US4013788A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Watersoluble extracts of corynebacteria, process for obtaining them and their use
Assignee: Bpifrance Financement SA
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Filing: 1975-04-30
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Granted: 1977-03-22
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Expires: 1995-04-30
Abstract
Abstract Watersoluble extracts of corynebacteria cells are obtained by a process which comprises the steps of subjecting bacterial residues of corynebacteria containing diaminopimelic acid, e.g. Corynebacterium parvum cells, to a mild extraction and of isolating the watersoluble extracts so obtained by physico-chemical methods. Said extracts are useful as immunological adjuvants.
Inventors
- 👤 Pierre Jolles
- 👤 Daniele Migliore-Samour
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 11:45
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/39; A61K35/00; A61K35/66; A61K35/74; C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12P; C12P1/00; C12P1/04; A61K2039/555; A61K2039/55511; A61K2039/55516; A61K2039/55588; A61K2039/55594; C12R; C12R2001/00; C12R2001/01; C12R2001/15; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/27
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
95 words
An immunological adjuvant composition comprising low molecular weight hydrosoluble fragments consisting essentially of a disaccharide-tetrapeptide, a tetrasaccharide-heptapeptide and the dimer, trimer and tetramer of the latter, said adjuvant being produced by the steps of subjecting delipidated bacterial residue of Corynebacterium Parvum containing diaminopimelic acid to grinding and homogenization in aqueous medium, centrifuging to remove undissolved solids from the aqueous medium, salting out of active material from the aqueous medium, and dialyzing said fragments containing diaminopimelic acid, having a molecular weight between 1,000 ± 200 and 8,000 ± 200 and being associated with non-aminated reducing sugars.
Claim 2
Dependent Claim
95 words
An immunological adjuvant composition comprising low molecular weight hydrosoluble fragments consisting essentially of a disaccharide-tetrapeptide, a tetrasaccharide-heptapeptide and the dimer, trimer and tetramer of the latter, said adjuvant being produced by the steps of subjecting delipidated bacterial residue of Corynebacterium Parvum containing diaminopimelic acid to grinding and homogenization in aqueous medium, centrifuging to remove undissolved solids from the aqueous medium, salting out of active material from the aqueous medium, and dialyzing said fragments containing diaminopimelic acid, having a molecular weight between 1,000 ± 200 and 8,000 ± 200 and being associated with non-aminated reducing sugars.
Claim 3
Dependent Claim
18 words
A composition according to claim 1, wherein the homogenization is effected in the presence of a non-ionic detergent.
Claim 4
Dependent Claim
14 words
A composition according to claim 1, wherein salting out is effected with ammonium sulfate.
Claim 5
Dependent Claim
16 words
A composition according to claim 1, wherein the process of production includes a purification by chromatography.
Claim 6
Dependent Claim
12 words
A composition according to claim 4, wherein chromatography is effected on cellulose.
Claim 7
Dependent Claim
18 words
A composition according to claim 1, wherein the process of production includes a filtration on a polyacrylamide gel.
Claim 8
Dependent Claim
13 words
A composition according to claim 1, wherein the process of production includes lyophilization.
References Cited (Backward Citations)
FR2189021B1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-21 | 1980-09-20 | 1981-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-21 | 1984-09-20 | 1985-03-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-21 | 1988-09-20 | 1989-03-22 | $7700.00 | Window Closed / Lapsed |