US4021302A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell cultures
Assignee: SmithKline Beecham Corp
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Filing: 1975-08-20
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Granted: 1977-05-03
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Expires: 1995-08-20
Abstract
Abstract Cell culture comprising a novel cell strain in suitable nutrient medium, wherein the novel cell strain is produced by disaggregated feline embryo tissue cell and serially passaging the same from about the 20th to about the 48th passage level, so as to be cultured and subcultured with the resultant cell strains remaining substantially diploid, typically fibroblastic and substantially free from morphological transformations. Subculturing is facilitated by treatment of a confluent sheet of the disaggregated cells chelating agent before each transfer into a new batch of nutrient medium.
Inventors
- 👤 Sidney Edwin Smith
- 👤 Kevin Joseph O'Reilly
- 👤 John Prydie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 12:22
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61K; A61K39/00; A61K39/12; A61K39/23; A61K39/245; A61K39/265; A61K2039/51; A61K2039/525; A61K2039/5254; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16711; C12N2710/16734; C12N2750/00; C12N2750/00011; C12N2750/14011; C12N2750/14034; C12N2750/14061; C12N2750/14064; C12N2750/14311; C12N2750/14334; C12N2750/14361; C12N2750/14364
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
136 words
A cell culture comprising a cell strain and a suitable nutrient medium therefor, wherein said cell strain is a feline embryo cell strain produced by disaggregating by chemical or mechanical means feline embryo tissue cells susceptible to feline infectious enteritis virus and serially passaging said cells up to at least the 20th passage level and at most the 48th passage level in a suitable nutrient media, said nutrient medium having a pH between 6.8-7.8 and a temperature in the range of 32° C. to 39° C., so as to be cultured and sub-cultured, wherein said sub-culturing is facilitated by treating the confluent sheet of said cells with a chelating agent before each transfer into a new batch of suitable nutrient medium, said resultant cell strains remaining substantially diploid, typically fibroblastic, and substantially free from morphological transformations.
Claim 2
Dependent Claim
136 words
A cell culture comprising a cell strain and a suitable nutrient medium therefor, wherein said cell strain is a feline embryo cell strain produced by disaggregating by chemical or mechanical means feline embryo tissue cells susceptible to feline infectious enteritis virus and serially passaging said cells up to at least the 20th passage level and at most the 48th passage level in a suitable nutrient media, said nutrient medium having a pH between 6.8-7.8 and a temperature in the range of 32° C. to 39° C., so as to be cultured and sub-cultured, wherein said sub-culturing is facilitated by treating the confluent sheet of said cells with a chelating agent before each transfer into a new batch of suitable nutrient medium, said resultant cell strains remaining substantially diploid, typically fibroblastic, and substantially free from morphological transformations.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-02 | 1980-11-01 | 1981-05-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-02 | 1984-11-01 | 1985-05-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-02 | 1988-11-01 | 1989-05-03 | $7700.00 | Window Closed / Lapsed |