US4026765A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production and germination of entomophthora resting spores
Assignee: United States Department of Agriculture
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Filing: 1976-04-30
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Granted: 1977-05-31
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Expires: 1996-04-30
Abstract
Abstract Product of Entomophthora resting spores was increased by 50 to 70% by modifying an egg yolk media with a particular maltose agar. Germination of the resisting spores at levels up to 100% was obtained by preconditioning harvested spores by treatment with 95% ethanol, high speed blending, sonication, or a combination of ethanol treatment and high speed blending. Germination of spores which had been dried and stored but not pretreated as above was greatly increased by exposure to an atmosphere of 95% ethanol. In addition, treatment by the processes of this invention resulted in the production of a spore stage not previously observed or reported. Spores thus produced have been termed germ conidia.
Inventors
- 👤 Richard S. Soper, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 02:09
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/14; C12N3/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
44 words
In a process for producing Entomophthora resting spores wherein a standard egg yolk media is inoculated with a culture of the Entomophthora spore, the improvement comprising adding to and mixing with the egg yolks during preparation of the media, a molten Sabourad maltose agar.
Claim 2
Dependent Claim
44 words
In a process for producing Entomophthora resting spores wherein a standard egg yolk media is inoculated with a culture of the Entomophthora spore, the improvement comprising adding to and mixing with the egg yolks during preparation of the media, a molten Sabourad maltose agar.
Claim 3
Dependent Claim
26 words
The improvement of claim 1 wherein the media is inoculated with Entomophthora spores and the number of resting spores produced is increased by 50 to 70%.
Claim 4
Dependent Claim
14 words
The improvement of claim 2 wherein the media is inoculated with E. thaxteriana spores.
Claim 5
Dependent Claim
14 words
The improvement of claim 2 wherein the media is inoculated with E. aphidis spores.
Claim 6
Dependent Claim
14 words
The improvement of claim 2 wherein the media is inoculated with E. blullata spores.
Claim 7
Dependent Claim
14 words
The improvement of claim 2 wherein the media is inoculated with E. exitialis spores.
Claim 8
Dependent Claim
14 words
The improvement of claim 2 wherein the media is inoculated with E. grylli spores.
Claim 9
Dependent Claim
14 words
The improvement of claim 2 wherein the media is inoculated with E. virulenta spores.
Claim 10
Dependent Claim
20 words
The improvement of claim 2 wherein the media is inoculated with an Entomophthora sp. from spruce budworm Choristoneura fumiferana (Clemens).
References Cited (Backward Citations)
US3000791A
Cited
US3294647A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-30 | 1980-11-29 | 1981-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-30 | 1984-11-29 | 1985-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-30 | 1988-11-29 | 1989-05-31 | $7700.00 | Window Closed / Lapsed |