US4038778A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Tissue culture technique for asexual plant reproduction and a medium for use therewith
Assignee: Verizon Laboratories Inc
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Filing: 1976-09-27
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Granted: 1977-08-02
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Expires: 1996-09-27
Abstract
Abstract A tissue culture technique and a culture medium used therewith for asexually propagating a plurality of plants of the family Cruciferae are disclosed.
Inventors
- 👤 Prakash G. Kadkade
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 13:25
CPC Classifications
A; A01; A01H; A01H4/00; A01H4/005; A01H4/008
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
76 words
A method for asexually propagating plants comprising the steps of: a. excising a part of the donor plant; b. culturing the excised part on a nutrient medium containing 0.5 to 1mg/l of IAA, 0.1 to 0.5 mg/l of kinetin, and 20-40 mg/l of a salt of adenine to promote the simultaneous growth of shoots and roots and induce the differentiation of the excised part into a plurality of plantlets; and c. transplanting the plantlets into soil.
Claim 2
Independent Claim
76 words
A method for asexually propagating plants comprising the steps of: a. excising a part of the donor plant; b. culturing the excised part on a nutrient medium containing 0.5 to 1mg/l of IAA, 0.1 to 0.5 mg/l of kinetin, and 20-40 mg/l of a salt of adenine to promote the simultaneous growth of shoots and roots and induce the differentiation of the excised part into a plurality of plantlets; and c. transplanting the plantlets into soil.
Claim 3
Dependent Claim
40 words
The method of claim 1 wherein the simultaneous shoot and root formation is promoted by culturing the excised plant part on a medium containing approximately 1.0 mg/l of IAA, 0.5 mg/l of kinetin and 20 to 40 mg/l adenine sulfate.
Claim 4
Dependent Claim
19 words
The method of claim 2 wherein the donor plant is lettuce and the excised part is from the cotyledon.
Claim 5
Dependent Claim
15 words
The method of claim 2 wherein the donor plant is 3 to 5 days old.
Claim 6
Dependent Claim
15 words
The method of claim 1 wherein the culture is illuminated daily with broad spectrum light.
Claim 7
Dependent Claim
23 words
The method of claim 5 wherein the culture is subjected to a photoperiod of 16 hours of illumination and eight hours of darkness.
Claim 8
Independent Claim
88 words
A method for asexually propagating plants comprising the steps of: a. excising a portion of a donor plant; b. culturing the excised portion in a first nutrient medium to promote callus formation; c. culturing the callus onto a second nutrient medium containing -0.5 to 1 mg/l of IAA, 0.1 to 0.5 mg/l of kinetin, and 20 to 40 mg/l of a salt of adenine to promote the differentiation of the callus into shoots and roots which form a plurality of plantlets; and d. transplanting the plantlets into soil.
Claim 9
Independent Claim
88 words
A method for asexually propagating plants comprising the steps of: a. excising a portion of a donor plant; b. culturing the excised portion in a first nutrient medium to promote callus formation; c. culturing the callus onto a second nutrient medium containing -0.5 to 1 mg/l of IAA, 0.1 to 0.5 mg/l of kinetin, and 20 to 40 mg/l of a salt of adenine to promote the differentiation of the callus into shoots and roots which form a plurality of plantlets; and d. transplanting the plantlets into soil.
Claim 10
Dependent Claim
16 words
The method of claim 7 wherein the first medium contains naphthaleneacetic acid and N 6 -Benzyladenine.
Claim 11
Dependent Claim
24 words
The method of claim 8 wherein the second medium contains -1.0 mg/l of IAA, 0.5 mg/l of kinetin, and 40 mg/l of adenine sulfate.
Claim 12
Dependent Claim
25 words
The method of claim 7 including the intermediate step of storing the formed callus to timely initiate the formation of plantlets as the need arises.
Claim 13
Dependent Claim
42 words
A tissue culture medium for use in the simultaneous formation of shoot and root by an excised plant part comprising suitable nutrients 0.5 to 1.0 mg/l of IAA, 0.1 to 0.5 mg/l of kinetin and 20 to 40 mg/l of adenine sulfate.
Claim 14
Dependent Claim
42 words
A tissue culture medium for use in the simultaneous formation of shoot and root by an excised plant part comprising suitable nutrients 0.5 to 1.0 mg/l of IAA, 0.1 to 0.5 mg/l of kinetin and 20 to 40 mg/l of adenine sulfate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-01 | 1981-01-31 | 1981-08-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-01 | 1985-01-31 | 1985-08-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-01 | 1989-01-31 | 1989-08-02 | $7700.00 | Window Closed / Lapsed |