US4030978A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel assembly, compositions and methods
Assignee: Becton Dickinson and Co
•
Filing: 1976-07-29
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Granted: 1977-06-21
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Expires: 1996-07-29
Abstract
Abstract The disclosure is of a novel assembly and method for transporting aerobic, anaerobic and facultative microorganisms from clinical patient to laboratory. The disclosure is also of a novel protective medium for maintaining viability of microorganisms during transportation.
Inventors
- 👤 Irwin J. Abramson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 13:14
CPC Classifications
C; C12; C12M; C12M33/00; C12M33/02; C12M45/00; C12M45/22; C12N; C12N1/00; C12N1/04
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
126 words
An assembly for maintaining anaerobic, aerobic or facultative microorganisms, which comprises; a tubular container having a first, hermetically sealed end and a second end hermetically sealed with a removable closure, said container with said closure defining a collection chamber; a composition partially filling said collection chamber, said composition comprising, in parts by weight, 1,000 parts ion free water 0.01-0.5 parts oxygen indicator 1.8-2.2 parts sodium phosphate, tribasic 0.9-1.1 parts potassium phosphate, dibasic 0.09-0.11 parts calcium chloride 0.09-0.11 parts magnesium chloride hexahydrate 7.5-8.5 parts sodium chloride 0.5-1.5 parts sodium thioglycolate 0.5-1.5 parts cysteine hydrochloride 5-20 parts agar; said composition having a consistency of from about 170-350 grams/cm 2 and having a pH of 6.9-7.8; the remaining portion of said chamber being filled with an oxygen free gas.
Claim 2
Dependent Claim
126 words
An assembly for maintaining anaerobic, aerobic or facultative microorganisms, which comprises; a tubular container having a first, hermetically sealed end and a second end hermetically sealed with a removable closure, said container with said closure defining a collection chamber; a composition partially filling said collection chamber, said composition comprising, in parts by weight, 1,000 parts ion free water 0.01-0.5 parts oxygen indicator 1.8-2.2 parts sodium phosphate, tribasic 0.9-1.1 parts potassium phosphate, dibasic 0.09-0.11 parts calcium chloride 0.09-0.11 parts magnesium chloride hexahydrate 7.5-8.5 parts sodium chloride 0.5-1.5 parts sodium thioglycolate 0.5-1.5 parts cysteine hydrochloride 5-20 parts agar; said composition having a consistency of from about 170-350 grams/cm 2 and having a pH of 6.9-7.8; the remaining portion of said chamber being filled with an oxygen free gas.
Claim 3
Dependent Claim
19 words
The assembly of claim 1 wherein said consistency (gel strength) is from about 180 to 190 gms/cm 2 .
Claim 4
Dependent Claim
15 words
The assembly of claim 1 wherein said gas consists of 95% nitrogen and 5% hydrogen.
Claim 5
Dependent Claim
11 words
The assembly of claim 1 wherein said oxygen indicator is resazurin.
Claim 6
Dependent Claim
53 words
The assembly of claim 1 wherein said composition consists essentially of 1,000 parts ion free water 0.002 parts resazurin 2.0 parts sodium phosphate tribasic 1.0 parts potassium phosphate dibasic 0.1 parts calcium chloride 0.1 parts magnesium chloride hexahydrate 8.0 parts sodium chloride 1.0 parts sodium thioglycolate 1.0 parts cysteine hydrochloride 10.0 parts agar.
Claim 7
Dependent Claim
8 words
A composition, which comprises; in parts by weight,
Claim 8
Dependent Claim
8 words
A composition, which comprises; in parts by weight,
Claim 9
Dependent Claim
43 words
000 parts ion free water 0.01-0.5 parts oxygen indicator 1.8-2.2 parts sodium phosphate, tribasic 0.9-1.1 parts potassium phosphate, dibasic 0.09-0.11 parts calcium chloride 0.09-0.11 parts magnesium chloride hexahydrate 7.5-8.5 parts sodium chloride 0.5-1.5 parts sodium thioglycolate 0.5-1.5 parts cysteine hydrochloride 5-20 parts agar.
