US4024242A
Expired / Lapsed
US
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Substance having immunological activity and process for its manufacture
Assignee: Hoechst AG
•
Filing: 1975-06-23
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Granted: 1977-05-17
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Expires: 1995-06-23
Abstract
Abstract Method of chemically attenuating trypanosomes with a phenanthridine compound, such as 3,8-diamino-5-ethyl-6-phenyl phenanthridine, to obtain non-pathogenic organisms with immunological activity; attenuated organisms produced in this way; vaccines containing such attenuated organisms.
Inventors
- 👤 Klaus-Dieter Hungerer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 02:24
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/002; A61K39/005; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
42 words
A process for the manufacture of a substance having immunological activity, which comprises incubating trypanosomes that have been suspended in a mono-phase, aqueous, liquid culture medium with a phenanthridine derivative until they lose their pathogenicity, and then collecting the trypanosomes thus attenuated.
Claim 2
Dependent Claim
42 words
A process for the manufacture of a substance having immunological activity, which comprises incubating trypanosomes that have been suspended in a mono-phase, aqueous, liquid culture medium with a phenanthridine derivative until they lose their pathogenicity, and then collecting the trypanosomes thus attenuated.
Claim 3
Dependent Claim
15 words
A process as claimed in claim 1, wherein the phenanthridine derivative used is 3,8-diamino-5-ethyl-6-phenyl-phenanthridinium bromide.
Claim 4
Dependent Claim
15 words
A process as claimed in claim 1, wherein the culture medium is free from proteins.
Claim 5
Dependent Claim
23 words
A process as claimed in claim 1, wherein the composition of trypanosomes in the suspension ranges from 10 3 to 10 8 /ml.
Claim 6
Dependent Claim
20 words
A process as claimed in claim 1, wherein the phenanthridine derivative is used in a concentration of 0.5 to 1000γ/ml.
Claim 7
Dependent Claim
22 words
A process as claimed in claim 1, wherein incubation is carried out for 1 to 120 hours at 18° - 37° C.
Claim 8
Dependent Claim
27 words
A process as claimed in claim 1, wherein the concentration of trypanosomes in the suspension ranges from 1 × 10 7 to 5 × 10 7 /ml.
Claim 9
Dependent Claim
20 words
A process as claimed in claim 1, wherein the phenanthridine derivatives is used in a concentration of 5 to 100γ/ml.
Claim 10
Dependent Claim
21 words
A process as claimed in claim 1, wherein the incubation is carried out for 20 to 48 hours at 25°-33° C.
Claim 11
Dependent Claim
9 words
Chemically attenuated trypanosomes as obtained according to claim 1.
Claim 12
Dependent Claim
19 words
A vaccine effective against the Chagas disease, containing attenuated trypanosomes as claimed in claim 10 as the active ingredient.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-16 | 1980-11-15 | 1981-05-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-16 | 1984-11-15 | 1985-05-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-16 | 1988-11-15 | 1989-05-17 | $7700.00 | Window Closed / Lapsed |