of infrared radiation at first and second wavelengths λ 1 and λ 2 , respectively; controlling the pressure of the fluid sample to a predetermined value in the region where the absorption signals are derived; and deriving an output proportional to the ratio of the two sample absorption signals at λ 1 and λ 2 said output being characteristic of the constituents in the fluid sample under analysis.
Claim 6
Independent Claim
108 words
The method for resolving constituents of a fluid sample including the steps of: deriving a pair of sample absorption signals which are a function of the absorption of infrared energy by constituents of the sample material from a laser beam of infrared radiation at first and second wavelengths λ 1 and λ 2 , respectively; controlling the pressure of the fluid sample to a predetermined value in the region where the absorption signals are derived; and deriving an output proportional to the ratio of the two sample absorption signals at λ 1 and λ 2 said output being characteristic of the constituents in the fluid sample under analysis.
Claim 7
Dependent Claim
25 words
The method of claim 4 including the step of displaying at least one of the absorption signal outputs at λ 1 and λ 2 .
Claim 8
Dependent Claim
43 words
The method of claim 4 including the step of deriving the ratio of the sample absorption signals as a function of time to detect time dependent changes in said ratio, such changes being indicative of changes in constituents of the sample under analysis.
Claim 9
Dependent Claim
49 words
The method of claim 6 wherein the fluid sample to be analyzed is the effluent stream of a retention time chromatograph and including the step of, correlating the derived ratio as a function of time with retention peaks of the retention time chromatograph for resolving overlapping chromatograph signal peaks.
Claim 10
Dependent Claim
176 words
Apparatus for identifying sample constituents of a retention peak of a retention time chromatograph including: means for determining the retention time of retention peak of a sample constituent in the effluent of a retention time chromatograph; means for detecting the infrared absorption of the sample material of the retention peak at first and second infrared wavelengths of coherent radiation; means for controlling the pressure of the sample material to a predetermined value in the infrared detection region where the absorption is detected; and means for correlating the retention time of the sample peak with the detected absorption of the sample peak at said first and second infrared wavelengths to identify the sample constituent and means for deriving the ratio of the absorption of the sample material of said retention peak at said first and second wavelengths; and wherein said means for correlating the retention time of the sample peak with the absorption of the sample at said first and second wavelengths comprises means for correlating said ratio with the retention time to identify the sample constituent
Claim 11
Dependent Claim
176 words
Apparatus for identifying sample constituents of a retention peak of a retention time chromatograph including: means for determining the retention time of retention peak of a sample constituent in the effluent of a retention time chromatograph; means for detecting the infrared absorption of the sample material of the retention peak at first and second infrared wavelengths of coherent radiation; means for controlling the pressure of the sample material to a predetermined value in the infrared detection region where the absorption is detected; and means for correlating the retention time of the sample peak with the detected absorption of the sample peak at said first and second infrared wavelengths to identify the sample constituent and means for deriving the ratio of the absorption of the sample material of said retention peak at said first and second wavelengths; and wherein said means for correlating the retention time of the sample peak with the absorption of the sample at said first and second wavelengths comprises means for correlating said ratio with the retention time to identify the sample constituent
Claim 12
Dependent Claim
47 words
The apparatus of claim 8 including, means for detecting time dependent changes in the correlation of the detected absorption of the sample peak at said first and second infrared wavelengths as a function of retention time within a given retention time peak to resolve overlapping retention peaks.
Claim 13
Dependent Claim
28 words
The apparatus of claim 8 including means for detecting changes in said ratio as a function of retention time within a given peak to resolve overlappig retention peaks.
Claim 14
Dependent Claim
136 words
In an apparatus for detecting constituents of a fluid sample employing the principle of absorption of infrared energy by the constituents in the fluid sample from an incident beam of infrared radiation: means for deriving a pair of sample absorption signals which are a function of the absorption of infrared energy by constituents of the sample from an infrared laser beam of energy at first and second wavelengths λ 1 and λ 2 , respectively; means for controlling the pressure of the sample to a predetermined value in the region where the absorption of infrared energy by the sample is obtained; and means for deriving an output proportional to the ratio of the two sample absorption signals at λ 1 and λ 2 said output being characteristic of the constituents in the fluid sample under analysis.
Claim 15
Dependent Claim
136 words
In an apparatus for detecting constituents of a fluid sample employing the principle of absorption of infrared energy by the constituents in the fluid sample from an incident beam of infrared radiation: means for deriving a pair of sample absorption signals which are a function of the absorption of infrared energy by constituents of the sample from an infrared laser beam of energy at first and second wavelengths λ 1 and λ 2 , respectively; means for controlling the pressure of the sample to a predetermined value in the region where the absorption of infrared energy by the sample is obtained; and means for deriving an output proportional to the ratio of the two sample absorption signals at λ 1 and λ 2 said output being characteristic of the constituents in the fluid sample under analysis.
Claim 16
Dependent Claim
12 words
The apparatus of claim 11 including means for displaying said ratio signal.
Claim 17
Dependent Claim
46 words
The apparatus of claim 11 including means for deriving said output proportional to the ratio of the sample absorption signals as a function of time to detect time dependent changes in said ratio which are indicative of changes in the constituents of the sample under analysis.
Claim 18
Dependent Claim
54 words
The apparatus of claim 13 including retention time chromatograph means having an effluent stream to be analyzed; detector means for detecting the sample constituents as a function of retention time; and wherein said means for deriving the pair of sample absorption signals derives said signals from the effluent stream of the retention time chromatograph.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-22 | 1981-02-21 | 1981-08-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-22 | 1985-02-21 | 1985-08-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-22 | 1989-02-21 | 1989-08-23 | $7700.00 | Window Closed / Lapsed |