Claim 10
Dependent Claim
43 words
000 parts ion free water 0.01-0.5 parts oxygen indicator 1.8-2.2 parts sodium phosphate, tribasic 0.9-1.1 parts potassium phosphate, dibasic 0.09-0.11 parts calcium chloride 0.09-0.11 parts magnesium chloride hexahydrate 7.5-8.5 parts sodium chloride 0.5-1.5 parts sodium thioglycolate 0.5-1.5 parts cysteine hydrochloride 5-20 parts agar.
Claim 11
Dependent Claim
12 words
A composition according to claim 6 wherein said oxygen indicator is resazurin.
Claim 12
Dependent Claim
54 words
A composition according to claim 6, which comprises; in parts by weight, 1,000 parts ion free water 0.002 parts resazurin 2.0 parts sodium phosphate tribasic 1.0 parts potassium phosphate dibasic 0.1 parts calcium chloride 0.1 parts magnesium chloride hexahydrate 8.0 parts sodium chloride 1.0 parts sodium thioglycolate 1.0 parts cysteine hydrochloride 10.0 parts agar.
Claim 13
Independent Claim
157 words
A method of maintaining viability of collected anaerobic, aerobic or facultative microorganisms, which comprises; providing an assembly, which comprises; a tubular container having a first, hermetically sealed end and a second end hermetically sealed with a removable closure, said container with said closure defining a collection chamber; a composition partially filling said collection chamber, said composition comprising, in parts by weight, 1,000 parts ion free water 0.01-0.5 parts oxygen indicator 1.8-2.2 parts sodium phosphate, tribasic 0.9-1.1 parts potassium phosphate, dibasic 0.09-0.11 parts calcium chloride 0.09-0.11 parts magnesium chloride hexahydrate. 7.5-8.5 parts sodium chloride 0.5-1.5 parts sodium thioglycolate 0.5-1.5 parts cysteine hydrochloride 5-20 parts agar; said composition having a consistency (gel strength) of from about 170-350 gms/cm 2 and having a pH of 6.9-7.8; the unfilled portion of said chamber being filled with an oxygen free gas; depositing a clinical specimen suspected of harboring pathogenic organisms into said chamber; and hermetically sealing the open end of said container.
Claim 14
Independent Claim
157 words
A method of maintaining viability of collected anaerobic, aerobic or facultative microorganisms, which comprises; providing an assembly, which comprises; a tubular container having a first, hermetically sealed end and a second end hermetically sealed with a removable closure, said container with said closure defining a collection chamber; a composition partially filling said collection chamber, said composition comprising, in parts by weight, 1,000 parts ion free water 0.01-0.5 parts oxygen indicator 1.8-2.2 parts sodium phosphate, tribasic 0.9-1.1 parts potassium phosphate, dibasic 0.09-0.11 parts calcium chloride 0.09-0.11 parts magnesium chloride hexahydrate. 7.5-8.5 parts sodium chloride 0.5-1.5 parts sodium thioglycolate 0.5-1.5 parts cysteine hydrochloride 5-20 parts agar; said composition having a consistency (gel strength) of from about 170-350 gms/cm 2 and having a pH of 6.9-7.8; the unfilled portion of said chamber being filled with an oxygen free gas; depositing a clinical specimen suspected of harboring pathogenic organisms into said chamber; and hermetically sealing the open end of said container.
Claim 15
Dependent Claim
52 words
A method according to claim 9 wherein said composition comprises; 1,000 parts ion free water 0.002 parts resazurin 2.0 parts sodium phosphate tribasic 1.0 parts potassium phosphate dibasic 0.1 parts calcium chloride 0.1 parts magnesium chloride hexahydrate 8.0 parts sodium chloride 1.0 parts sodium thioglycolate 1.0 parts cysteine hydrochloride 10.0 parts agar.
Claim 16
Dependent Claim
21 words
A method according to claim 9 wherein said depositing of the clinical specimen is underneath the upper surface of said composition.
Claim 17
Dependent Claim
16 words
A method according to claim 9 wherein said depositing is under the surface of said composition.
References Cited (Backward Citations)
US3715281A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-06-20 | 1980-12-20 | 1981-06-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-06-20 | 1984-12-20 | 1985-06-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-06-20 | 1988-12-20 | 1989-06-21 | $7700.00 | Window Closed / Lapsed |