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US4014612A Expired / Lapsed US ⏱️ 0.0 years remaining

Photometric measuring apparatus

Assignee: Applied Biosystems Inc
•
Filing: 1974-08-22
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Granted: 1977-03-29
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Expires: 1994-08-22
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Abstract

Abstract A highly stable and sensitive photometric apparatus is obtained by combining a sensitive, stable, unchopped, dual-beam optical photometer with an accurate electronic sensing circuit. The circuit includes PIN silicon diode detectors on which the two beams of the photometer are imaged. One beam is a reference to detect light source variations and the other a signal beam passing through the sample cell of the photometer. The PIN diode outputs are inputs to parametric amplifiers. The amplifier outputs are then provided to log taking means which take the logarithms of the two signals and then take the difference of the two logarithms to provide a final output proportional to absorbtion in the cell.

Inventors

  • 👤 John G. Atwood
  • 👤 Hamilton W. Marshall, Jr.
  • 👤 II Charles F. DEMey
  • 👤 Wilson P. Ralston

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-28 15:52
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/251; G01J; G01J1/00; G01J1/10; G01J1/16; G01J1/1626

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 244 words
In photometric apparatus for the type wherein absorbance through a sample cell in one of two radiation paths is determined by directing light from a source of predetermined wavelength contemporaneously along both paths, the improvement comprising: a first PIN diode disposed at the end of the path with the sample cell to detect intensity of radiation thereat; a second PIN diode disposed at the end of the oher path to detect intensity of radiation thereat; a field stop, a lens, and an aperture stop sequentially aligned along an axis to receive light from the source and to pass a beam of uniform intensity over the cross-sectional area thereof; means for proportionally distributing said beam to the paths; means for focusing light from said beam distributing means to symmetrically image said field and aperture stops substantially at the entrance and exit respectively of the sample cell; means for focusing light that passes from the sample cell to symmetrically image said aperture stop wholly on the sensitive area of said first PIN diode; means for focusing light from said beam distributing means to symmetrically image one of said field and aperture stops wholly on the sensitive area of said second PIN diode; first and second parametric amplifiers separately disposed to receive the outputs from said first and second PIN diodes respectively; and circuit means for deriving signals logarithmically proportional to the output from each said parametric amplifier and for determining the difference between said logarithmic signals.
Claim 2
Dependent Claim 244 words
In photometric apparatus for the type wherein absorbance through a sample cell in one of two radiation paths is determined by directing light from a source of predetermined wavelength contemporaneously along both paths, the improvement comprising: a first PIN diode disposed at the end of the path with the sample cell to detect intensity of radiation thereat; a second PIN diode disposed at the end of the oher path to detect intensity of radiation thereat; a field stop, a lens, and an aperture stop sequentially aligned along an axis to receive light from the source and to pass a beam of uniform intensity over the cross-sectional area thereof; means for proportionally distributing said beam to the paths; means for focusing light from said beam distributing means to symmetrically image said field and aperture stops substantially at the entrance and exit respectively of the sample cell; means for focusing light that passes from the sample cell to symmetrically image said aperture stop wholly on the sensitive area of said first PIN diode; means for focusing light from said beam distributing means to symmetrically image one of said field and aperture stops wholly on the sensitive area of said second PIN diode; first and second parametric amplifiers separately disposed to receive the outputs from said first and second PIN diodes respectively; and circuit means for deriving signals logarithmically proportional to the output from each said parametric amplifier and for determining the difference between said logarithmic signals.
Claim 3
Dependent Claim 19 words
The combination of claim 1 further including means for maintaining said PIN diodes at substantially equal and constant temperatures.
Claim 4
Dependent Claim 33 words
The combination of claim 1 wherein said circuit means includes first and second logarithmic amplifiers and a differential amplifier having the inputs thereof separately connected to the respective outputs from said logarithmic amplifiers.
Claim 5
Dependent Claim 18 words
The combination of claim 3 wherein said logarithmic amplifiers and said differential amplifier each include an operational amplifier
Claim 6
Dependent Claim 19 words
The combination of claim 1 further including shutter means for simultaneously occluding light from the source along both paths.
Claim 7
Dependent Claim 74 words
The combination of claim 1 further including means for maintaining said PIN diodes at substantially equal and constant temperatures, along with shutter means for simultaneously occluding light from the source along both paths; and wherein said circuit means includes first and second logarithmic amplifiers and a differential amplifier having the inputs thereof separately connected to the respective outputs from said logarithmic amplifiers, said logarithmic amplifiers and said differential amplifier each including an operational amplifier.

References Cited (Backward Citations)

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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1980-03-28 1980-09-27 1981-03-29 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1984-03-28 1984-09-27 1985-03-29 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1988-03-28 1988-09-27 1989-03-29 $7700.00 Window Closed / Lapsed

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